Erythrocyte-camouflaged biosensor for α-hemolysin detection.
Biosensors & bioelectronics, 2021 Without appropriate treatment, Staphylococcus aureus (S. aureus) infection can cause life-threatening diseases (e.g., meningitis, pneumonia, bacteremia, and sepsis).
Insu Kim +9 more
semanticscholar +1 more source
RNase A does not translocate the alpha-hemolysin pore. [PDF]
PLoS ONE, 2014 The application of nanopore sensing utilizing the α-hemolysin pore to probe proteins at single-molecule resolution has expanded rapidly. In some studies protein translocation through the α-hemolysin has been reported. However, there is no direct evidence,
Besnik Krasniqi, Jeremy S Lee
doaj +1 more source
Redirecting Pore Assembly of Staphylococcal α-Hemolysin by Protein Engineering [PDF]
ACS Central Science, 2019 α-Hemolysin (αHL), a β-barrel pore-forming toxin (βPFT), is secreted as a water-soluble monomer by Staphylococcus aureus. Upon binding to receptors on target cell membranes, αHL assembles to form heptameric membrane-spanning pores. We have previously engineered αHL to create a protease-activatable toxin that is activated by site-specific proteolysis ...
Sunwoo Koo, Stephen Cheley, Hagan Bayley
openaire +3 more sources
Monitoring of an ATP‐Binding Aptamer and its Conformational Changes Using an α‐Hemolysin Nanopore
Small, 2011 Yi-Lun Ying, Hai-Yan Wang, Yi-Tao Long
exaly +2 more sources
Animals, 2021 A total of 318 nasal and rectal swabs were collected from 159 apparently healthy camels (Camelus dromedarius) randomly selected from five regions in southern and central Tunisia and screened for Staphylococcus aureus carriage. Staphylococcus spp.
Faten Ben Chehida +7 more
doaj +1 more source
Electrostatic Control of Polymer Translocation Speed through α-Hemolysin Protein Pore [PDF]
Macromolecules, 2016 The electrostatic origins behind the speed of translocation of a uniformly charged flexible macromolecule through α-hemolysin (αHL) protein pores under a voltage are investigated using variations in pH and electrolyte concentration. We have measured durations of successful threading of poly(styrenesulfonate) through αHL at two different pH conditions ...
Byoung-Jin, Jeon, Murugappan, Muthukumar
openaire +2 more sources
Antibiotics shaping bacterial genome: deletion of an IS91 flanked virulence determinant upon exposure to subinhibitory antibiotic concentrations. [PDF]
PLoS ONE, 2011 The nucleoid-associated proteins Hha and YdgT repress the expression of the toxin α-hemolysin. An Escherichia coli mutant lacking these proteins overexpresses the toxin α-hemolysin encoded in the multicopy recombinant plasmid pANN202-312R.
Laura Pedró +5 more
doaj +1 more source
Synergism among oxidants, proteinases, phospholipases, microbial hemolysins, cationic proteins, and cytokines [PDF]
Inflammation, 1992 A striking similarity exists between the pathogenetic properties of group A streptococci and those of activated mammalian professional phagocytes (neutrophils, macrophages). Both types of cells are endowed by the ability to adhere to target cells; to elaborate oxidants, hydrolases, and membrane-active agents (hemolysins, phospholipases); and to freely ...
Varani, James +5 more
openaire +3 more sources
Characteristics of the Protein Complexes and Pores Formed by Bacillus cereus Hemolysin BL [PDF]
Toxins, 2020 Bacillus cereus Hemolysin BL is a tripartite toxin responsible for a diarrheal type of food poisoning. Open questions remain regarding its mode of action, including the extent to which complex formation prior to cell binding contributes to pore-forming activity, how these complexes are composed, and the properties of the pores formed in the target cell
Nadja Jessberger +5 more
openaire +3 more sources
A portable lipid bilayer system for environmental sensing with a transmembrane protein. [PDF]
PLoS ONE, 2014 This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a ...
Ryuji Kawano +6 more
doaj +1 more source