Results 141 to 150 of about 16,617,738 (336)

GAD65 Antibody ELISA With Extended Reportable Range: Validation and Guidance for Neurological Practice

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To (1) validate GAD65‐ELISA detection and quantification for type 1 diabetes mellitus and autoimmune neurological diagnoses, (2) correlate ELISA results (reference range < 5 IU/mL) with established radioimmunoprecipitation assay (RIA; ≤ 0.02 nmol/L), and (3) define ELISA clinical utility and pitfalls.
Andrew McKeon, Dana Olofson, Diana Anissian, Divyanshu Dubey, Eoin P. Flanagan, Max C. Herman, Sarosh R. Irani, Daniel H. Lachance, Maria A. Willrich, Sean Pittock, John R. Mills, Anastasia Zekeridou   +11 more
wiley   +1 more source

High Throughput Screening

High Throughput Screening (HTS) is a drug discovery process that allows automated detection of large numbers of chemical and/or biological compounds for a specific biological target. It speed up target analysis, as large scale compound libraries can be quickly screened in a cost-effective way.
Nuwan Perera, Rangika S. Hikkaduwa Koralege   +1 more
openaire   +3 more sources

A CRISPR platform for targeted in vivo screens identifies Toxoplasma gondii virulence factors in mice

Nature Communications, 2019
Targeted CRISPR libraries expand the use of genetic screens across experimental conditions. Here, the authors develop a method for generating and analysing small scale custom CRISPR libraries and use it in the human and livestock pathogen Toxoplasma ...
Joanna Young, Caia Dominicus, Jeanette Wagener, Simon Butterworth, Xingda Ye, Gavin Kelly, Merav Ordan, Becky Saunders, Rachael Instrell, Michael Howell, Aengus Stewart, Moritz Treeck   +11 more
doaj   +1 more source

Preprint: An automated high-throughput system for phenotypic screening of chemical libraries on C. elegans and parasitic nematodes

, 2017
Parasitic nematodes infect hundreds of millions of people and farmed livestock. Further, plant parasitic nematodes result in major crop damage. The pipeline of therapeutic compounds is limited and parasite resistance to the existing anthelmintic ...
Lomas, D.A., Matthews, J.B., Else, K.J., Morrison, A.A., Brown, A.E., Forman, R., Buckingham, S.D., Wynne, G.M., Partridge, F.A., Sattelle, D.B., Willis, N.J., Russell, A.J.   +11 more
core   +1 more source

Re‐Purposing Sapropterin (Kuvan) for ACTA2‐Related Multisystemic Smooth Muscle Dysfunction Syndrome: A Translational Mechanistic and First‐In‐Human Therapeutic Report

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Multisystemic smooth muscle dysfunction syndrome (MSMDS) is an ultra‐rare, ACTA2‐related disorder characterized by severe cerebrovascular disease, aortic aneurysms, and smooth muscle dysfunction. Using molecular dynamics simulations and in silico drug screening, we identified that sapropterin dihydrochloride (Kuvan) is a candidate capable of ...
Moran Hausman‐Kedem, Noa Bielopolski, Vijai Krishnan, Shane Wald‐Altman, Shelly I Shrian, Omer Bar‐Yosef, Livia Kapusta, Claire Shamber, Patricia L. Musolino, Miguel Weil   +9 more
wiley   +1 more source

Catalyst Optimization in Olefin Polymerization using an HTE/QSAR workflow: Intuitive 3D-Steric Descriptors to Tackle the Database Size Problem

, 2021
Catalyst screening in olefin polymerization is an inherently complex problem. Even using high-throughput experimentation (HTE) tools, databases are usually tiny (at least when compared to those in pharma), rarely comprised of more than several dozen ...
Roberta Cipullo, Georgy P. Goryunov, Peter H. M. Budzelaar, Dmitry V. Uborsky, Vyatcheslav V. Izmer, Antonio Vittoria, Christian Ehm, Mikhail I. Sharikov, Pavel S. Kulyabin, Vincenzo Busico, Alexander Z. Voskoboynikov   +10 more
core  

Augmenting and Assaying Nav1.1 Protein Quantity for Dravet Syndrome Therapy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Dravet Syndrome (DS) is a developmental and epileptic encephalopathy predominantly caused by heterozygous loss‐of‐function variants in SCN1A, which encodes Nav1.1. Conserved upstream open reading frames (uORFs) in SCN1A were validated to regulate translation in reporter assays, demonstrating the therapeutic viability of increasing Nav1.1 from ...
Aiswarya Saravanan, Jordan Safran, Anna Loughman, Grace Lin, Shivanshi Vaid, Dana Allababidi, Peter M. Tessier, Louis T. Dang   +7 more
wiley   +1 more source

Schisandrin B Exerts Radiosensitizing Effects on Breast Cancer via Dual Mechanisms of Cell Cycle/DNA Repair and Gut Microbiota-Immune Axis Modulation

Pharmaceuticals
Background/Objectives: Schisandrin B (Sch B), a bioactive lignan of Schisandra chinensis has been commonly investigated for its antitumor activities, yet its radiosensitizing effect and mechanism remain unclear.
Yanhua Fang, Mengxuan Wang, Man Tong, Yue Wang, Zeshuo Feng, Ruoyu Wang, Zhe Wang, Lingyun Jia, Shanshan Liang   +8 more
doaj   +1 more source

High-throughput screening with transcriptomics [PDF]

Nature Methods, 2020
openaire   +2 more sources

Microarray technology as a universal tool for high-throughput analysis of biological systems

, 2006
Over the last years microarray technology has become one of the principal platform technologies for the high-throughput analysis of biological systems.
Jacob, A, Hoheisel, J D, Bartscherer, K, Sobek, J, Angenendt, P   +4 more
core   +1 more source