Results 1 to 10 of about 1,486 (194)
New High-Throughput Screening Assays for Biocatalysis
CHIMIA, 2001 High-throughput screening for catalysis is a critical technology in all experiments aimed at modifying or creating enzymes by directed evolution, as well as for biodiversity mining for new catalysts.
Jean-Louis Reymond
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Spectrophotometric Enzyme Assays for High-Throughput Screening
Food Technology and Biotechnology, 2004 This paper reviews high-throughput screening enzyme assays developed in our laboratory over the last ten years. These enzyme assays were initially developed for the purpose of discovering catalytic antibodies by screening cell culture supernatants, but ...
Jean-Louis Reymond
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Apoptosis assessment in high-content and high-throughput screening assays
BioTechniques, 2021 Here the authors describe the development of AUTOptosis, an economical and rapid apoptosis monitoring method suitable for high-content and high-throughput screening assays.
Céline Rens +5 more
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siMacro: A Fast and Easy Data Processing Tool for Cell-Based Genomewide siRNA Screens [PDF]
Genomics & Informatics, 2013 Growing numbers of studies employ cell line-based systematic short interfering RNA (siRNA) screens to study gene functions and to identify drug targets.
Nitin Kumar Singh +4 more
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FLICK: An optimized plate reader-based assay to infer cell death kinetics
STAR Protocols, 2021 Summary: Evaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity.
Ryan Richards +2 more
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A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
STAR Protocols, 2022 Summary: Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with ...
Benjamin Louis Sievers +2 more
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Screening and High-Throughput Platelet Assays [PDF]
, 2018 High-throughput assays are important biological research tools but are rarely utilized for platelet research. However, screening compounds for efficacy against a physiologically relevant cellular response in primary cells such as platelets can be an advantageous approach to compound screening and drug development. In this section we describe a panel of
Bye, Alexander P +2 more
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High-Throughput Screening to Predict Chemical-Assay Interference [PDF]
Scientific Reports, 2020 AbstractThe U.S. federal consortium on toxicology in the 21st century (Tox21) produces quantitative, high-throughput screening (HTS) data on thousands of chemicals across a wide range of assays covering critical biological targets and cellular pathways.
Alexandre Borrel +8 more
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Scientific Reports, 2023 The large unmet demand for new heart failure therapeutics is widely acknowledged. Over the last decades the contractile myofilaments themselves have emerged as an attractive target for the development of new therapeutics for both systolic and diastolic ...
Priyanka Parijat +6 more
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STAR Protocols, 2021 Summary: Autophagy measurement has been challenging due to the transient nature of autophagy vesicles, in which degradation of cargo occurs. Here, we present a protocol to monitor starvation-induced autophagy using a live high-throughput microscopy ...
Ada Nowosad, Arnaud Besson
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