Protocol for tissue slice cultures from human solid tumors to study therapeutic response
STAR Protocols, 2021 Summary: The impact of systemic therapy on the tumor microenvironment has been difficult to study in human solid tumors. Our protocol describes steps for establishing slice cultures to investigate response to chemotherapies, immunotherapies, or adoptive ...
Heidi L. Kenerson +4 more
doaj +1 more source
Analysis of high-throughput screening assays using cluster enrichment. [PDF]
Stat Med, 2012 In this paper, we describe the implementation and evaluation of a cluster‐based enrichment strategy to call hits from a high‐throughput screen using a typical cell‐based assay of 160,000 chemical compounds. Our focus is on statistical properties of the prospective design choices throughout the analysis, including how to choose the number of clusters ...
Pu M +7 more
europepmc +6 more sources
Frontiers in Microbiology, 2016 Nicotinamidases catalyze the hydrolysis of the amide bond in nicotinamide to produce ammonia and nicotinic acid. These enzymes are an essential component of the NAD+ salvage pathway and are implicated in the viability of several pathogenic organisms. Its
Rubén Zapata-Pérez +4 more
doaj +1 more source
Subtyping of Swine Influenza Viruses Using a High-Throughput Real-Time PCR Platform [PDF]
, 2018 Influenza A viruses (IAVs) are important human and animal pathogens with high impact on human and animal health. In Denmark, a passive surveillance program for IAV in pigs has been performed since 2011, where screening tests and subsequent subtyping are ...
Breum, Solvej Ø. +6 more
core +3 more sources
Assay in high throughput screening
Folia Pharmacologica Japonica, 2001 HTS (High Throughput Screening) has been put into practice in recent years. HTS is aiming to discover lead compounds for medicinal drugs. High efficiency must be achieved in all the processes including sample preparation, assay procedure, automation and data management.
openaire +3 more sources
FRET assay for live-cell high-throughput screening of the cardiac SERCA pump yields multiple classes of small-molecule allosteric modulators [PDF]
, 2023 Osha Roopnarine +8 more
openalex +3 more sources
Development and optimization of a miniaturized western blot-based screening platform to identify regulators of post-translational modifications [PDF]
, 2019 Post-translational modifications (PTMs) are fundamental traits of protein functionality and their study has been addressed using several approaches over the past years.
Gottifredi, Vanesa +2 more
core +1 more source
Advancing liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry toward ultra-high-throughput analysis [PDF]
, 2020 Label-free high-throughput screening using mass spectrometry has the potential to provide rapid large-scale sample analysis at a speed of more than one sample per second.
Brown, Jeffery +5 more
core +1 more source
Arbitrarily Accessible 3D Microfluidic Device for Combinatorial High-Throughput Drug Screening
Sensors, 2016 Microfluidics-based drug-screening systems have enabled efficient and high-throughput drug screening, but their routine uses in ordinary labs are limited due to the complexity involved in device fabrication and system setup.
Zhuofa Chen +6 more
doaj +1 more source
Quantify permeability using on-a-chip models in high-throughput applications
STAR Protocols, 2023 Summary: Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on the assumption of sink condition (concentration of the donor is constant, and the receiver increases less than 10%).
Camilla Soragni +7 more
doaj +1 more source