Results 161 to 170 of about 22,486 (204)

Compound 48/80 Activates Mast Cell Phospholipase D via Heterotrimeric GTP-Binding Proteins

The Journal of Pharmacology and Experimental Therapeutics, 2000
Previous studies have indicated the presence of a cholera toxin-sensitive phospholipase D (PLD) in cultured RBL-2H3 mast cells that is synergistically activated via calcium, protein kinase C, and another unidentified signal. Here we identify a third potential signal for activation transduced by a pertussis toxin-sensitive trimeric GTP-binding protein ...
A, Chahdi, P F, Fraundorfer, M A, Beaven
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Growth factor second messenger systems: Oncogenes and the heterotrimeric GTP‐;binding protein connection

Medicinal Research Reviews, 1993
We feel that there is now compelling evidence that the GTP-binding proteins play more than just a coordinating role in the actions of both tyrosine kinase and nontyrosine kinase receptor signal transduction. These similarities appear to represent just a small component of the convergence in the signaling pathways for structurally dissimilar receptor ...
M F, Crouch, I A, Hendry
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Cytosolic Localization of β3-Subunit of Heterotrimeric GTP Binding Protein in Rat Hearts

Biochemical and Biophysical Research Communications, 1995
The expression of three different subtypes of GTP binding protein beta-subunits (beta 1, beta 2 and beta 3) was examined in rat hearts at both mRNA and protein levels. Among three subtypes, beta 3 mRNA was more abundant in ventricles while beta 1 and beta 2 mRNAs were expressed ubiquitously among ventricles, atria and aortas.
S, Muramoto, T, Murakami, K, Urasawa, H, Kawaguchi, A, Kitabatake   +4 more
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Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins

2013
Heterotrimeric G-proteins are important signaling intermediates in all eukaryotes. These proteins link signal perception by a cell surface localized receptor to the downstream effectors of a given signaling pathways. The minimal core of the heterotrimeric G-protein complex consists of Gα, Gβ, and Gγ subunits, the G protein coupled receptor (GPCR) and ...
Swarup Roy, Choudhury, Corey S, Westfall, Dieter, Hackenberg, Sona, Pandey   +3 more
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Activation of Heterotrimeric GTP-Binding Proteins

1999
Guanosine triphosphate- (GTP-) binding proteins constitute a supergene family of proteins that utilize GTP binding and hydrolysis as a chemical switch. These proteins are activated by exchanging bound guanosine diphosphate (GDP) with GTP and are subsequently inactivated by hydrolyzing bound GTP to GDP.1–3 Hence, by shuttling between an inactive, GDP ...
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Purification of heterotrimeric GTP-binding proteins from brain: identification of a novel form of Go

Biochemistry, 1988
Using high-resolution Mono-Q anion-exchange chromatography, we purified four distinct GTP-binding proteins from bovine brain. Each consists of alpha and associated beta/gamma subunits, and each is a substrate for pertussis toxin catalyzed ADP-ribosylation.
P, Goldsmith, P S, Backlund, K, Rossiter, A, Carter, G, Milligan, C G, Unson, A, Spiegel   +6 more
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Heterotrimeric and small molecular mass GTP-binding proteins of rat brain neuronal and glial nuclei

Journal of Neuroscience Research, 1999
Heterotrimeric and small molecular mass guanine nucleotide binding (GTP-binding) proteins were found in neuronal and glial nuclei isolated from rat brain. Neuronal nuclei bound 0.213 +/- 0.055 pmoles of GTP/microg protein (n = 8) and glial nuclei bound 0.145 +/- 0.038 pmoles of GTP/microg protein (n = 8).
R P, Bhullar, D G, McCartney, J N, Kanfer   +2 more
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The Heterotrimeric GTP-Binding Protein, GS, Modulates The Cl−Conductance of Rat Parotid Acinar Secretory Granules

Biochemical and Biophysical Research Communications, 1997
Gsalpha has been reported to be present in rat parotid acinar secretory granule membrane (SGM) fractions. In the present study, we evaluated epitope orientation of Gsalpha on the secretory granule (SG) and the ability of Gs to modulate the Cl- conductance of isolated granules by measuring granule lysis.
E L, Watson, K T, Izutsu, K L, Jacobson, D H, Dijulio   +3 more
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Heterotrimeric GTP-Binding Proteins (G Proteins) and ADP-Ribosylation Factor (ARF) Regulate Priming of Endosomal Membranes for Fusion

Archives of Biochemistry and Biophysics, 1994
An in vitro assay that measures endosome fusion was used to characterize the role of guanosine triphosphate (GTP)-binding proteins in endocytosis. Guanosine 5',3-(thio)triphosphate (GTP gamma S), a nonhydrolyzable analog of GTP, stimulates the binding of cytosolic factors to the endosomal membrane (priming).
J M, Lenhard, M I, Colombo, P D, Stahl
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Albumin endocytosis is regulated by heterotrimeric GTP-binding protein G alpha i-3 in opossum kidney cells

American Journal of Physiology-Renal Physiology, 1996
Proteinuria is an adverse feature in patients with renal disease, possibly due to toxicity of albumin to proximal tubular cells. Albumin is reabsorbed from tubular fluid by receptor-mediated endocytosis. The mechanism of regulation of the endocytosis is unknown. The large quantities of G proteins in proximal tubular cell apical membranes suggests that
N J, Brunskill, N, Cockcroft, S, Nahorski, J, Walls   +3 more
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