Results 61 to 70 of about 271,712 (303)

Chromosomal analyses of human giant diploid oocytes by next‐generation sequencing

Reproductive Medicine and Biology, 2021
Purpose Although giant oocytes (GOs) having about twice cytoplasmic volume compared with general oocytes in mammals including the human are rarely recovered, it is thought that GOs have potentially chromosomal abnormalities.
Hiroomi Kawano, Naoki Yamashita, Junya Ito, Naomi Kashiwazaki   +3 more
doaj   +1 more source

BamView: visualizing and interpretation of next-generation sequencing read alignments. [PDF]

, 2012
So-called next-generation sequencing (NGS) has provided the ability to sequence on a massive scale at low cost, enabling biologists to perform powerful experiments and gain insight into biological processes.
Berriman, Matthew, Carver, Tim, Harris, Simon R., McQuillan, Jacqueline A., Otto, Thomas D., Parkhill, Julian   +5 more
core   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Clustering and Alignment of Polymorphic Sequences for HLA-DRB1 Genotyping [PDF]

, 2013
Located on Chromosome 6p21, classical human leukocyte antigen genes are highly polymorphic. HLA alleles associate with a variety of phenotypes, such as narcolepsy, autoimmunity, as well as immunologic response to infectious disease.
A McKenna, A van Dijk, AA Rossini, AK Lancaster, C Gabriel, C Karanes, C Lind, C Wang, CE Voorter, CL Holcomb, G Bentley, Gaia Bellone, Gualtiero Colombo, H Erlich, J Ng, J Richards, J Robinson, JM Rothberg, JP Achkar, Kathryn Roeder, KK Ballen, L Becquemont, L Breiman, M Hamady, M Maiers, Massimo Trucco, RL Erlich, S Kurtz, S Lafon, SA Dunbar, SM Lank, SM Lank, Steven Ringquist, T Shiina, T Shiina, Ying Lu   +35 more
core   +7 more sources

Next-Generation Sequencing Reveals Differentially Expressed Genes and Pathways in Urethral Cancer: Exploring A Poorly Understood Malignancy [PDF]

Journal of Urologic Oncology
Purpose Primary urethral cancer (PUC) is an uncommon malignancy with scarce diagnostic and treatment options, resulting in a limited understanding of its genetic foundation. This exploratory study compares gene expression profiles between urethral cancer
Nickolas Kinachtchouk, Samantha Freeman, Rachael Gotlieb, Kailey Hooper, Travis Sullivan, Eric Burks, Kimberly Christ, Alex Vanni   +7 more
doaj   +1 more source

Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme. [PDF]

, 2020
Massively parallel, quantitative measurements of biomolecular activity across sequence space can greatly expand our understanding of RNA sequence-function relationships. We report the development of an RNA-array assay to perform such measurements and its
Andreasson, Johan OL, Block, Steven M, Greenleaf, William J, Savinov, Andrew   +3 more
core  

A novel cost effective and high-throughput isolation and identification method for marine microalgae [PDF]

, 2014
Background: Marine microalgae are of major ecologic and emerging economic importance. Biotechnological screening schemes of microalgae for specific traits and laboratory experiments to advance our knowledge on algal biology and evolution strongly benefit
Jahn, Martin T, Mock, Thomas, Schmidt, Katrin   +2 more
core   +2 more sources

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Multidimensional analysis of the host response reveals prognostic and pathogen-driven immune subtypes among adults with sepsis in Uganda

Critical Care, 2022
Background The global burden of sepsis is concentrated in sub-Saharan Africa, where severe infections disproportionately affect young, HIV-infected adults and high-burden pathogens are unique. In this context, poor understanding of sepsis immunopathology
Matthew J. Cummings, Barnabas Bakamutumaho, Adam Price, Nicholas Owor, John Kayiwa, Joyce Namulondo, Timothy Byaruhanga, Moses Muwanga, Christopher Nsereko, Stephen Sameroff, Rafal Tokarz, Wai Wong, Shivang S. Shah, Michelle H. Larsen, W. Ian Lipkin, Julius J. Lutwama, Max R. O’Donnell   +16 more
doaj   +1 more source

Detection of Persistent Viruses by High-Throughput Sequencing in Tomato and Pepper from Panama: Phylogenetic and Evolutionary Studies

Plants, 2021
High-throughput sequencing from symptomatic tomato and pepper plants collected in Panama rendered the complete genome of the southern tomato virus (isolate STV_Panama) and bell pepper endornavirus (isolate BPEV_Panama), and almost-complete genomes of ...
Luis Galipienso, Laura Elvira-González, Leonardo Velasco, José Ángel Herrera-Vásquez, Luis Rubio   +4 more
doaj   +1 more source