Results 41 to 50 of about 2,645,999 (191)

Affinity labeling of GTP-binding proteins in cellular extracts [PDF]

, 1992
GTP-binding proteins in cellular extracts from Escherichia coli, Thermus thermophilus, yeast, wheat germ or calf thymus were identified using in situ periodate-oxidized [α-32P]GTP as affinity label.
Faulhammer, Heinz G, Löw, Andreas, Andreas Löw, Sprinzl, Mathias, Heinz G Faulhammer, Mathias Sprinzl   +5 more
core   +1 more source

Sodium Ions Affect GPR17 Conformational States and Functionality

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Sodium is an allosteric modulator of several class‐A GPCR regulating active versus inactive conformation state and may be a critical issue for the optimization of crystallization procedures. Herein, the role of Na+ in the regulation of GPR17 conformational states was studied through MD simulations of GPR17 wild‐type and mutated on two ...
Luca Palazzolo, Simona Daniele, Davide Bianchi, Lara Russo, Stefano Capaldi, Maria Letizia Trincavelli, Ivano Eberini   +6 more
wiley   +1 more source

Interaction of mastoparan with the low molecular mass GTP-binding proteins rho/rac [PDF]

, 1991
Mastoparan, which has been shown to active G proteins [1], inhibits the ADP-ribosylation of 20 kDa human platelet membrane proteins catalyzed by Clostridium botulinum exoenzyme C3 half-maximally and maximally (90%) at 20 and 100 μM concentrations ...
Klaus Aktories, Haberman, Barbara, Christiane Mohr, Mohr, Christiane, Barbara Haberman, Gertrud Koch, Ingo Just, Just, Ingo, Aktories, Klaus, Koch, Gertrud   +9 more
core   +1 more source

Compartmentalisation in cAMP signalling: A phase separation perspective

British Journal of Pharmacology, EarlyView.
Cells rely on precise spatiotemporal control of signalling pathways to ensure functional specificity. The compartmentalisation of cyclic AMP (cAMP) and protein kinase A (PKA) signalling enables distinct cellular responses within a crowded cytoplasmic space.
Milda Folkmanaite, Manuela Zaccolo
wiley   +1 more source

The potential for biased signalling in the P2Y receptor family of GPCRs

British Journal of Pharmacology, EarlyView.
The purinergic receptor family is primarily activated by nucleotides, and contains members of both the G protein coupled‐receptor (GPCR) superfamily (P1 and P2Y) and ligand‐gated ion channels (P2X). The P2Y receptors are widely expressed in the human body, and given the ubiquitous nature of nucleotides, purinergic signalling is involved with a plethora
Claudia M. Sisk, Simon Pitchford, Graham Ladds   +2 more
wiley   +1 more source

Regulators of G protein signalling proteins in the human myometrium [PDF]

, 2009
The contractile state of the human myometrium is controlled by extracellular signals that promote relaxation or contraction. Many of these signals function through G proteincoupled receptors at the cell surface, stimulating heterotrimeric G proteins and ...
Ladds, Graham, Thornton, Steven, Zervou, Sevasti I., Vatish, Manu, Davey, John   +4 more
core   +1 more source

Adenylyl cyclase 9 as a molecular scaffold to dissect the mechanisms of membrane adenylyl cyclases

Protein Science, Volume 35, Issue 10, October 2026.
Abstract Adenylyl cyclases (ACs) convert ATP into the second messenger cAMP, thus directly influencing cellular signaling in response to a wide variety of stimuli. Despite their physiological importance, structural studies of isoform‐specific AC regulation are compounded by difficulties in AC expression and purification.
Ilayda Kantarci, Haoriwa Haoriwa, Volodymyr M. Korkhov   +2 more
wiley   +1 more source

Influence of expression and purification protocols on Gα biochemical activity: kinetics of plant and mammalian G protein cycles

Frontiers in Molecular Biosciences
Heterotrimeric G proteins, composed of Gα, Gβ, and Gγ subunits, are a class of signal transduction complexes with broad roles in human health and agriculturally relevant plant physiological and developmental traits.
Timothy E. Gookin, David Chakravorty, Sarah M. Assmann   +2 more
doaj   +1 more source

Rabaptin5 is recruited to endosomes by Rab4a and Rabex5 to regulate endosome maturation [PDF]

, 2014
Membrane trafficking between organelles is fundamental to the existence of eukaryotic cells. A multitude of proteins is involved in membrane trafficking, acting as building blocks for transport carriers, regulators of transport, and targeting and fusion ...
Kälin, Simone
core   +1 more source

Conditional eIF2A Deletion Suggests Extra‐Adipose Mechanisms Underlying Metabolic Syndrome in Total‐Body eIF2A Knockout Mice

FASEB BioAdvances, Volume 8, Issue 9, September 2026.
Adipose tissue‐specific deficiency of eIF2A is insufficient to reproduce the metabolic defects observed in total‐body eIF2A knockout mice, suggesting that the systemic metabolic effects observed in these mice may arise from coordinated functions across multiple organs.
Adedeji Isaac Adeloye, Richard Anderson, Anchal Agarwal, Barsanjit Mazumder, Taras Y. Nazarko, Atefeh Bagheri, Archana Prabahar, Peng Jiang, Frank Tedeschi, William C. Merrick, David A. Buchner, Maria Hatzoglou, Roman V. Kondratov, Anton A. Komar   +13 more
wiley   +1 more source