Results 1 to 10 of about 6,571,241 (229)

Unfoldase-mediated protein translocation through an α-hemolysin nanopore [PDF]

Nature Biotechnology, 2013
Using nanopores to sequence biopolymers was proposed more than a decade ago. Recent advances in enzyme-based control of DNA translocation and in DNA nucleotide resolution using modified biological pores have satisfied two technical requirements of a functional nanopore DNA sequencing device. Nanopore sequencing of proteins was also envisioned. Although
Jeff, Nivala, Douglas B, Marks, Mark, Akeson   +2 more
openaire   +3 more sources

Staphylococcus aureus alpha-hemolysin activates the NLRP3-inflammasome in human and mouse monocytic cells. [PDF]

PLoS ONE, 2009
Community Acquired Methicillin Resistant Staphylococcus aureus (CA-MRSA) causes severe necrotizing infections of the skin, soft tissues, and lungs. Staphylococcal alpha-hemolysin is an essential virulence factor in mouse models of CA-MRSA necrotizing ...
Robin R Craven, Xi Gao, Irving C Allen, Denis Gris, Juliane Bubeck Wardenburg, Erin McElvania-Tekippe, Jenny P Ting, Joseph A Duncan   +7 more
doaj   +2 more sources

Targeted Regulation of Protein Expression in Vibrio parahaemolyticus [PDF]

Biology
V. parahaemolyticus has several virulence factors, including thermostable direct hemolysin (TDH), TDH-related hemolysin (TRH), and two separate type III secretion systems (T3SSs), T3SS1 and T3SS2.
Takashi Uebanso, Kei Kobayashi, Ayumi Masuda, Hitomi Iba, Mutsumi Aihara, Takaaki Shimohata, Kazuaki Mawatari, Akira Takahashi   +7 more
doaj   +2 more sources

Hemolysin Co-regulated Family Proteins Hcp1 and Hcp2 Contribute to Edwardsiella ictaluri Pathogenesis [PDF]

Frontiers in Veterinary Science, 2021
Edwardsiella ictaluri is a Gram-negative facultative intracellular pathogen causing enteric septicemia of catfish (ESC), a devastating disease resulting in significant economic losses in the U.S. catfish industry. Bacterial secretion systems are involved
Safak Kalindamar, Hossam Abdelhamed, Adef O. Kordon, Lesya M. Pinchuk, Attila Karsi   +4 more
doaj   +3 more sources

The UPEC Pore-Forming Toxin α-Hemolysin Triggers Proteolysis of Host Proteins to Disrupt Cell Adhesion, Inflammatory, and Survival Pathways

Cell Host and Microbe, 2012
SUMMARY Uropathogenic Escherichia coli (UPEC), which are the leading cause of both acute and chronic urinary tract infections, often secrete a labile pore-forming toxin known as α-hemolysin (HlyA).
Matthew Mulvey, Bijaya K Dhakal
exaly   +2 more sources

Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3)

Microbial Cell Factories, 2012
Background Extracellular expression of proteins has an absolute advantage in a large-scale industrial production. In our previous study, Thermobifida fusca cutinase, an enzyme mainly utilized in textile industry, was expressed via type II secretory ...
Su Lingqia, Chen Sheng, Yi Li, Woodard Ronald W, Chen Jian, Wu Jing   +5 more
doaj   +2 more sources

TolC, an Escherichia coli outer membrane protein required for hemolysin secretion. [PDF]

Proceedings of the National Academy of Sciences, 1990
Secretion of Escherichia coli alpha-hemolysin into the medium does not require the cleavage of an N-terminal signal peptide. The specific secretion apparatus was shown to consist of two proteins, HlyB and HlyD, both located in the inner membrane and encoded by genes contiguous to the hemolysin structural gene (hlyA).
C, Wandersman, P, Delepelaire
openaire   +3 more sources

Insights into the dynamic cell associated and secreted proteome of Staphylococcus aureus cultured in TSB and milk media: a proteomic analysis [PDF]

BMC Microbiology
The availability of nutrients is closely linked to the protein expression profile of microorganisms. In this study, a comparative investigation has been performed to examine the expression patterns of cell associated proteins and secreted proteins in ...
Wanting Zhu, Yujing Wang, Yanxin Li, Changjiang Zang, Hongning Jiang, Qijing Du, Jun Wang, Rongbo Fan, Rongwei Han, Yongxin Yang   +9 more
doaj   +2 more sources

Secretome analysis of Aspergillus fumigatus reveals Asp-hemolysin as a major secreted protein

International Journal of Medical Microbiology, 2011
Surface-associated and secreted proteins represent primarily exposed components of Aspergillus fumigatus during host infection. Several secreted proteins are known to be involved in defense mechanisms or immune evasion, thus, probably contributing to pathogenicity.
Dirk, Wartenberg, Katrin, Lapp, Ilse D, Jacobsen, Hans-Martin, Dahse, Olaf, Kniemeyer, Thorsten, Heinekamp, Axel A, Brakhage   +6 more
openaire   +3 more sources

Identification of two neutralizing human single-chain variable fragment antibodies targeting Staphylococcus aureus alpha-hemolysin [PDF]

Iranian Journal of Basic Medical Sciences, 2022
Objective(s): The inability of the host immune system to defeat Staphylococcus aureus is due to various secreted virulent factors such as leukocidins, superantigens, and hemolysins, which interrupt the function of immune components.
Somayeh Piri-Gavgani, Mostafa Ghanei, Abolfazl Fateh, Seyed Davar Siadat, Leila Nematollahi, Fatemeh Rahimi-Jamnani   +5 more
doaj   +1 more source