Isolation, identification, and genomic analysis of bacteriophage GJKY-A for the prevention and treatment of citrus canker. [PDF]
Front MicrobiolWu Y +8 more
europepmc +1 more source
An efficient CRISPR-Cas12a tool for iterative genome editing, streamlined minimization, and payload engineering of <i>Pseudomonas</i> phage S1. [PDF]
Synth Syst BiotechnolLiu Y +5 more
europepmc +1 more source
Editorial: Phage-based interventions in livestock: from genomics to translational applications. [PDF]
Front MicrobiolZapata-Mena S +2 more
europepmc +1 more source
Decoding the molecular properties of mycobacteriophage D29 Holin provides insights into Holin engineering. [PDF]
Journal of Virology, 2021 Holins are bacteriophage-encoded small membrane perforators that play an important role in determining the timing of host cell lysis toward the end of the phage infection cycle. The ability of holin to precisely time the hole formation in the cell membrane, ensuring cell lysis, is both interesting and intriguing.
Vikas Jain, Varun Rakeshbhai Bavda
exaly +3 more sources
Related searches:
Holin-assisted bacterial recombinant protein export
Biotechnology and Bioengineering, 2022AbstractA simple generic method for enhancing extracellular protein yields in engineered bacteria is still lacking. Here, we demonstrated that phage‐encoded holin can be used to export proteins to the extracellular medium in both Gram‐negative Escherichia coli and ‐positive Lactococcus lactis.
Jian Kong
exaly +3 more sources
Functional analysis of the phage T4 holin in a λ context
Molecular Genetics and Genomics, 2001Phage lambda hybrids were constructed by inserting the t gene of phage T4 in place of the lambda holin gene, S. Induction of the hybrid phage resulted in lysis that was just as abrupt as, but occurred much earlier in the vegetative cycle than, that obtained with lambda, indicating that t is indeed a holin gene. Moreover, it was possible to impose lysis
Yerlan Ramankulov, Ry Young
exaly +3 more sources
Holins: The Protein Clocks of Bacteriophage Infections
Annual Review of Microbiology, 2000▪ Abstract Two proteins, an endolysin and a holin, are essential for host lysis by bacteriophage. Endolysin is the term for muralytic enzymes that degrade the cell wall; endolysins accumulate in the cytosol fully folded during the vegetative cycle. Holins are small membrane proteins that accumulate in the membrane until, at a specific time that is ...
I N, Wang, D L, Smith, R, Young
openaire +2 more sources
Probing the function of the two holin-like proteins of bacteriophage SPP1
Virology, 2017 Double-stranded DNA bacteriophages employ holin and endolysin functions to lyse host bacteria after virus multiplication. Holins oligomerize in the cytoplasmic membrane and trigger to form holes that cause cell death. For most systems these holes are also required for endolysin release to the cell wall, where it cleaves the peptidoglycan network.
Carlos São-José, Sofia Fernandes
exaly +3 more sources
Holins: form and function in bacteriophage lysis
FEMS Microbiology Reviews, 1995During the lytic cycle of most bacteriophages, a phage-encoded peptidoglycan-degrading activity is elaborated. At least four entirely distinct types of enzymes fulfill this role and are given the generic name 'endolysin'. Endolysins characterized to date are synthesized without a signal sequence and thus accumulate fully folded and active in the ...
R, Young, U, Bläsi
openaire +3 more sources
Genetic analysis of the T4 holin: timing and topology
Gene, 2001The t protein of bacteriophage T4 shares with other holins the ability to cause the formation of a lethal membrane lesion which allows the phage endolysin to attack the peptidoglycan. Moreover, T, like other holins, acts in a saltatory manner at a precisely programmed time in the vegetative cycle.
E, Ramanculov, R, Young
openaire +2 more sources