Results 81 to 90 of about 113,516 (292)

Phenotyping cultured human cardiomyocytes.

, 2014
Human cardiomyocytes cultured on microscope cover slips where phenotyped using cardiac markers (A) α-MHC (63× magnification) (AF546-labeled secondary antibody [red]) (B) Cx43 (x40) (AF488-labeled primary antibody [green]) and (C) cTnT (40× magnification)
Lynn Morris (7883), Walter R. Lopes de Campos (646288), Lia S. Rotherham (129222), Makobetsa Khati (129233), Grace London (646290), Bongani M. Mayosi (202901), Nthato Chirwa (646289)   +6 more
core   +1 more source

Mechanical and Electrical Phenotype of hiPSC‐Cardiomyocytes on Fibronectin‐Based Hydrogels

Advanced Healthcare Materials, EarlyView.
We introduce fibronectin‐based PEG hydrogels with controlled rigidity to enable the culture of iPSC‐derived cardiomyocytes. These substrates offer an alternative to the current culture of these cells on fibronectin‐coated glass, providing enhanced structural and functional behavior. The system provides a more physiologically relevant platform to assess
Ana Da Silva Costa, Lineta Stonkute, Sara Trujillo, Mariana Azevedo Gonzalez Oliva, Francis Burton, Matthew J. Dalby, Oana Dobre, Godfrey Smith, Manuel Salmeron‐Sanchez   +8 more
wiley   +1 more source

In silico predictions of drug-induced changes in human cardiac contractility align with experimental recordings

Frontiers in Pharmacology
Drug-induced changes in cardiac contractility (inotropy) can lead to cardiotoxicity, a major cause of discontinuation in drug development. Preclinical approaches to assess cardiac inotropy are imperfect, with in vitro assays limited to stem cell-derived ...
Cristian Trovato, Cristian Trovato, Stefano Longobardi, Elisa Passini, Kylie A. Beattie, Maxx Holmes, Khuram W. Chaudhary, Eric I. Rossman, Blanca Rodriguez   +8 more
doaj   +1 more source

Infusible Extracellular Matrix Biomaterial Enhances Cell‐Specific Pro‐Repair Responses Following Acute Myocardial Infarction

Advanced Healthcare Materials, EarlyView.
We measure the cell‐specific responses of administering infusible ECM (iECM) in acute myocardial infarction (MI) across multiple timepoints. Using single‐nucleus RNA sequencing and spatial transcriptomics, we measure macrophage activation, fibroblast remodeling, increased vascular development, lymphangiogenesis, cardioprotection, and neurogenesis ...
Joshua M. Mesfin, Alexander Chen, Quincy P. Lyons, Michael B. Nguyen, Maria L. Karkanitsa, Justin Yu, Van K. Ninh, Emily Gardner, Elyse G. Wong, Sriya N. Paleti, Julian Cheng, Benjamin D. Bridgelal, Kate E. Reimold, Selena Cao, Connor Uhre, Colin G. Luo, Zhenxing Fu, Kevin R. King, Karen L. Christman   +18 more
wiley   +1 more source

Antiarrhythmic effects of dantrolene in human diseased cardiomyocytes

Heart Rhythm, 2017
Cardiac type 2 ryanodine receptors (RyR2s) play a pivotal role in cellular electrophysiology and contractility. Increased RyR2-mediated diastolic sarcoplasmic reticulum (SR) Ca2+ release is linked to heart failure (HF) and arrhythmias. Dantrolene, a drug used for the treatment of malignant hyperthermia, is known to stabilize RyRs in skeletal muscle.The
Hartmann, Nico H., Pabel, Steffen, Herting, Jonas, Schatter, Felix, Renner, André, Gummert, Jan, Schotola, Hanna, Danner, Bernhard C., Maier, Lars S., Frey, Norbert, Hasenfuß, Gerd, Fischer, Andre, Sossalla, Samuel   +12 more
openaire   +3 more sources

Chamber‐Specific Decellularized Extracellular Matrices Differentially Modulate Cardiomyocyte Subtypes to Drive Engineered Heart Tissue Development and Function

Advanced Healthcare Materials, EarlyView.
Chamber‐specific decellularized extracellular matrices (ECMs) were developed, preserving native proteomic profiles of ventricular and atrial myocardium. These innate biochemical cues differentially modulate cardiomyocyte subtypes to drive engineered heart tissue development and function, highlighting the importance of incorporating regional ECM cues in
Dong Gyu Hwang, Myungji Kim, Hwanyong Choi, Jinseon Park, Minji Kim, Donghwan Kim, Bo Ram Lee, Jinah Jang   +7 more
wiley   +1 more source

Generation and characterization of functional cardiomyocytes derived from human T cell-derived induced pluripotent stem cells. [PDF]

PLoS ONE, 2014
Induced pluripotent stem cells (iPSCs) have been proposed as novel cell sources for genetic disease models and revolutionary clinical therapies. Accordingly, human iPSC-derived cardiomyocytes are potential cell sources for cardiomyocyte transplantation ...
Tomohisa Seki, Shinsuke Yuasa, Dai Kusumoto, Akira Kunitomi, Yuki Saito, Shugo Tohyama, Kojiro Yae, Yoshikazu Kishino, Marina Okada, Hisayuki Hashimoto, Makoto Takei, Toru Egashira, Masaki Kodaira, Yusuke Kuroda, Atsushi Tanaka, Shinichiro Okata, Tomoyuki Suzuki, Mitsushige Murata, Jun Fujita, Keiichi Fukuda   +19 more
doaj   +1 more source

Telomere‐independent cellular senescence in human fetal cardiomyocytes [PDF]

Aging Cell, 2004
SummaryFetal cardiomyocytes have been proposed as a potential source of cell‐based therapy for heart failure. This study examined cellular senescence in cultured human fetal ventricular cardiomyocytes (HFCs). HFCs were isolated and identified by immunocytochemistry and RT‐PCR.
Ball, Andrew J, Levine, F
openaire   +3 more sources

Coaxially Electrospun Myocardial dECM‐ Based Nanofibrous Scaffolds Demonstrate Enhanced Cardiomyocyte Biocompatibility

Advanced Healthcare Materials, EarlyView.
Our coaxial electrospinning system is capable of incorporating decellularized myocardial extracellular matrix in a core‐sheath configuration, creating nanofibrous scaffolds. These scaffolds, in turn, support the maintenance of functional cardiomyocytes adhered to the surface of these bioactive scaffolds.
Dhanusha N. Rajapakse, Mahtab Khodadadi, Kiran M. Ali, Suh Hee Cook, Bryson T. Proctor, Tanya Upadhyay, Daxian Zha, Fatema Tuj Jahura, Marziyeh Boosaliki, Jessica M. Gluck   +9 more
wiley   +1 more source

Empagliflozin inhibits Na + /H + exchanger activity in human atrial cardiomyocytes [PDF]

, 2020
Aims Recent clinical trials have proven gliflozins to be cardioprotective in diabetic and non-diabetic patients. However, the underlying mechanisms are incompletely understood.
Lebek, Simon, Sossalla, Samuel T., Riechel, Johannes, Wagner, Stefan, Arzt, Michael, Maier, Lars S., Trum, Maximilian, Pabel, Steffen, Hirt, Stephan   +8 more
core   +1 more source