Results 131 to 140 of about 102,593 (165)
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Human keratinocytes express fractalkine/CX3CL1
Journal of Dermatological Science, 2003fractalkine/CX3CL1 is a unique chemokine that has properties of both chemoattractants and adhesion molecules. The major source of this chemokine in the skin is still controversial.studies were undertaken to determine the expression of fractalkine in human skin.RT-PCR, Western blotting, and immunostaining were performed with normal human epidermal ...
Makoto, Sugaya +5 more
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Effect of strain on human keratinocytes in vitro
Journal of Cellular Physiology, 1997Tissue expansion, a technique to enlarge the skin surface area with an expandable balloon, has been widely used in reconstructive surgery. Although the effect of tissue expansion on in vivo skin physiology and histology has been well documented, it remains unclear whether keratinocytes or other cell types are responsible for these changes.
T, Takei +7 more
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Keratins in Cultivated Human Keratinocytes Are Stable*
2015Cultures of human keratinocytes were established according to the technique of Rheinwald and Green. When cultures were exposed to [14C] leucine, the uptake of leucine and increase in the specific activity of 6 urea-extractable polypeptides was prompt-each keratin achieved 50% of its peak specific activity in 3-6 hours.
J, McGuire +3 more
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Tissue culture of human cholesteatomatous keratinocytes
Clinical Otolaryngology, 1983The aim of this study was to culture keratinocytes from migratory skin and from cholesteatomatous matrix. At operation, cholesteatomatous matrix, normal drumhead and non-migratory stratifying skin from the extrameatal incision were obtained. Successful cultures of keratinocytes were produced in 4 out of 5 attempts.
D W, Proops, E K, Parkinson
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Captopril modulates acetylcholinesterase in human keratinocytes
Archives of Dermatological Research, 2011Human keratinocytes synthesize and secrete non-neuronal acetylcholine, which acts as a local cell signaling molecule, regulating functions like proliferation, cell adhesion, motility, desmosomal cell contact, and glandular activity. The keratinocyte acetylcholine axis is composed of the enzymes mediating acetylcholine synthesis (acetyltransferase) and ...
Baroni, A +8 more
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CD40 is functionally expressed on human keratinocytes
European Journal of Immunology, 1996AbstractThe CD40/gp39 pathway is known to be an important feature of B/T cell collaboration leading to T cell‐dependent activation, proliferation or differentiation of B cells. Additionally, CD40 is involved in the regulation of B cell survival and apoptosis.
Denfeld, Ralf W. +9 more
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The presence of h2-calponin in human keratinocyte
Journal of Dermatological Science, 1997Calponin (h1 isoform) was characterized as a smooth muscle specific, actin-, tropomyosin-, calmodulin-binding protein and described as a factor which inhibits contraction. H2-calponin, encoded by a different gene from h1-calponin, was identified from the smooth muscles of mouse and pig. However, non-muscle calponin analogues have recently been reported
Y, Fukui +4 more
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Culture of human keratinocytes and production of keratinocyte grafts in vitro
2003Extensive skin defects after the burn injuries are covered by skin transplants, lyophilized animal skin, artificial tissues made of biodegradable material or the skin epithelial cells (keratinocytes) cultured in vitro. The biotechnological method for the large-scale production of human keratinocytes has been discovered about twenty years ago and today ...
Kraus, Ognjen +4 more
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Preparation of Human Epidermal Keratinocyte Cultures
Current Protocols in Cell Biology, 2003AbstractCultured keratinocytes have been used by a number of investigators in studies investigating wound repair and carcinogenesis, and they have also proven useful as a model for differentiation. This unit describes a protocol for establishing human keratinocytes in tissue culture.
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