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Topological examination of the bacteriophage lambda S holin by EPR spectroscopy
Biophysical Journal, 2022The S protein from bacteriophage lambda is a three-helix transmembrane protein produced by the prophage which accumulates in the host membrane during late gene expression. It is responsible for the first step in lysing the host cell at the end of the viral life cycle by multimerizing together to form large pores which permeabilize the host membrane to ...
Andrew K, Morris +3 more
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A coupling process for improving purity of bacterial minicells by holin/lysin
Journal of Microbiological Methods, 2011Drug and gene delivery systems using bacterial minicells have attracted much attention. Here we attempted to enhance the yield and purity of the minicells using a novel method in which autolysin induced in actively metabolizing parent cells led to autolysis. A two-step protocol coupling an autolysin (holin/lysin) step with a conventional centrifugation
Hyon E Choy, Jin-Young Lee
exaly +3 more sources
Molecular function of the dual‐start motif in the λ S holin
Molecular Microbiology, 1999The λS gene represents the prototype of holin genes with a dual‐start motif, which leads to the synthesis of two polypeptides, S105 and S107. They differ at their N‐terminus by only two amino acids, Met‐1 and Lys‐2, at the beginning of the longer product. Despite the minor difference, the two proteins have opposing functions in lysis, with protein S107
A, Graschopf, U, Bläsi
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Cloning and sequencing of a new holin-encoding gene of Bacillus licheniformis
Gene, 1996A Bacillus licheniformis DNA fragment which exhibits homology with the upstream region of the cell-wall hydrolase-encoding gene, cwlL, was cloned into Escherichia coli (Ec). Nucleotide sequencing indicated that there are two open reading frames (tentatively designated as xpaG1 and xpaG2) which encode polypeptides of 89 and 88 amino acids (aa) (10044 ...
K, Kyogoku, J, Sekiguchi
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The Holin-Endolysin Lysis System of the OP2-Like Phage X2 Infecting Xanthomonas oryzae pv. oryzae
Viruses, 2021Bin Li, Sebastian Leptihn, Belinda Loh
exaly
Bactericidal activity of a holin-endolysin system derived from phage HH109
Microbial Pathogenesis, 2021Zhe Zhao, Fei Yu, Ce Zhang
exaly
Prediction and identification of new type holin protein of Escherichia coli phage ECP26
Food Science and Biotechnology, 2022Jong-Hyun Park +2 more
exaly
A holin/peptidoglycan hydrolase-dependent protein secretion system
Molecular Microbiology, 2021Tracy Palmer +2 more
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