Results 91 to 100 of about 227,287 (224)

Composition and applications of focus libraries to phenotypic assays

Frontiers in Pharmacology, 2014
The wealth of bioactivity information now available on low-molecular weight compounds has enabled a paradigm shift in chemical biology and early phase drug discovery efforts.
Anne Mai eWassermann, Luiz Miguel Camargo, Douglas S. Auld   +2 more
doaj   +1 more source

Fully Automated OCT-based Tissue Screening System [PDF]

This study introduces a groundbreaking optical coherence tomography (OCT) imaging system dedicated for high-throughput screening applications using ex vivo tissue culture. Leveraging OCT's non-invasive, high-resolution capabilities, the system is equipped with a custom-designed motorized platform and tissue detection ability for automated, successive ...
arxiv   +1 more source

Development of a Ubiquitin Transfer Assay for High Throughput Screening by Fluorescence Resonance Energy Transfer [PDF]

, 2000
Michael D. Boisclair, Christopher McClure, Serene Josiah, Susan Glass, Steve Bottomley, Shubi M. Kamerkar, Ilkka Hemmilä   +6 more
openalex   +1 more source

NOVAsort for error-free droplet microfluidics

Nature Communications
High-throughput screening techniques are pivotal to unlocking the mysteries of biology. Yet, the promise of droplet microfluidics in enabling single-cell resolution, ultra-high-throughput screening remains largely unfulfilled.
Han Zhang, Rohit Gupte, Yuwen Li, Can Huang, Adrian R. Guzman, Jeong Jae Han, Haemin Jung, Rushant Sabnis, Paul de Figueiredo, Arum Han   +9 more
doaj   +1 more source

Protocol for high-throughput compound screening using flow cytometry in THP-1 cells

STAR Protocols, 2021
Summary: Flow cytometry is a valuable method for analyzing protein expressions at the single cell level but can be difficult to apply to large numbers of samples.
Stephan H. Spangenberg, Reza Beheshti Zavareh, Luke L. Lairson   +2 more
doaj  

Development of a Facile Method for High Throughput Screening with Reporter Gene Assays [PDF]

, 2000
Aaron S. Goetz, John L. Andrews, Thomas R. Littleton, Diane M. Ignar   +3 more
openalex   +1 more source

A kinetic intra-cellular assay (KICA) to measure quantitative compound binding kinetics within living cells

STAR Protocols, 2022
Summary: The Kinetic Intra-Cellular Assay (KICA) is a recombinant cell-based technique that utilizes NanoBRET technology. KICA enables the measurement of intracellular binding kinetics.
Charles S. Lay, Daniel A. Thomas, John P. Evans, Emma J. Jones, Kelly M. Gatfield, Peter D. Craggs   +5 more
doaj  

POLARISED views and FRETting about probe modulation assays: Learning from High Throughput Screening

SLAS Discovery
Fluorescent probe modulation assays are a widely used approach to monitor displacement or stabilisation of fluorescently labelled tool ligands by test compounds.
Alice Lanne, Catherine Bardelle, Gareth Davies, Antonia Turberville, Hannah Semple, Rachel Moore, Geoffrey A. Holdgate   +6 more
doaj  

High throughput screening for LC3/GABARAP binders utilizing fluorescence polarization assay [PDF]

arXiv
The characterization of interactions between autophagy modifiers (Atg8-family proteins) and their natural ligands (peptides and proteins) or small molecules is important for a detailed understanding of selective autophagy mechanisms and for design of potential Atg8 inhibitors that affect the autophagy processes in cells.
arxiv  

Machine Learning Harnesses Molecular Dynamics to Discover New $μ$ Opioid Chemotypes [PDF]

arXiv, 2018
Computational chemists typically assay drug candidates by virtually screening compounds against crystal structures of a protein despite the fact that some targets, like the $\mu$ Opioid Receptor and other members of the GPCR family, traverse many non-crystallographic states.
arxiv