Results 41 to 50 of about 59,735 (325)

Development of an In-House Antigen ELISA Using Monoclonal and Polyclonal Antibodies Against an Isolated Canine Distemper Virus. [PDF]

Vet Med Sci
The present work describes the isolation and characterization of CDV from dogs and its molecular confirmation by RT‐PCR targeting the gene encoding the F protein. Sequencing and phylogenetic analysis were undertaken to determine the strain relatedness of the viral genome.
Kökkaya S, Göksu AG.
europepmc   +2 more sources

High-throughput single-cell screening of viable hybridomas and patient-derived antibody-secreting cells using punchable microwells

Artificial Cells, Nanomedicine, and Biotechnology
Monoclonal antibodies (mAbs) hold significant potential as therapeutic agents and are invaluable tools in biomedical research. However, the lack of efficient high-throughput screening methods for single antibody-secreting cells (ASCs) has limited the ...
Kaat Rubben, Ann-Sophie Vander Plaetsen, Ruben Almey, Olivier Tytgat, Koen Deserranno, Jamie Debaere, Delphine Diana Acar, Philip Meuleman, Dieter Deforce, Filip Van Nieuwerburgh   +9 more
doaj   +1 more source

Manufacturing of Hybridomas-Producers of Monoclonal Anti-Bodies to Tularemia Agent Antigens

Проблемы особо опасных инфекций, 2015
Carried out have been two experimental studies on hybridization of mouse myeloma cells and lymphocytes of BALB/c mice immunized with inactivated microbe Francisella tularensis cultures.
O. O. Fomenkov, G. D. Elagin, G. V. Kuklina, D. V. Pechenkin, V. V. Krupin, N. V. Bogacheva, A. A. Kytmanov, A. V. Eremkin, O. V. Tikhvinskaya, E. Yu. Vakhnov   +9 more
doaj   +1 more source

Production and characterization of monoclonal antibodies raised against recombinant human granzymes A and B and showing cross reactions with the natural proteins [PDF]

, 1993
The human serine proteases granzymes A and B are expressed in cytotoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. Recombinant granzyme A and granzyme B proteins were produced in bacteria, purified and then used to raise ...
Borst, Jannie, Brakenhoff, Just P.J., Hack, C. Erik, Kamp, Angela M., Kummer, J. Alain, Radosevic, K., Radosevic, Katarina, van Katwijk, Marcel, van Leeuwen, Anne Marie, Verweij, Cornelis L.   +9 more
core   +4 more sources

Development of a bispecific monoclonal antibody to pesticide carbofuran and triazophos using hybrid hybridomas.

Journal of Food Science, 2009
A mouse hybrid hybridoma (tetradoma) was derived from fusing hybridomas producing monoclonal antibody to N-methylcarbamate pesticide carbofuran with hybridomas producing monoclonal antibody to organophosphorus pesticide Triazophos. The prepared tetradoma
R. Jin, Y. R. Guo, C. M. Wang, J. Wu, G. Zhu   +4 more
semanticscholar   +1 more source

Heterologous screening of hybridomas for the development of broad-specific monoclonal antibodies against deoxynivalenol and its analogues [PDF]

, 2014
Hapten heterology was introduced into the steps of hybridoma selection for the development of monoclonal antibodies (MAbs) against deoxynivalenol (DON).
Bracke, Marc, De Saeger, Sarah, Deforce, Dieter, Eremin, S, Galvita, Anastasia, Guo, Yirong, Heyerick, Arne, Sanders, Melanie   +7 more
core   +1 more source

A New Strategy for Efficient Screening and Identification of Monoclonal Antibodies against Oncogenic Avian Herpesvirus Utilizing CRISPR/Cas9-Based Gene-Editing Technology

Viruses, 2022
Marek’s disease virus (MDV) is an important oncogenic α-herpesvirus that induces Marek’s disease (MD), characterized by severe immunosuppression and rapid-onset T-cell lymphomas in its natural chicken hosts.
Man Teng, Zi-Yu Zhou, Yongxiu Yao, Venugopal Nair, Gai-Ping Zhang, Jun Luo   +5 more
doaj   +1 more source

Retinoic acid induces the differentiation of B cell hybridomas from patients with common variable immunodeficiency. [PDF]

, 1988
Human-human B cell hybridomas constructed from B lymphocytes of common variable immunodeficiency (CVI) patients and the nonsecreting cell line WIL2/729 HF consistently secrete low levels of Ig and appear to retain a defect characteristic of the CVI ...
Adelman, DC, Gilly, M, Saxon, A, Sherr, E, Sidell, N, Wall, R   +5 more
core  

Hybridoma Technology

, 2022
Hybridoma technology combines the unique qualities of antibody-producing B cells with immortalized myeloma cells, creating hybrid cells capable of endless proliferation while retaining the ability to produce specific antibodies. The process begins with the immunization of a host animal, typically a mouse, followed by the fusion of its spleen cells ...
openaire   +2 more sources

Two better cell lines for making hybridomas expressing specific T cell receptors.

Journal of Immunology, 1989
Two variants of the AKR thymoma BW5147 have been isolated which can no longer express functional TCR alpha- and beta-chains. By generating hybridomas with these variant fusion lines, TCR of any normal T lymphocyte, including TCR-gamma/delta, can be ...
Janicé White, M. Blackman, J. Bill, J. Kappler, P. Marrack, D. Gold, W. Born   +6 more
semanticscholar   +1 more source