Results 21 to 30 of about 6,571,241 (229)
Insights into protein sequencing with an α-Hemolysin nanopore by atomistic simulations [PDF]
Scientific Reports, 2019 AbstractSingle molecule protein sequencing would represent a disruptive burst in proteomic research with important biomedical impacts. Due to their success in DNA sequencing, nanopore based devices have been recently proposed as possible tools for the sequencing of peptide chains.
Di Muccio, Giovanni +4 more
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Characteristics of the Protein Complexes and Pores Formed by Bacillus cereus Hemolysin BL [PDF]
Toxins, 2020 Bacillus cereus Hemolysin BL is a tripartite toxin responsible for a diarrheal type of food poisoning. Open questions remain regarding its mode of action, including the extent to which complex formation prior to cell binding contributes to pore-forming activity, how these complexes are composed, and the properties of the pores formed in the target cell
Nadja Jessberger +5 more
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A portable lipid bilayer system for environmental sensing with a transmembrane protein. [PDF]
PLoS ONE, 2014 This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a ...
Ryuji Kawano +6 more
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Microorganisms, 2022 We analyzed the proteomic response of the Gram-negative fish pathogen A. salmonicida to iron limitation, an elevated incubation temperature, and the antibiotic florfenicol.
Tobias Kroniger +5 more
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Conversion of Bacterial Gene Products to Secretion-Competent Fusion Proteins
BioTechniques, 1996 We describe an efficient and easy procedure that allows the generation, detection and secretion of foreign proteins by the secretion apparatus of E. coli hemolysin.
H.-J. Mollenkopf +2 more
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Elizabethkingia anophelis: Physiologic and Transcriptomic Responses to Iron Stress
Frontiers in Microbiology, 2020 In this study, we investigated the global gene expression responses of Elizabethkingia anophelis to iron fluxes in the midgut of female Anopheles stephensi mosquitoes fed sucrose or blood, and in iron-poor or iron-rich culture conditions.
Shicheng Chen +5 more
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Subunit Dimers of α-Hemolysin Expand the Engineering Toolbox for Protein Nanopores [PDF]
Journal of Biological Chemistry, 2011 Staphylococcal α-hemolysin (αHL) forms a heptameric pore that features a 14-stranded transmembrane β-barrel. We attempted to force the αHL pore to adopt novel stoichiometries by oligomerizing subunit dimers generated by in vitro transcription and translation of a tandem gene.
Hammerstein, A, Jayasinghe, L, Bayley, H
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Pathogens, 2021 Linezolid is currently used to treat infections caused by multidrug-resistant Gram-positive cocci. Both linezolid-resistant S. aureus (LRSA) and coagulase-negative staphylococci (CoNS) strains have been collected worldwide.
Michał Michalik +5 more
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Functions of a hemolysin-like protein in the cyanobacterium Synechocystis sp. PCC 6803 [PDF]
Archives of Microbiology, 2011 A glucose-tolerant strain of the cyanobacterium Synechocystis sp. PCC 6803, generally referred to as wild type, produces a hemolysin-like protein (HLP) located on the cell surface. To analyze the function of HLP, we constructed a mutant in which the hlp gene was disrupted.
Tetsushi, Sakiyama +3 more
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A simple method to make, trap and deform a vesicle in a gel
Scientific Reports, 2023 We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double ...
Pierre Tapie +4 more
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