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Isolation and Culture of Human Keratinocytes
2019Culturing keratinocytes to form coherent epithelial tissue sheets has improved the treatment of extensively burned patients. Keratinocyte culture is also used to investigate various cellular and molecular mechanisms involved in different skin pathologies.
Sergio, Cortez Ghio +4 more
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Characterization of human keratinocyte
International Journal of Cancer, 1981AbstractIntraspecific human cell hybrids were created by fusing normal epidermal keratinocytes with carcinoma (HeLa) cells. All of the hybrids were epithelial in morphology and exhibited a bright cytoskeletal pattern after indirect immunofluorescent labelling by antibody against keratin.
Donna M. Peehl, Eric J. Stanbridge
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Tissue Engineering Part A, 2011
Over the last several years, our in vitro and in vivo studies have focused on optimizing the use of fibrin to deliver cells. We have shown that some three-dimensional (3D) fibrin constructs with specific fibrinogen and thrombin concentration support robust proliferation of normal human dermal fibroblasts, whereas different fibrinogen and thrombin ...
Nadjah, Sese +2 more
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Over the last several years, our in vitro and in vivo studies have focused on optimizing the use of fibrin to deliver cells. We have shown that some three-dimensional (3D) fibrin constructs with specific fibrinogen and thrombin concentration support robust proliferation of normal human dermal fibroblasts, whereas different fibrinogen and thrombin ...
Nadjah, Sese +2 more
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Chloride Transport Pathways in Human Keratinocytes
1991The transport of C1- across cell membrane plays an important role in a number of biological functions, including the maintenance of cell volume and the regulation of intracellular pH. A variety of systems capable of transporting C1- across the plasma membrane have been described in mammalian cells.
Rugolo, M +2 more
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Heat evolution of cultured human keratinocytes
Experientia, 1986The heat production of normal and transformed human epidermal keratinocytes precultured in Petriperm tissue culture dishes was measured calorimetrically. For this purpose, the membrane at the bottom of the culture dish was cut out aseptically and put into a microcalorimeter vessel with the cell layer inwards.
U, Reichert, B, Schaarschmidt
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Protease in Normal Human Epidermal Keratinocytes
Drug and Chemical Toxicology, 1998An in vitro normal human epidermal keratinocytes (NHEK) model was used to study and to characterize the protease stimulated by the mustards 2-chloroethyl ethyl sulphide (CEES), 2-chloro-N-(2-chloroethyl)-N-methylethanamine hydrochloride (nitrogen mustard, HN2), and Bis-2-chloroethyl sulfide (sulfur mustard, HD).
P, Ray, S T, Ali
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Arsenate suppression of human keratinocyte programming
Mutation Research/Reviews in Mutation Research, 1997The human keratinocyte line SCC-9 has been used as a model for arsenate-induced perturbations of differentiation. Growth of these cells in 10 microM arsenate permitted the cultures to reach confluence, but prevented expression of 6 markers of suprabasal differentiation (involucrin, loricrin, filaggrin, spr 1, keratin 1 and keratin 10) as assayed by ...
D J, Kachinskas +3 more
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Insulin receptors in cultured human keratinocytes
British Journal of Dermatology, 1984Insulin binding to receptors was studied for the first time in cultured normal epidermal human keratinocytes. Binding was measured at 16 degrees C in steady-state conditions using 125I-labelled iodohormone and varying concentrations of unlabelled hormone.
P, Verrando, J P, Ortonne
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In-vitro cultivation of human oral keratinocytes
Archives of Oral Biology, 1979Abstract The technique of Rheinwald and Green (1975b) for the serial cultivation of foreskin keratinocytes was modified to facilitate the cultivation of gingival and buccal keratinocytes. We used 7–10-day epithelial outgrowths from excised tissue fragments as a source of keratinocytes, whereas Rheinwald and Green used enzymically-dissociated ...
L, Taichman, S, Reilly, P R, Garant
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Clonal analysis of human clonogenic keratinocytes
2022Regenerative medicine has its roots in harnessing stem cells for permanent restoration of damaged or diseased tissues. The first procedure for the transplantation of epidermal cultures in massive full-thickness burns was established in the 1980s. Since then, epithelial stem cell-based therapies have been further developed in cell and gene therapy ...
Enzo E. +5 more
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