Results 71 to 80 of about 59,735 (325)

Hybridoma technology: is it still useful?

Current Research in Immunology, 2021
The isolation of single monoclonal antibodies (mAbs) against a given antigen was only possible with the introduction of the hybridoma technology, which is based on the fusion of specific B lymphocytes with myeloma cells. Since then, several mAbs were described for therapeutic, diagnostic, and research purposes.
Jane Zveiter Moraes, Bárbara Hamaguchi, Camila Braggion, Enzo Reina Speciale, Fernanda Beatriz Viana Cesar, Gabriela de Fátima da Silva Soares, Juliana Harumi Osaki, Tauane Mathias Pereira, Rodrigo Barbosa Aguiar   +8 more
openaire   +4 more sources

Glucose‐Responsive PAGR1‐Regulated Skeletal Muscle Gene Program Controls Systemic Glucose Homeostasis and Hepatic Metabolism

Advanced Science, EarlyView.
The study reveals that PAXIP1‐associated glutamate‐rich protein 1 (PAGR1), a glucose‐responsive regulator in skeletal muscle, modulates systemic glucose homeostasis and hepatic metabolism. Muscle‐specific PAGR1 deletion enhances insulin signaling, promotes glucose transporter 4 (GLUT4) translocation, and protects against high‐fat‐diet‐induced insulin ...
Chenyun Ding, Yuhuan Jia, Lin Liu, Wen Wang, Danxia Zhou, Zheng Zhou, Likun Yang, Xinyi Chen, Di Chen, Yan Mao, Liwei Xiao, Cai‐Zhi Liu, Zhen‐Yu Du, Yujing Yin, Qiqi Guo, Zongchao Sun, Kai Ge, Tingting Fu, Hai‐Long Piao, Zhenji Gan   +19 more
wiley   +1 more source

Characterization of B Cell Responses in Rainbow Trout (Oncorhynchus Mykiss) Affected by Red Mark Syndrome

Advanced Science, EarlyView.
This study extensively characterizes the B cell response of rainbow trout (Oncorhynchus mykiss) in response to red mark syndrome (RMS). This disease, suspected to be caused by a Midichloria‐like organism (MLO), induces skin lesions in the infected animals, characterized by a massive influx of IgM+ B cells.
J Germán Herranz‐Jusdado, Samuel Vicente‐Gil, Esther Morel, Pablo Jiménez‐Barrios, Rocío Simón, Pedro Perdiguero, Diana Martín, Marta Vargas‐Ramírez, Niels Lorenzen, Jacob Günther Schmidt, Carolina Tafalla   +10 more
wiley   +1 more source

Variable major proteins of Borrelia hermsii. Epitope mapping and partial sequence analysis of CNBr peptides. [PDF]

, 1985
The variable major proteins (VMP) of serotypes 7 and 21 of the relapsing fever agent Borrelia hermsii were isolated by detergent extraction and high performance liquid chromatography.
Barbour, AG, Barstad, PA, Coligan, JE, Raum, MG   +3 more
core  

Definition of Drosophila hemocyte subsets by cell-type specific antigens. [PDF]

, 2007
We analyzed the heterogeneity of Drosophila hemocytes on the basis of the expression of cell-type specific antigens. The antigens characterize distinct subsets which partially overlap with those defined by morphological criteria.
Andó, István, Csorba, Kinga, Gateff, Elisabeth, Hultmark, Dan, Kurucz, Éva, Laurinyecz, Barbara, Márkus, Róbert, Vilmos, Péter, Váczi, Balázs, Zsámboki, János   +9 more
core   +1 more source

Rabbit monoclonal antibodies: generating a fusion partner to produce rabbit-rabbit hybridomas.

Proceedings of the National Academy of Sciences of the United States of America, 1995
During the last 15 years several laboratories have attempted to generate rabbit monoclonal antibodies, mainly because rabbits recognize antigens and epitopes that are not immunogenic in mice or rats, two species from which monoclonal antibodies are ...
H. Spieker-Polet, P. Sethupathi, P. Yam, K. Knight   +3 more
semanticscholar   +1 more source

Large‐Scale Production of Transfusion‐Ready Red Blood Cells From Induced Pluripotent Stem Cells

Advanced Science, EarlyView.
A scalable, dynamic culture system is developed to produce red blood cells from induced pluripotent stem cells. It achieves high yields with up to 70% enucleation, not previously shown in dynamic systems, overcoming key limitations and enabling large‐scale production.
Eszter Varga, Eelke Brandsma, Brenda E. Juarez‐Garza, Renuka P. E. Ramlal, Julien J. Karrich, Adrien Laurent, Athina Chavli, Ruthmila Paskel, Kerly Fu, Richard A. Flavell, Marieke von Lindern, Derk Amsen, Marieke E. Klijn, Emile van den Akker   +13 more
wiley   +1 more source

Positive selection determines T cell receptor V beta 14 gene usage by CD8+ T cells. [PDF]

, 1989
We report here a mAb, 14-2, reactive with TCRs that include V beta 14. The frequency of V beta 14+ T cells varies with CD4 and CD8 subset and is controlled by the H-2 genes.
Liao, N, Maltzman, J, RAULET, David H.
core  

T cell receptor-dependent cell death of T cell hybridomas mediated by the CD30 cytoplasmic domain in association with tumor necrosis factor receptor-associated factors

Journal of Experimental Medicine, 1996
CD30 is a member of the tumor necrosis factor superfamily and a surface marker for Hodgkin's disease. Normal activated T cells and several virally transformed T or B cell lines also show CD30 expression.
Soo-Young Lee, Chae Gyu Park, Yong-Sun Choi   +2 more
semanticscholar   +1 more source

Identification Epitope on VP4 Protein of Senecavirus A Recognized by a Monoclonal Antibody

Animal Research and One Health, EarlyView.
This study aims to identify conserved antigenic epitopes on the SVA VP4 protein using monoclonal antibodies, providing valuable tools for further exploring its functions and developing detection methods. SVA VP4 protein was expressed in Escherichia coli and purified with High‐Affinity Ni‐Charged Resin FF.
Liang Meng, Xuyan Xiang, Xinyu Qi, Hanrong Zhou, Peiyu Xiao, Fandan Meng, Tongqing An, Haiwei Wang   +7 more
wiley   +1 more source