Results 171 to 180 of about 281,999 (311)

Advances and perspectives in animal models of human hepatitis A virus

Animal Models and Experimental Medicine, EarlyView.
Following HAV infection, humans, non‐human primates, and Ifnar1−/− mice develop characteristic manifestations of hepatitis A, including fecal viral shedding, elevated serum ALT levels, and inflammatory cell infiltration in the liver. In contrast, HAV‐infected human liver chimeric mice exhibit fecal viral shedding but do not develop clinical features of
Jian Li, Pei‐Yu Jiang, Hui Zhao, Cheng‐Feng Qin   +3 more
wiley   +1 more source

Bile acid retention in efferocytic macrophages shapes their inflammatory status during cholangitis. [PDF]

J Exp Med
Al Jawazneh A, Liebold I, Leyk S, Lanzloth C, Brackrock V, López-López T, Graute S, Aranda-Pardos I, Hamley M, Bahn J, Heeren J, Von Felden J, Lohse AW, Schramm C, Schwinge D, Sáez PJ, A-Gonzalez N, Huber S, Jacobs T, Adlung L, Worthmann A, Bosurgi L.   +21 more
europepmc   +1 more source

Metabolism of remimazolam in primary human hepatocytes during continuous long-term infusion in a 3-D bioreactor system

, 2019
Nora Freyer,1 Fanny Knöspel,1 Georg Damm,2 Selina Greuel,1 Christin Schneider,1 Daniel Seehofer,2 Thomas Stöhr,3 Karl-Uwe Petersen,3 Katrin Zeilinger1 1Berlin Brandenburg Center for Regenerative Therapies (BCRT), Charité &ndash ...
Freyer N, Stöhr T, Zeilinger K, Schneider C, Petersen KU, Greuel S, Damm G, Seehofer D, Knöspel F   +8 more
core  

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

The role of hepatocyte epigenetics in the pathogenesis of metabolic dysfunction-associated steatotic liver disease. [PDF]

Commun Med (Lond)
Zhu G, Zhao Q, Ran J, Zhang H, Yin S, Issa R, Shi J, Wu C, Li J.   +8 more
europepmc   +1 more source

Drug metabolism in fetal hepatocytes.

, 2019
The drug metabolising capability of the fetus has been investigated using freshly isolated and cultured fetal rat hepatocytes. Phase I metabolism has been investigated using 7-ethoxycoumarin and 7-ethoxyresorufin as substrates and Phase II using 7 ...
Worrell, Nan R.
core  

Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex

Advanced NanoBiomed Research, EarlyView.
We developed an assay to distinguish cellular binding from internalization. Compatible with microscopy and high‐throughput screening, the method identifies ligand‐mediated uptake. Applying top candidates to a protein‐based DNA carrier enhanced transfection efficiency, providing a rational strategy to improve non‐viral gene delivery systems.
David Scherer, Steffen Honrath, Jonas Kurmann, Alexander Klipp, Finn Brigger, Jean‐Christophe Leroux   +5 more
wiley   +1 more source

Contradictory Effects on Hepatocytes in ASMD. [PDF]

Int J Mol Sci
Sysoev M, Solovyov D, Shestopalov A, Kutsev S.   +3 more
europepmc   +1 more source

Toxicity of cadmium in hepatocytes.

, 1986
Freshly isolated hepatocytes from fed and starved rats were used as a model in the investigation of the mechanisms by which cadmium chloride exerts its toxic effects at the cellular level.
Ng, Jasmine Christina.
core  

SUCNR1 coordinates metabolic flux, mitochondrial function, and nutrient-dependent adaptation in hepatocytes. [PDF]

Sci Adv
Marsal-Beltran A, Salmerón-Pelado L, Ribas-Latre A, Repollés-de-Dalmau M, Rodríguez-Peña MM, Núñez-Roa C, Badia J, Madeira A, Novoa E, Beltrà M, Plata-Gómez AB, Capellades J, Escolà-Gil JC, Zorzano A, Yanes Ó, Efeyan A, Nogueiras R, Gracia-Sancho J, Vendrell J, Ceperuelo-Mallafré V, Fernández-Veledo S.   +20 more
europepmc   +1 more source