Comparison of 2-Aminobenzamide, Procainamide and RapiFluor-MS as Derivatizing Agents for High-Throughput HILIC-UPLC-FLR-MS N-glycan Analysis [PDF]
Frontiers in Chemistry, 2018 Rising awareness of the universal importance of protein N-glycosylation governs the development of further advances in N-glycan analysis. Nowadays it is well known that correct glycosylation is essential for proper protein function, which emanates from ...
Toma Keser +2 more
exaly +8 more sources
Pregnancy-related changes in the canine serum N-glycosylation pattern studied by Rapifluor HILIC-UPLC-FLR-MS [PDF]
Scientific ReportsCanine reproduction differs from that of many other domestic animals, and increased knowledge on biochemical changes during canine pregnancy is important for investigations of infertility or subfertility.
Margareta Ramström +3 more
doaj +6 more sources
A Benchtop Approach To Measuring S-AdenosylMethionine Metabolite Levels Using HILIC UPLC-MS [PDF]
Journal of the Kentucky Academy of Science, 2019 S-adenosylmethionine (SAMe) is the most common methyl donor found in living organisms. It is the second most common cellular enzyme substrate and is an essential molecule in the carbon metabolic cycle. SAMe is widely used in both a clinical setting and as a nutritional supplement to treat a variety of disorders ranging from liver disease to ...
Erin D Strome
exaly +4 more sources
A Novel Ultra‐Performance Liquid Chromatography‐Electrospray Ionisation‐Tandem Mass Spectrometry Method for Quantification of Imeglimin in Human Plasma: Application to a Bioequivalence Study [PDF]
Analytical Science AdvancesThe pharmaceutical market has recently witnessed the advent of a novel tetrahydrotriazene molecule belonging to the new pharmacological class “Glimins”, named Imeglimin (IMG).
Mina Wadie +5 more
doaj +2 more sources
Establishment of an N-Glycan Profiling Method for Three ERT Enzymes Used in Gaucher Disease Therapy [PDF]
MoleculesN-glycosylation, particularly terminal mannose exposure, is a critical quality attribute affecting macrophage targeting and the clinical efficacy of enzyme replacement therapy for Gaucher disease.
Jinliang Chen +6 more
doaj +2 more sources
Determination of Free Amino Acids in Isatidis Radix By HILIC-UPLC-MS/MS
Bulletin of the Korean Chemical Society, 2014 ranges. The limits of detection (LOD) and limits of quantitation (LOQ) for these compounds were 0.21-79.55 and 0.72-294.23 ng/mL, respectively. The average recoveries were in the range of 93.75-104.16% with RSD value less than 6.56%. Therefore, this method could be an alternative assay for the determination of 22 amino acids in Isatidis Radix due to ...
Jianwei Chen
exaly +3 more sources
Differences in canine serum N-glycosylation pattern during infection and pregnancy [PDF]
Scientific ReportsDiagnosis of inflammatory disorders during canine pregnancy is complicated by changes caused by pregnancy. The total glycosylation pattern of body fluids reflects cellular status in health and disease.
Margareta Ramström +4 more
doaj +2 more sources
NMR联用HILIC-UPLC-MS/MS测定TNBS诱导的结肠炎大鼠尿液和血浆代谢物变化
Zhongshan Daxue xuebao. Yixue kexue ban, 2021 目的基于非靶标和靶标代谢组学技术，探讨三硝基苯磺酸（TNBS）诱导的结肠炎模型大鼠尿液以及血浆中内源性代谢物的变化。方法16只雄性SD大鼠随机分成对照组和模型组，每组8只，对模型组大鼠给予TNBS灌肠以建立实验性大鼠结肠炎模型。利用核磁共振氢谱（1H NMR）检测两组大鼠尿液代谢物，同时联用HILIC模式超高效液相色谱串联三重四级杆质谱（HILIC-UPLC-MS/MS）测定以上大鼠尿液以及血浆中氨基酸水平，并通过多元变量统计方法对代谢组学数据进行分析。结果同对照组相比，1H ...
刘雯 +7 more
doaj +1 more source
Immunoglobulin G N-glycosylation predicts long-term risk of ischemic stroke: a nested case-control study [PDF]
Journal of Translational MedicineBackground Ischemic stroke (IS) ranks as the leading cause of disability and the second cause of mortality in adults worldwide. This investigation aimed to describe the fingerprint of immunoglobulin G (IgG) N-glycans and feature of circulating ...
Shuyu Sun +14 more
doaj +2 more sources
International Journal of Analytical Chemistry, 2022 A new method was developed for the identification and determination of L-ergothioneine in cosmetics based on ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
Li Liu +8 more
doaj +1 more source