Results 91 to 100 of about 229,438 (321)

High Throughput Screening Assays for Bacterial Primases

The FASEB Journal, 2006
Bacterial primase is a DNA‐dependent RNA polymerase that functions at the replication fork to create short oligoribonucleotide polymers. Knockout and conditional mutant experiments have indicated that primase is an essential replication protein in three different bacteria.
Mark A. Griep, Scott A. Koepsell, Steven H. Hinrichs   +2 more
openaire   +1 more source

Perspectives in educating molecular pathologists on liquid biopsy: Toward integrative, equitable, and decentralized precision oncology

Molecular Oncology, EarlyView.
Liquid biopsy enables minimally invasive, real‐time molecular profiling through analysis of circulating biomarkers in biological fluids. This Perspective highlights the importance of training pathologists through integrative educational programs, such as the European Masters in Molecular Pathology, to ensure effective and equitable implementation of ...
Marius Ilié, Umberto Malapelle, Catherine Alix‐Panabières, Claus Lindbjerg Andersen, Sandra Chlebowski, Vivien Lake, Caroline Lacoux, Virginie Lespinet‐Fabre, Olivier Bordone, Simon Heeke, Christophe Bontoux, Ellen Heitzer, Klaus Pantel, Paul Hofman   +13 more
wiley   +1 more source

In Vitro Screening Test Using Leishmania Promastigotes Stably Expressing mCherry Protein [PDF]

, 2014
Transgenic Leishmania major and Leishmania donovani axenic promastigotes constitutively expressing mCherry were used for in vitro antileishmanial drug screening. This method requires minimal sample manipulation and can be easily adapted to automatic drug
Miguel A. Morales, Vacchina, Paola
core   +2 more sources

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source

POLARISED views and FRETting about probe modulation assays: Learning from High Throughput Screening

SLAS Discovery
Fluorescent probe modulation assays are a widely used approach to monitor displacement or stabilisation of fluorescently labelled tool ligands by test compounds.
Alice Lanne, Catherine Bardelle, Gareth Davies, Antonia Turberville, Hannah Semple, Rachel Moore, Geoffrey A. Holdgate   +6 more
doaj   +1 more source

Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans [PDF]

, 2018
Background: Advances in automated image-based microscopy platforms coupled with high-throughput liquid workflows have facilitated the design of large-scale screens utilising multicellular model organisms such as Caenorhabditis elegans to identify genetic
Artal Sanz, Marta, Askjaer, Peter, Baumeister, Ralf, Erinjeri, Annmary Paul, González Hernández, Sara, Hernando Rodríguez, Blanca, Millar, Val, Muñoz, Manuel J., Olmedo López, María, Rodríguez Palero, María Jesús, Schulze, Bettina   +10 more
core   +1 more source

Next‐generation proteomics improves lung cancer risk prediction

Molecular Oncology, EarlyView.
This is one of very few studies that used prediagnostic blood samples from participants of two large population‐based cohorts. We identified, evaluated, and validated an innovative protein marker model that outperformed an established risk prediction model and criteria employed by low‐dose computed tomography in lung cancer screening trials.
Megha Bhardwaj, Clara Frick, Ben Schöttker, Bernd Holleczek, Hermann Brenner   +4 more
wiley   +1 more source

Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology

STAR Protocols
Summary: Comprehensive characterization of small-molecule degraders, including binary and ternary complex formation and degradation efficiency, is critical for bifunctional ligand development and understanding structure-activity relationships.
N. Connor Payne, Saki Ichikawa, Christina M. Woo, Ralph Mazitschek   +3 more
doaj   +1 more source

Evaluation of TypeSeq, a Novel High-Throughput, Low-Cost, Next-Generation Sequencing-Based Assay for Detection of 51 Human Papillomavirus Genotypes. [PDF]

, 2019
BackgroundHuman papillomaviruses (HPV) cause over 500 000 cervical cancers each year, most of which occur in low-resource settings. Human papillomavirus genotyping is important to study natural history and vaccine efficacy. We evaluated TypeSeq, a novel,
Boland, Joseph, Cortes, Bernal, Cullen, Michael, CVT Group, Dunn, S Terence, Herrero, Rolando, Hildesheim, Allan, Kreimer, Aimée R, Mirabello, Lisa, Porras, Carolina, Quint, Wim, Roberson, David, Rodriguez, Ana Cecilia, Sampson, Joshua, Schiffman, Mark, Van Doorn, Leen-Jan, Wagner, Sarah, Walker, Joan, Wentzensen, Nicolas, Yeager, Meredith, Zuna, Rosemary   +20 more
core   +1 more source

High‐Throughput Miniaturized Screening of Nanoparticle Formation via Inkjet Printing [PDF]

, 2018
This is the peer reviewed version of the following article:Ioanna D. Styliari, et al, ‘High‐Throughput Miniaturized Screening of Nanoparticle Formation via Inkjet Printing’, Macromolecular Materials and Engineering, (2018), which has been published in ...
Alexander, Cameron, Alexander, Morgan R., Burley, Jonathan, Cavanagh, Robert, Conte, Claudia, Couturaud, Benoit, Garnett, Martin, Gordhan, Dipak, Hook, Andrew, Huesler, Amanda, Limo, Marion, Nieto-Orellana, Alejandro, Pearce, Amanda, Styliari, Ioanna Danai, Suksiriworapong, Jiraphong, Taresco, Vincenzo, Williams, Phil   +16 more
core   +4 more sources