Results 41 to 50 of about 227,287 (224)
Communications Biology, 2022 High-throughput screening of 120,000 compounds followed by counter-screening and validation assays reveals candidate antimalarial drugs that kill Plasmodium falciparum sexual and asexual blood stages blocking parasite transmission through mosquito.
Giacomo Paonessa +13 more
doaj +1 more source
Estimation of In-Vitro Free Radical Scavenging and Cytotoxic Activities of Thiocarbohydrazones Derivatives [PDF]
arXiv, 2023 Objective: To investigate free radical scavenging potential of thiocarbohydrazones derivatives for the discovery of antioxidant compounds of synthetic origin. Method: Eighteen thiocarbohydrazones derivatives were screened for antioxidant activities by using in vitro radical inhibition assay.
arxiv
Journal of biomolecular screening, 2007 The z-score method and its variants for testing mean difference are commonly used for hit selection in high-throughput screening (HTS) assays. Strictly standardized mean difference (SSMD) offers a way to measure and classify the short interfering RNA ...
Xiaohua Douglas Zhang
semanticscholar +1 more source
High-throughput screening assay used in pharmacognosy: Selection, optimization and validation of methods of enzymatic inhibition by UV-visible spectrophotometry [PDF]
Journal of Pharmacy & Pharmacognosy Research, 2014 In research laboratories of both organic synthesis and extraction of natural products, every day a lot of products that can potentially introduce some biological activity are obtained.
Graciela Granados-Guzmán +3 more
doaj
Journal of biomolecular screening, 2014 Estrogen receptor (ER) is a ligand-inducible transcriptional factor involving in cell growth, differentiation, and diseases, so detection and identification of compounds having estrogenic effects are of great importance in the drug discovery industry. We
Caihua Wang +3 more
semanticscholar +1 more source
STAR Protocols, 2021 Summary: Autophagy measurement has been challenging due to the transient nature of autophagy vesicles, in which degradation of cargo occurs. Here, we present a protocol to monitor starvation-induced autophagy using a live high-throughput microscopy ...
Ada Nowosad, Arnaud Besson
doaj
GSSMD: New metric for robust and interpretable assay quality assessment and hit selection [PDF]
arXiv, 2020 In the high-throughput screening (HTS) campaigns, the Z'-factor and strictly standardized mean difference (SSMD) are commonly used to assess the quality of assays and to select hits. However, these measures are vulnerable to outliers and their performances are highly sensitive to background distributions.
arxiv
A Public Website for the Automated Assessment and Validation of SARS-CoV-2 Diagnostic PCR Assays [PDF]
arXiv, 2020 Summary: Polymerase chain reaction-based assays are the current gold standard for detecting and diagnosing SARS-CoV-2. However, as SARS-CoV-2 mutates, we need to constantly assess whether existing PCR-based assays will continue to detect all known viral strains.
arxiv
STAR Protocols, 2023 Summary: The complement receptors C3aR and C5aR1 are promising therapeutic targets. Here, we present a protocol to screen the effects of different agonists and antagonists on these receptors in vitro, using phosphorylated extracellular signal-regulated ...
Xaria X. Li, Trent M. Woodruff
doaj
Validation of a modified fluorimetric assay for the screening of trichomonacidal drugs
Memorias do Instituto Oswaldo Cruz, 2012 A fluorimetric microassay that uses a redox dye to determine the viability of the flagellate Trichomonas vaginalis has been optimised to provide a more sensitive method to evaluate potential trichomonacidal compounds.
Alexandra Ibáñez Escribano +6 more
doaj +1 more source