Results 41 to 50 of about 59,454 (330)

Fluorescent Microtiter Screening Assay for Immunocytochemically Reactive Antibodies

BioTechniques, 1997
We have developed a rapid in situ screening procedure that enables prescreening of hundreds of hybridomas in a 96-well format. The procedure involves fluorescence immunostaining of cells cultured in 96-well plates and the use of a fluorescence plate ...
J.-L. Su, K.E. Kilpatrick, B.R. Champion, D.C. Morris, J.M. Lehmann, T.A. Kost   +5 more
doaj   +1 more source

A hybridoma-derived monoclonal antibody with high homology to the aberrant myeloma light chain.

PLoS ONE, 2021
The identification of antibody variable regions in the heavy (VH) and light (VL) chains from hybridomas is necessary for the production of recombinant, sequence-defined monoclonal antibodies (mAbs) and antibody derivatives.
Ghasidit Pornnoppadol, Boya Zhang, Alec A Desai, Anthony Berardi, Henriette A Remmer, Peter M Tessier, Colin F Greineder   +6 more
doaj   +1 more source

Manufacturing of Hybridomas-Producers of Monoclonal Anti-Bodies to Tularemia Agent Antigens

Проблемы особо опасных инфекций, 2015
Carried out have been two experimental studies on hybridization of mouse myeloma cells and lymphocytes of BALB/c mice immunized with inactivated microbe Francisella tularensis cultures.
O. O. Fomenkov, G. D. Elagin, G. V. Kuklina, D. V. Pechenkin, V. V. Krupin, N. V. Bogacheva, A. A. Kytmanov, A. V. Eremkin, O. V. Tikhvinskaya, E. Yu. Vakhnov   +9 more
doaj   +1 more source

Characterization and epitope mapping of human monoclonal antibodies to PDC-E2, the immunodominant autoantigen of primary biliary cirrhosis [PDF]

, 1992
Further to define the epitopes of PDC-E2, the major autoantigen in primary biliary cirrhosis (PBC), we have developed and characterized five human monoclonal antibodies. These antibodies were derived by fusing a regional hepatic lymph node from a patient
Bassendine, Baum, Casali, Chambers, Coppel, Coppel, Cram, Flannery, Fregeau, Fregeau, Fujikura, Fusey, Fussey, Gershwin, Gershwin, Griffin, Griffin, James, Kaplan, Kunicki, Larrick, Leung, Leung, Mackay, Mackay, Mathews, McNeilage, Okamoto, Pollack, Ramsden, Reeves, Reimer, Robbins, Rokeach, Rowley, Ruther, Salemuddin, Sturgess, Sudin, Surh, Surh, Surh, Surh, Tan, Uibo, Van de Water, Van de Water, Van de Water, Van de Water, Williams   +49 more
core   +1 more source

Development of a bispecific monoclonal antibody to pesticide carbofuran and triazophos using hybrid hybridomas.

Journal of Food Science, 2009
A mouse hybrid hybridoma (tetradoma) was derived from fusing hybridomas producing monoclonal antibody to N-methylcarbamate pesticide carbofuran with hybridomas producing monoclonal antibody to organophosphorus pesticide Triazophos. The prepared tetradoma
R. Jin, Y. R. Guo, C. M. Wang, J. Wu, G. Zhu   +4 more
semanticscholar   +1 more source

High-throughput single-cell screening of viable hybridomas and patient-derived antibody-secreting cells using punchable microwells

Artificial Cells, Nanomedicine, and Biotechnology
Monoclonal antibodies (mAbs) hold significant potential as therapeutic agents and are invaluable tools in biomedical research. However, the lack of efficient high-throughput screening methods for single antibody-secreting cells (ASCs) has limited the ...
Kaat Rubben, Ann-Sophie Vander Plaetsen, Ruben Almey, Olivier Tytgat, Koen Deserranno, Jamie Debaere, Delphine Diana Acar, Philip Meuleman, Dieter Deforce, Filip Van Nieuwerburgh   +9 more
doaj   +1 more source

Retinoic acid induces the differentiation of B cell hybridomas from patients with common variable immunodeficiency. [PDF]

, 1988
Human-human B cell hybridomas constructed from B lymphocytes of common variable immunodeficiency (CVI) patients and the nonsecreting cell line WIL2/729 HF consistently secrete low levels of Ig and appear to retain a defect characteristic of the CVI ...
Adelman, DC, Gilly, M, Saxon, A, Sherr, E, Sidell, N, Wall, R   +5 more
core  

Two minor determinants of myelin basic protein induce experimental allergic encephalomyelitis in SJL/J mice [PDF]

, 1988
Experimental allergic encephalomyelitis (EAE)' is an autoimmune inflammatory demyelinating disease in the central nervous system (CNS) of animals immunized with myelin basic protein (MBP). The disease is directly mediated by Thelper cells that recognize
Ando, Dale G., Hood, Leroy, Horvath, Suzanna J., Kono, Dwight H., Saavedra, Raul A., Urban, James L.   +5 more
core   +1 more source

Two better cell lines for making hybridomas expressing specific T cell receptors.

Journal of Immunology, 1989
Two variants of the AKR thymoma BW5147 have been isolated which can no longer express functional TCR alpha- and beta-chains. By generating hybridomas with these variant fusion lines, TCR of any normal T lymphocyte, including TCR-gamma/delta, can be ...
Janicé White, M. Blackman, J. Bill, J. Kappler, P. Marrack, D. Gold, W. Born   +6 more
semanticscholar   +1 more source

ShcD adaptor protein drives invasion of triple negative breast cancer cells by aberrant activation of EGFR signaling

Molecular Oncology, EarlyView.
We identified adaptor protein ShcD as upregulated in triple‐negative breast cancer and found its expression to be correlated with reduced patient survival and increased invasion in cell models. Using a proteomic screen, we identified novel ShcD binding partners involved in EGFR signaling pathways.
Hayley R. Lau, Hayley S. Smith, Begüm Alural, Claire E. Martin, Laura A. New, Manali Tilak, Sara L. Banerjee, Hannah N. Robeson, Nicolas Bisson, Anne‐Claude Gingras, Jasmin Lalonde, Nina Jones   +11 more
wiley   +1 more source