Discovery of novel cardiac troponin activators using fluorescence polarization-based high throughput screening assays [PDF]
Scientific Reports, 2023 The large unmet demand for new heart failure therapeutics is widely acknowledged. Over the last decades the contractile myofilaments themselves have emerged as an attractive target for the development of new therapeutics for both systolic and diastolic ...
Priyanka Parijat +6 more
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High-yield synthesis and purification of recombinant human GABA transaminase for high-throughput screening assays [PDF]
Journal of Enzyme Inhibition and Medicinal Chemistry, 2021 Many studies have focussed on modulating the activity of γ-aminobutyric acid transaminase (GABA-T), a GABA-catabolizing enzyme, for treating neurological diseases, such as epilepsy and drug addiction. Nevertheless, human GABA-T synthesis and purification
Mingu Gordon Park +5 more
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Mining Chemical Activity Status from High-Throughput Screening Assays. [PDF]
PLoS ONE, 2015 High-throughput screening (HTS) experiments provide a valuable resource that reports biological activity of numerous chemical compounds relative to their molecular targets.
Othman Soufan +6 more
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Characterizing chlorotriazine effects in cancer-relevant high-throughput screening assays [PDF]
Frontiers in ToxicologyIntroductionHigh-throughput screening (HTS) in vitro testing can be a powerful tool for evaluating chemicals across an abundance of mechanistic, targeted assay systems.
Agnes L. Karmaus, Alex Charlton
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Stress-Based High-Throughput Screening Assays to Identify Inhibitors of Cell Envelope Biogenesis [PDF]
Antibiotics, 2020 The structural integrity of the Gram-negative cell envelope is guarded by several stress responses, such as the σE, Cpx and Rcs systems. Here, we report on assays that monitor these responses in E.
Maurice Steenhuis +3 more
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Integrating AUROC and SSMD for quality control in high-throughput screening assays [PDF]
SLAS DiscoveryHigh-throughput screening (HTS) assays are pivotal in modern biomedical research, particularly in drug discovery and functional genomics. Ensuring the quality and reliability of HTS data is critical, especially when dealing with the small sample sizes ...
Xiaohua Douglas Zhang
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Bioactivity Signatures of Drugs vs. Environmental Chemicals Revealed by Tox21 High-Throughput Screening Assays [PDF]
Frontiers in Big Data, 2019 Humans are exposed to tens of thousands of chemicals over the course of a lifetime, yet there remains inadequate data on the potential harmful effects of these substances on human health.
Deborah K. Ngan +6 more
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High-throughput screening assays to identify small molecules preventing photoreceptor degeneration caused by the rhodopsin P23H mutation. [PDF]
Methods Mol Biol, 2015 Chen Y, Tang H.
europepmc +2 more sources
Interference of metal ions on the bioluminescent signal of firefly, Renilla, and NanoLuc luciferases in high-throughput screening assays. [PDF]
Front ChemCanyelles I Font F +4 more
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Apoptosis assessment in high-content and high-throughput screening assays
BioTechniques, 2021 Here the authors describe the development of AUTOptosis, an economical and rapid apoptosis monitoring method suitable for high-content and high-throughput screening assays.
Céline Rens +5 more
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