Results 121 to 130 of about 1,236,846 (314)

Peptide-encoding mRNA barcodes for the high-throughput in vivo screening of libraries of lipid nanoparticles for mRNA delivery

Nature Biomedical Engineering, 2023
Luke H. Rhym, R. Manan, Antonius Koller, Georgina Stephanie, Daniel G. Anderson   +4 more
semanticscholar   +1 more source

Enzymatic degradation of biopolymers in amorphous and molten states: mechanisms and applications

FEBS Open Bio, EarlyView.
This review explains how polymer morphology and thermal state shape enzymatic degradation pathways, comparing amorphous and molten biopolymer structures. By integrating structure–reactivity principles with insights from thermodynamics and enzyme engineering, it highlights mechanisms that enable efficient polymer breakdown.
Anđela Pustak, Aleksandra Maršavelski
wiley   +1 more source

High throughput screening

High Throughput Screening (HTS) is a drug discovery process that allows automated detection of large numbers of chemical and/or biological compounds for a specific biological target. It speed up target analysis, as large scale compound libraries can be quickly screened in a cost-effective way.
Nuwan Perera, Rangika S. Hikkaduwa Koralege   +1 more
  +4 more sources

Structure-guided discovery of novel dUTPase inhibitors with anti-Nocardia activity by computational design

Journal of Enzyme Inhibition and Medicinal Chemistry
The zoonosis caused by Nocardia is increasing seriously. But commonly used antibiotic drugs often lead to resistance. N. seriolae dUTPase (NsdUTPase) plays a key role in the proliferation of Nocardia, and was regarded as a potent drug target.
Zhi-Zheng Wang, Jun Weng, Jing Qi, Xin-Xin Fu, Ban-Bin Xing, Yang Hu, Chun-Hsiang Huang, Chin-Yu Chen, Zigong Wei   +8 more
doaj   +1 more source

Dihydroorotate dehydrogenase (DHODH) regulates trophoblast syncytialization through organelle stress–induced cellular senescence

FEBS Open Bio, EarlyView.
The inhibition of mitochondrial dihydroorotate dehydrogenase (DHODH) impairs syncytialization and induces cellular senescence via mitochondrial and endoplasmic reticulum stress in human trophoblast stem cells, elevating sFlt1/PlGF levels, a hallmark of placental dysfunction in hypertensive disorders of pregnancy.
Kanoko Yoshida, Kazuya Kusama, Ayaka Horiuchi, Yu Kawaguchi, Atsuya Tsuru, Mikihiro Yoshie, Kazuhiro Tamura   +6 more
wiley   +1 more source

A Bayesian measurement error model for two-channel cell-based RNAi data with replicates

, 2012
RNA interference (RNAi) is an endogenous cellular process in which small double-stranded RNAs lead to the destruction of mRNAs with complementary nucleoside sequence. With the production of RNAi libraries, large-scale RNAi screening in human cells can be
Chang, I-Shou, Chen, Chih-Yu, Chen, Chung-Hsing, Hsiung, Chao A., Huang, Jing-Ying, Jeng, King-Song, Su, Wen-Chi, Tsai, Fang-Yu, Wang, Wen-Chang   +8 more
core   +1 more source

Whole‑exome evolutionary profiling of osteosarcoma uncovers metastasis‑related driver mutations and generates an independently validated predictive classifier

Journal of Translational Medicine
Background Osteosarcoma is the most common primary malignant bone tumor, with high invasiveness and metastatic potential and a poor prognosis in patients with metastatic cancer. Despite the rapid advancements in genomics in recent years that provided new
Zhen Wang, Zhe Wang, Ruoyu Wang, Zumin Wang, Xiangyang Cao, Rui Chen, Zebing Ma, Shanshan Liang, Shuai Tao   +8 more
doaj   +1 more source

Novel PHL derivatives targeting quorum sensing: A strategy to combat Pseudomonas aeruginosa infections

Biofilm
Pseudomonas aeruginosa (P. aeruginosa) is a common pathogenic bacterium that is widely distributed and highly pathogenic. Its pathogenicity is closely related to the formation of biofilms and virulence factors.
Juanyan Liu, Yi Deng, Xinran Wang, Piao Luo, Shuaiwen Ding, Weizhong Zhang, Shahab Uddin, Hongyu Li, Yang Li   +8 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source