Results 101 to 110 of about 435,238 (305)

Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]

, 1993
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Amman, Atkinson, Baekkeskov, Baekkeskov, Berthold, Boitard, Bottazzo, Castano, Dean, Frorath, Goedel, Greenbaum, Harrison, Heike Berg, Hopp, Jochen Seissler, Karjalainen, Kaufman, Kuglin, Kuglin, Ludwig Mauch, Michael Walter, Palmer, Patrick, Seissler, Smith, Smith, Snyder, Su, Wolfgang Northemann   +29 more
core   +1 more source

Thiolated Polymers in 3D Bioprinting: Control of Gelation

Advanced Materials, EarlyView.
Thiolated polymers are established as programmable bioinks for 3D bioprinting, integrating versatile crosslinking chemistries with redox‐responsive control. This work demonstrates how molecular design and external triggers define gelation kinetics, printability windows, and structural fidelity, enabling stable, high‐resolution constructs and advancing ...
Soheil Haddadzadegan, Flavia Laffleur, Andreas Bernkop‐Schnürch   +2 more
wiley   +1 more source

Exploring the Potential of Zero‐Dimensional Carbon Nanomaterials in Photoluminescent, Electrochemiluminescent and Electrochemical Sensors

Advanced Materials Interfaces, EarlyView.
Zero‐dimensional carbon nanomaterials are presented as multifunctional platforms linking structure, property, and sensing performance. Surface engineering and heteroatom doping modulate electron‐transfer and luminescent behavior, enabling electrochemical, photoluminescent, and electrochemiluminescent detection. Fundamental design principles, analytical
Gustavo Martins, Jeferson Luiz Gogola, Ava Gevaerd, Cristiane Kalinke, Paulo Roberto de Oliveira, Fabio Roberto Caetano, Bruno Campos Janegitz, Márcio Fernando Bergamini, Luiz Humberto Marcolino‐Junior   +8 more
wiley   +1 more source

Review: The roles and functions of histidine on growth performance and intestinal health of weaning piglets

Animal Nutriomics
Histidine is an essential limiting amino acid in piglet diets and plays a crucial role in pig production. Supplementing piglet diets with adequate histidine can enhance growth performance, promote protein synthesis, regulate lipid metabolism, and improve
Hanxiao Yang, Zongyong Jiang, Shuting Cao, Li Wang   +3 more
doaj   +1 more source

Impact of decitabine on immunohistochemistry expression of the putative tumor suppressor genes FHIT, WWOX, FUS1 and PTEN in clinical tumor samples. [PDF]

, 2014
BackgroundSince tumor suppressor gene function may be lost through hypermethylation, we assessed whether the demethylating agent decitabine could increase tumor suppressor gene expression clinically.
Aldaz, Marcelo, Gupta, Sanjay, Hong, David, Issa, Jean-Pierre, Jelinek, Jaroslav, Kurzrock, Razelle, Nunez, Maria I, Stewart, David J, Wistuba, Ignacio I   +8 more
core   +3 more sources

QBP1 Peptide as a Potential Anti‐Amyloidogenic Therapy for Type 2 Diabetes: An In Vitro Study

Advanced Science, EarlyView.
The anti‐amyloidogenic peptide QBP1 effectively halts human islet amyloid polypeptide (hIAPP) aggregation, preventing the formation of toxic β‐structured intermediates. Through a combination of biophysical assays, molecular dynamics, and cell‐based studies, QBP1 is shown to preserve β‐cell viability and metabolic homeostasis, positioning it as a ...
María M. Tejero‐Ojeda, Ada Bernaus Vives, Michał Wojciechowski, Dinh Quoc Huy Pham, Mateusz Chwastyk, Mario Vallejo, Anna Novials, Douglas V. Laurents, Mariano Carrión‐Vázquez   +8 more
wiley   +1 more source

Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity

Advanced Science, EarlyView.
This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley   +1 more source

Deuterium trafficking, mitochondrial dysfunction, copper homeostasis, and neurodegenerative disease

Frontiers in Molecular Biosciences
Deuterium is a natural heavy isotope of hydrogen, containing an extra neutron. Eukaryotic organisms have devised complex metabolic policies that restrict the amount of deuterium reaching the mitochondria, because it damages the ATPase pumps, leading to ...
Stephanie Seneff, Anthony M. Kyriakopoulos, Anthony M. Kyriakopoulos   +2 more
doaj   +1 more source

Block of Kcnk3 by protons. Evidence that 2-P-domain potassium channel subunits function as homodimers. [PDF]

, 2001
KCNK subunits have two pore-forming P domains and four predicted transmembrane segments. To assess the number of subunits in each pore, we studied external proton block of Kcnk3, a subunit prominent in rodent heart and brain.
Goldstein, SA, Lopes, CM, Zilberberg, N
core  

Functional Analysis of Ligand‐Gated Chloride Channels in a Cnidarian Sheds Light on the Evolution of Inhibitory Signaling

Advanced Science, EarlyView.
We uncover a large variety of putative inhibitory ligand‐gated ion channels (LGICs) in the phylum Cnidaria, the sister group to all bilaterian animals. Phylogenetic analysis suggests a complex evolutionary history of inhibitory LGICs with diverse neurotransmitter ligands.
Abhilasha Ojha, Linda Kloss, Juan D. Montenegro, Simone Albani, Audrey Ortega‐Ramírez, Mihaela Raycheva, Sylvia Joussen, Sabrina Kaul, Michèle Bachmann, Lisa Huf, Günther Schmalzing, Alison G. Cole, Ulrich Technau, Stefan Gründer   +13 more
wiley   +1 more source