Results 41 to 50 of about 229,438 (321)

Methods to identify the unexplored diversity of microbial exopolysaccharides

Frontiers in Microbiology, 2015
Microbial exopolysaccharides (EPS) are a structurally very diverse class of molecules. A number of them have found their application in rather diverging fields that extend from medicine, food and cosmetics on the one side to construction, drilling and ...
Broder eRühmann, Jochen eSchmid, Volker eSieber   +2 more
doaj   +1 more source

Canvass: a crowd-sourced, natural-product screening library for exploring biological space [PDF]

, 2018
NCATS thanks Dingyin Tao for assistance with compound characterization. This research was supported by the Intramural Research Program of the National Center for Advancing Translational Sciences, National Institutes of Health (NIH). R.B.A.
Andrade, Rodrigo B., Bougie, Danielle, Boxer, Matthew B., Brimacombe, Kyle R., Brown, Lauren E., Brown, M Kevin, Burns, Noah Z., Cha, Jin K., Cheff, Dorian M., Cheng, Ken, Clardy, Jon, Clement, Jason A., Coussens, Nathan P., Crooks, Peter A., Cuny, Gregory D., Dillon, Christopher, Dorjsuren, Dorjbal, Eastman, Richard T., Ganor, Jake, Garg, Neil K., Goess, Brian C., Grossman, Robert B., Guha, Rajarshi, Hall, Matthew D., Henderson, Mark J., Huang, Ruili, Hughes, Chambers C., Iannotti, Michael J., Inglese, James, Itkin, Zina, Jadhav, Ajit, Johnston, Jeffrey N., Joullie, Madeleine M., Karavadhi, Surendra, Kearney, Sara E., Kinghorn, A. Douglas, Kingston, David G. I., Klumpp-Thomas, Carleen, Krische, Michael J., Kwon, Ohyun, Lee, Tobie D., Lynch, Caitlin, Maimone, Thomas J., Majumdar, Susruta, Maloney, Katherine N., Mevers, Emily E., Michael, Sam, Mohamed, Enas, Moreno, Jesus, Morrill, Lucas A., Murphy, Brian T., Nagorny, Pavel, Olson, David E., Overman, Larry E., Picazo, Elias, Porco, John A., Ren, Tianjing, Rivas, Fatima, Rohde, Jason M., Ross, Samir A., Roth, Jacob S., Sakamuru, Srilatha, Sarpong, Richmond, Sharma, Indrajeet, Shaw, Jared T., Shen, Ben, Shen, Min, Shi, Wei, Shinn, Paul, Simeonov, Anton, Snyder, John K., Stephenson, Corey R. J., Sun, Wei, Susick, Robert B., Tan, Derek S., Tang, Yi, Taylor, Richard E., Thomson, Regan J., Titus, Steven A., Verano, Alyssa L., Vosburg, David A., Wan, Kanny K., Wu, Jimmy, Wuest, William M., Xia, Menghang, Xu, Zhengren, Yasgar, Adam, Zahoranszky-Kohalmi, Gergely, Zakarian, Armen, Zhang, Ya-Qin, Zhang, Yufeng, Zhao, Jinghua, Zhao, Tongan, Zheng, Wei, Zuo, Zhong   +94 more
core   +1 more source

Protocol for tissue slice cultures from human solid tumors to study therapeutic response

STAR Protocols, 2021
Summary: The impact of systemic therapy on the tumor microenvironment has been difficult to study in human solid tumors. Our protocol describes steps for establishing slice cultures to investigate response to chemotherapies, immunotherapies, or adoptive ...
Heidi L. Kenerson, Kevin M. Sullivan, Kevin P. Labadie, Venu G. Pillarisetty, Raymond S. Yeung   +4 more
doaj   +1 more source

Combined whole-cell high-throughput functional screening for identification of new nicotinamidases/pyrazinamidases in metagenomic/polygenomic libraries

Frontiers in Microbiology, 2016
Nicotinamidases catalyze the hydrolysis of the amide bond in nicotinamide to produce ammonia and nicotinic acid. These enzymes are an essential component of the NAD+ salvage pathway and are implicated in the viability of several pathogenic organisms. Its
Rubén Zapata-Pérez, Antonio Ginés García-Saura, Mohamed Jebbar, Peter Golyshin, Álvaro Sánchez-Ferrer   +4 more
doaj   +1 more source

Assay in high throughput screening

Folia Pharmacologica Japonica, 2001
HTS (High Throughput Screening) has been put into practice in recent years. HTS is aiming to discover lead compounds for medicinal drugs. High efficiency must be achieved in all the processes including sample preparation, assay procedure, automation and data management.
openaire   +3 more sources

Arbitrarily Accessible 3D Microfluidic Device for Combinatorial High-Throughput Drug Screening

Sensors, 2016
Microfluidics-based drug-screening systems have enabled efficient and high-throughput drug screening, but their routine uses in ordinary labs are limited due to the complexity involved in device fabrication and system setup.
Zhuofa Chen, Weizhi Li, Gihoon Choi, Xiaonan Yang, Jun Miao, Liwang Cui, Weihua Guan   +6 more
doaj   +1 more source

A system for performing high throughput assays of synaptic function. [PDF]

PLoS ONE, 2011
Unbiased, high-throughput screening has proven invaluable for dissecting complex biological processes. Application of this general approach to synaptic function would have a major impact on neuroscience research and drug discovery.
Chris M Hempel, Michael Sivula, Jonathan M Levenson, David M Rose, Bing Li, Ana C Sirianni, Eva Xia, Timothy A Ryan, David J Gerber, Jeffrey R Cottrell   +9 more
doaj   +1 more source

Development and optimization of a miniaturized western blot-based screening platform to identify regulators of post-translational modifications [PDF]

, 2019
Post-translational modifications (PTMs) are fundamental traits of protein functionality and their study has been addressed using several approaches over the past years.
Gottifredi, Vanesa, Soria, Ramiro Gaston, Villafañez, Florencia   +2 more
core   +1 more source

Docking-Based Virtual Screening Enables Prioritizing Protein Kinase Inhibitors With In Vitro Phenotypic Activity Against Schistosoma mansoni

Frontiers in Cellular and Infection Microbiology, 2022
Schistosomiasis is a parasitic neglected disease with praziquantel (PZQ) utilized as the main drug for treatment, despite its low effectiveness against early stages of the worm. To aid in the search for new drugs to tackle schistosomiasis, computer-aided
Bernardo Pereira Moreira, Izabella Cristina Andrade Batista, Naiara Clemente Tavares, Tom Armstrong, Sandra Grossi Gava, Gabriella Parreiras Torres, Marina Moraes Mourão, Franco H. Falcone   +7 more
doaj   +1 more source

Optimization of Spectrophotometric and Fluorometric Assays Using Alternative Substrates for the High-Throughput Screening of Lipase Activity

Journal of Chemistry, 2021
The effects of reaction conditions on the spectrophotometric and fluorometric assays using alternative substrates (p-nitrophenyl palmitate and 4-methylumbelliferyl oleate) were investigated to optimize them for the high-throughput screening of lipase ...
Jun-Young Park, Jisu Ha, Yoonseok Choi, Pahn-Shick Chang, Kyung-Min Park   +4 more
doaj   +1 more source