Human adipose-derived stem cells localize in the stromal-vascular portion, and can be ex vivo isolated using a combination of washing steps and enzymatic digestion.
Pietro Gentile +2 more
doaj +6 more sources
Development of a Biodegradable Microcarrier for the Cultivation of Human Adipose Stem Cells (hASCs) with a Defined Xeno- and Serum-Free Medium [PDF]
Stirred single-use bioreactors in combination with microcarriers (MCs) have established themselves as a technology that has the potential to meet the demands of current and future cell therapeutic markets. However, most of the published processes have been performed using fetal bovine serum (FBS) containing cell culture medium and non-biocompatible MCs.
Francesco Muoio +8 more
openaire +4 more sources
The osteogenic differentiation of SSEA-4 sub-population of human adipose derived stem cells using silicate nanoplatelets [PDF]
How to surpass invitro stem cell differentiation, reducing cell manipulation, and lead the in situ regeneration process after transplantation, remains to be unraveled in bone tissue engineering (bTE).
Gaharwar, Akhilesh K. +5 more
core +3 more sources
Epithelial differentiation of human adipose-derived stem cells (hASCs) undergoing three-dimensional (3D) cultivation with collagen sponge scaffold (CSS) via an indirect co-culture strategy [PDF]
Background Three-dimensional (3D) cultivation with biomaterials was proposed to facilitate stem cell epithelial differentiation for wound healing.
Minxiong Li +8 more
doaj +3 more sources
Osteogenic potential of human adipose derived stem cells (hASCs) seeded on titanium trabecular spinal cages [PDF]
AbstractSpine degenerative conditions are becoming increasingly prevalent, affecting about 5.7% of the population in Europe, resulting in a significant reduction of life’s quality. Up to now, many materials have been used in manufacturing cage implants, used as graft substitutes, to achieve immediate and long-term spinal fixation.
Laura Caliogna +9 more
openaire +3 more sources
Rapid Generation of Fusable Cell Beads for Multi-Scale Human Living Materials Assembly. [PDF]
Living beads are produced through the combination of metabolic glycoengineering, click chemistry, and superhydrophobic surfaces, to develop a spheroid alternative for bottom‐up tissue engineering and disease modeling. The living beads present a size independent of cell density and a rapid fabrication methodology, providing a platform that can fuse into
Moura BS +5 more
europepmc +2 more sources
Adipose-Derived Stem Cells as Therapeutic Approach in Hypertrophic Scar Formation-A Systematic Review. [PDF]
ABSTRACT Despite numerous therapeutic approaches, the inhibition of hypertrophic scar formation remains a major challenge. Adipose‐derived stem cells (ADSCs) have been shown to improve wound healing, including remodelling, in vivo. A systematic review was conducted using the electronic databases PubMed, Web of Science, Embase and Medline.
Kohlhauser M +5 more
europepmc +2 more sources
Human amnion-derived mesenchymal stem cells promote osteogenic and angiogenic differentiation of human adipose-derived stem cells. [PDF]
Tissue engineering using suitable mesenchymal stem cells (MSCs) shows great potential to regenerate bone defects. Our previous studies have indicated that human amnion-derived mesenchymal stem cells (HAMSCs) could promote the osteogenic differentiation ...
Chunli Zhang +3 more
doaj +1 more source
In Vivo Evaluation of Injected and Bioprinted Hyaluronic Acid-Based Bioink in Corneal Stromal Pocket. [PDF]
Hyaluronic acid‐based bioinks support the viability of cells and keratocyte‐like characteristics. They integrate with host cornea stroma and adapt to irregular pockets through stable in situ gelation. The bioinks remain optically transparent, promote tissue remodeling, and reduce haze and inflammation.
Kethiri AR +7 more
europepmc +2 more sources
Generation of human induced pluripotent stem cells from adipose-derived stromal/stem cells isolated from a 75-year-old patient [PDF]
Human adipose-derived stromal/stem cells (hASCs) have been considered a valuable cell source for generating induced pluripotent stem cells (iPSCs). Adipose tissue is easy to obtain.
Stoyanova Elena +2 more
doaj +1 more source

