Results 1 to 10 of about 135 (79)
Phage Spanins: Diversity, Topological Dynamics and Gene Convergence [PDF]
BMC Bioinformatics, 2018 Background Spanins are phage lysis proteins required to disrupt the outer membrane. Phages employ either two-component spanins or unimolecular spanins in this final step of Gram-negative host lysis.
Manoj Rajaure, Ry Young, Jesse L Cahill
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Genetic Analysis of the Lambda Spanins Rz and Rz1: Identification of Functional Domains
G3: Genes, Genomes, Genetics, 2017 Coliphage lambda proteins Rz and Rz1 are the inner membrane and outer membrane subunits of the spanin complex—a heterotetramer that bridges the periplasm and is essential for the disruption of the outer membrane during phage lysis.
Manoj Rajaure, Ry Young, Jesse L Cahill
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Harnessing a T1 Phage-Derived Spanin for Developing Phage-Based Antimicrobial Development
Biodesign ResearchThe global increase in the prevalence of drug-resistant bacteria has necessitated the development of alternative treatments that do not rely on conventional antimicrobial agents.
Aa Haeruman Azam +2 more
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Spanin Function Requires Subunit Homodimerization Through Intermolecular Disulfide Bonds [PDF]
Molecular Microbiology, 2013 SummaryThe λ Rz and Rz1 proteins are the subunits of the spanin complex, required for the disruption of the outer membrane during host lysis. Rz, the inner membrane or i‐spanin, has a largely alpha‐helical periplasmic domain, whereas Rz1, the outer membrane or o‐spanin, has a 25% proline content with no predicted secondary structure.
Manoj Rajaure, Ry Young
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The Spanin Complex Is Essential for Lambda Lysis. [PDF]
Journal of Bacteriology, 2012 ABSTRACT Phage lysis is a ubiquitous biological process, the most frequent cytocidal event in the biosphere. Lysis of Gram-negative hosts has been shown to require holins and endolysins, which attack the cytoplasmic membrane and peptidoglycan, respectively. Recently, a third class of lysis proteins, the spanins, was identified.
Ting Pang, Manoj Rajaure, Ry Young
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Localization and Regulation of the T1 Unimolecular Spanin. [PDF]
Journal of Virology, 2018 Spanins have been proposed to fuse the cytoplasmic and outer membranes during phage lysis. Recent work with the lambda spanins Rz-Rz1, which are similar to class I viral fusion proteins, has shed light on the functional domains and requirements for two-component spanin function.
Ry Young, Rohit Kongari
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The Lambda Spanin Components Rz and Rz1 Undergo Tertiary and Quaternary Rearrangements Upon Complex Formation [PDF]
Protein Science, 2010 AbstractPhage holins and endolysins have long been known to play key roles in lysis of the host cell, disrupting the cytoplasmic membrane and peptidoglycan (PG) layer, respectively. For phages of Gram‐negative hosts, a third class of proteins, the spanins, are involved in disrupting the outer membrane (OM).
Ry Young, Andreas Holzenburg
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Structural Insights Into Membrane Fusion Mediated by Convergent Small Fusogens
Cells, 2021 From lifeless viral particles to complex multicellular organisms, membrane fusion is inarguably the important fundamental biological phenomena. Sitting at the heart of membrane fusion are protein mediators known as fusogens.
Yiming Yang, Nandini Nagarajan Margam
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Enzymes and Mechanisms Employed by Tailed Bacteriophages to Breach the Bacterial Cell Barriers
Viruses, 2018 Monoderm bacteria possess a cell envelope made of a cytoplasmic membrane and a cell wall, whereas diderm bacteria have and extra lipid layer, the outer membrane, covering the cell wall.
Carlos São-José, Sofia Fernandes
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The Lysis Cassette of Jumbophage PhiKZ
Scientific ReportsPseudomonas jumbophage phiKZ is prominent because of the proteinaceous nuclear structure that is formed during the infection cycle, protecting the replicating phage DNA from host nucleases.
Ry Young +2 more
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