Thermodynamics of a Protein Acylation: Activation of Escherichia coli Hemolysin Toxin
Biochemistry, 2004 HlyC, hemolysin-activating lysine-acyltransferase, catalyses the acylation (from acyl-acyl carrier protein [ACP]) of Escherichia coli prohemolysin (proHlyA) on the epsilon-amino groups of specific lysine residues, 564 and 690 of the 1024 amino acid primary structure, to form hemolysin (HlyA).
Worsham, Lesa M.S. +2 more
openaire +4 more sources
Single-molecule chemistry studied using the protein pore -α-hemolysin
, 2012 Single-molecule detection has provided insights into how molecules behave. Without the averaging effect of ensemble measurements, the stochastic behaviour of single molecules can be observed and intermediate steps in multistep transformations can be clearly detected. The single-molecule reactants range from small molecules (e.g. propene) to proteins of
Choi, Lai-Sheung +2 more
core +5 more sources
The anti-biofilm compound 4-ethoxybenzoic acid inhibits Staphylococcus aureus virulence factor production via a putative 4EB-binding pocket in key virulence-associated proteins [PDF]
Frontiers in MicrobiologyThere is a need for dual action anti-virulence and anti-biofilm agents that target the opportunistic pathogen Staphylococcus aureus. Previous research determined that 0.8 mg/mL 4-ethoxybenzoic acid (4EB) reduced S.
Caroline C. Taylor +10 more
doaj +2 more sources
Identification of two neutralizing human single-chain variable fragment antibodies targeting Staphylococcus aureus alpha-hemolysin [PDF]
Iranian Journal of Basic Medical Sciences, 2022 Objective(s): The inability of the host immune system to defeat Staphylococcus aureus is due to various secreted virulent factors such as leukocidins, superantigens, and hemolysins, which interrupt the function of immune components.
Somayeh Piri-Gavgani +5 more
doaj +1 more source
Use of black soldier fly (Hermetia illucens) prepupae amino acids as anti Aeromonas hydrophila enterotoxin in vivo [PDF]
Iraqi Journal of Veterinary Sciences, 2023 Aeromonas hydrophila is an opportunistic freshwater. These bacteria cause gastroenteritis and septicemia in animals and humans. Hemolysin and aerolysin, are important in the pathogenesis of A. hydrophila.
Dahliatul Qosimah +5 more
doaj +1 more source
IL-21 Enhances the Immune Protection Induced by the Vibrio vulnificus Hemolysin A Protein [PDF]
Inflammation, 2021 Abstract We previously reported that the Vibrio vulnificus hemolysin A (VvhA) protein elicited good immune protection and could effectively control V. vulnificus infection in mice. However, its molecular mechanism remains unknown. In this study, we found that IL-21 enhances immune protection by inducing a Tfh-cell and germinal center (GC) B ...
Ke-Na Sun +5 more
openaire +3 more sources
Redirecting Pore Assembly of Staphylococcal α-Hemolysin by Protein Engineering [PDF]
ACS Central Science, 2019 α-Hemolysin (αHL), a β-barrel pore-forming toxin (βPFT), is secreted as a water-soluble monomer by Staphylococcus aureus. Upon binding to receptors on target cell membranes, αHL assembles to form heptameric membrane-spanning pores. We have previously engineered αHL to create a protease-activatable toxin that is activated by site-specific proteolysis ...
Sunwoo Koo, Stephen Cheley, Hagan Bayley
openaire +3 more sources
The fusion protein of peste des petits ruminants virus is a hemolysin [PDF]
Archives of Virology, 1999 The fusion glycoprotein (F protein) of paramyxoviruses plays a vital role in virus-induced cytopathology. To explore the role of the F protein in peste des petits ruminants virus (PPRV)-induced cytopathology, the F protein of PPRV was purified by immunoaffinity chromatography.
Devireddy, LR +3 more
openaire +3 more sources
Insights into protein sequencing with an α-Hemolysin nanopore by atomistic simulations [PDF]
Scientific Reports, 2019 AbstractSingle molecule protein sequencing would represent a disruptive burst in proteomic research with important biomedical impacts. Due to their success in DNA sequencing, nanopore based devices have been recently proposed as possible tools for the sequencing of peptide chains.
Di Muccio, Giovanni +4 more
openaire +4 more sources
Animals, 2021 A total of 318 nasal and rectal swabs were collected from 159 apparently healthy camels (Camelus dromedarius) randomly selected from five regions in southern and central Tunisia and screened for Staphylococcus aureus carriage. Staphylococcus spp.
Faten Ben Chehida +7 more
doaj +1 more source