Results 31 to 40 of about 273,042 (329)

Targeted Amplicon Sequencing for Single-Nucleotide-Polymorphism Genotyping of Attaching and Effacing Escherichia coli O26:H11 Cattle Strains via a High-Throughput Library Preparation Technique [PDF]

Applied and Environmental Microbiology, 2015
ABSTRACT Enterohemorrhagic Escherichia coli (EHEC) O26:H11, a serotype within Shiga toxin-producing E. coli (STEC) that causes severe human disease, has been considered to have evolved from attaching and effacing E. coli (AEEC) O26:H11 through the acquisition
Sarah A. Ison, Sabine Delannoy, Marie Bugarel, T. G. Nagaraja, David G. Renter, Henk C. den Bakker, Kendra K. Nightingale, Patrick Fach, Guy H. Loneragan   +8 more
openalex   +4 more sources

Optimization of conditions for in vitro modeling of subgingival normobiosis and dysbiosis

Frontiers in Microbiology, 2022
Modeling subgingival microbiome in health and disease is key to identifying the drivers of dysbiosis and to studying microbiome modulation. Here, we optimize growth conditions of our previously described in vitro subgingival microbiome model. Subgingival
Divyashri Baraniya, Thuy Do, Tsute Chen, Jasim M. Albandar, Susan M. Chialastri, Deirdre A. Devine, Philip D. Marsh, Nezar N. Al-Hebshi   +7 more
doaj   +1 more source

MEGARes and AMR++, v3.0: an updated comprehensive database of antimicrobial resistance determinants and an improved software pipeline for classification using high-throughput sequencing

Nucleic Acids Res., 2022
Antimicrobial resistance (AMR) is considered a critical threat to public health, and genomic/metagenomic investigations featuring high-throughput analysis of sequence data are increasingly common and important.
N. Bonin, E. Doster, Hannah Worley, L. Pinnell, Jonathan E. Bravo, Peter M Ferm, S. Marini, M. Prosperi, N. Noyes, P. Morley, C. Boucher   +10 more
semanticscholar   +1 more source

Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: applied to turkey [PDF]

BMC Genomics, 2009
AbstractBackgroundThe development of second generation sequencing methods has enabled large scale DNA variation studies at moderate cost. For the high throughput discovery of single nucleotide polymorphisms (SNPs) in species lacking a sequenced reference genome, we set-up an analysis pipeline based on a short read de novo sequence assembler and a ...
Hindrik H. D. Kerstens, R.P.M.A. Crooijmans, A. Veenendaal, Bert Dibbits, Thomas F. C. Chin‐A‐Woeng, Johan T. den Dunnen, Martien A. M. Groenen   +6 more
openalex   +6 more sources

Search for Novel Mutational Targets in Human Endocrine Diseases [PDF]

Endocrinology and Metabolism, 2019
The identification of disease-causing genetic variations is an important goal in the field of genetics. Advancements in genetic technology have changed scientific knowledge and made it possible to determine the basic mechanism and pathogenesis of human ...
So Young Park, Myeong Han Seo, Sihoon Lee   +2 more
doaj   +1 more source

Clinical application of high throughput molecular screening techniques for pharmacogenomics. [PDF]

, 2011
Genetic analysis is one of the fastest-growing areas of clinical diagnostics. Fortunately, as our knowledge of clinically relevant genetic variants rapidly expands, so does our ability to detect these variants in patient samples.
Schrijver, Iris, Wiita, Arun P
core   +1 more source

High-throughput Interpretation of Killer-cell Immunoglobulin-like Receptor Short-read Sequencing Data with PING

bioRxiv, 2021
The killer-cell immunoglobulin-like receptor (KIR) complex on chromosome 19 encodes receptors that modulate the activity of natural killer cells, and variation in these genes has been linked to infectious and autoimmune disease, as well as having bearing
Wesley M. Marin, R. Dandekar, D. Augusto, T. Yusufali, B. Heyn, J. Hofmann, V. Lange, J. Sauter, P. Norman, J. Hollenbach   +9 more
semanticscholar   +1 more source

Bacteriome analysis of Aggregatibacter actinomycetemcomitans-JP2 genotype-associated Grade C periodontitis in Moroccan adolescents

Frontiers in Oral Health, 2023
BackgroundGrade C (previously aggressive) periodontitis (GCP) in adolescents is prevalent in certain parts of Africa where it is associated with JP2 genotype, a highly virulent strain of Aggregatibacter actinomycetemcomitans. The aim of this study was to
Vijaya Lakshmi Pavani Molli, Jamila Kissa, Divyashri Baraniya, Amina Gharibi, Tsute Chen, Nezar N. Al-Hebshi, Jasim M. Albandar   +6 more
doaj   +1 more source

Aptardi predicts polyadenylation sites in sample-specific transcriptomes using high-throughput RNA sequencing and DNA sequence

Nature Communications, 2021
Annotation of polyadenylation sites from short-read RNA sequencing alone is a challenging computational task. Other algorithms rooted in DNA sequence predict potential polyadenylation sites; however, in vivo expression of a particular site varies based ...
Ryan Lusk, Evan Stene, F. Banaei-Kashani, B. Tabakoff, K. Kechris, L. Saba   +5 more
semanticscholar   +1 more source

Cordblood-Based High-Throughput Screening for Deafness Gene of 646 Newborns in Jinan Area of China [PDF]

Clinical and Experimental Otorhinolaryngology, 2015
ObjectivesInfants with slight/mild or late-onset hearing impairment might be missed in universal newborn hearing screening (UNHS). We identified the mutation hot spot of common deaf gene in the newborns in Jinan area population by screening the mutation ...
Shou-Xia Li, Ding-Li Chen, Su-Bin Zhao, Li-Li Guo, Hai-Qin Feng, Xiao-Fang Zhang, Li-Li Ping, Zhi-Ming Yang, Cai-Xia Sun, Gen-Dong Yao   +9 more
doaj   +1 more source