Results 31 to 40 of about 141,525 (260)

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Probing the active site of Class 3 L-asparaginase by mutagenesis. I. Tinkering with the zinc coordination site of ReAV

Frontiers in Chemistry
ReAV, the inducible Class-3 L-asparaginase from the nitrogen-fixing symbiotic bacterium Rhizobium etli, is an interesting candidate for optimizing its enzymatic potential for antileukemic applications.
Kinga Pokrywka, Marta Grzechowiak, Joanna Sliwiak, Paulina Worsztynowicz, Joanna I. Loch, Milosz Ruszkowski, Miroslaw Gilski, Miroslaw Gilski, Mariusz Jaskolski, Mariusz Jaskolski   +9 more
doaj   +1 more source

Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil

Toxins, 2022
The kinetics and thermodynamics of the enzymatic degradation of zearalenone (ZEN) in degummed corn oil were investigated by analyzing the impacts of temperature, pH, ZEN hydrolase dosage and ZEN concentration on the initial reaction rate.
Chenwei Zhao, Pengkai Xie, Jun Jin, Qingzhe Jin, Xingguo Wang   +4 more
doaj   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Construction of an engineered Bacillus subtilis for production of poly-γ-glutamic acids with specific molecular weights

Frontiers in Microbiology
IntroductionPoly-γ-glutamic acid (γ-PGA) with different molecular weight (Mw) exhibits different properties and therefore has a variety of applications. At present, the γ-PGA is mainly produced by Bacillus species.
Yu Lin, Yiwei Ding, Lin Shu, Lin Shu, Huizhen Chen, Nan Li, Xiaoqun Duan, Wei Zeng, Wei Zeng, Wei Zeng   +9 more
doaj   +1 more source

Isolation and Determination of Bile Salt Hydrolase-Producing Lactic Acid Bacteria from Fermented Spider Plant

Journal of Pure and Applied Microbiology, 2018
Bile salt hydrolase is an enzyme from bacteria that can hydrolyze bile acid to be insoluble form and this event lead to reducing of cholesterol level in serum.
Atipat Yasiri, Emilie Vannaxay, Jinatta Kiatmontri, Supawadee Seubsasana   +3 more
doaj   +1 more source

Enzymatic Degradation of Zearalenone in the Gastrointestinal Tract of Pigs, Chickens, and Rainbow Trout

Toxins, 2023
The estrogenic mycotoxin zearalenone (ZEN) is a common contaminant of animal feed. Effective strategies for the inactivation of ZEN in feed are required.
Christiane Gruber-Dorninger, Manuela Killinger, Andreas Höbartner-Gußl, Roy Rosen, Barbara Doupovec, Markus Aleschko, Heidi Schwartz-Zimmermann, Oliver Greitbauer, Zoran Marković, Marko Stanković, Karin Schöndorfer, Djuro Vukmirovic, Silvia Wein, Dian Schatzmayr   +13 more
doaj   +1 more source

Anticancer sensitivities and biological characteristics of HCT116 cells resistant to the selective poly(ADP‐ribose) glycohydrolase inhibitor

FEBS Open Bio, EarlyView.
We analyzed alterations of PAR metabolism‐related proteins in PARG inhibitor‐resistant HCT116RPDD cells. Although PARG levels remained unchanged, HCT116RPDD cells exhibited reduced PARP1 and ARH3 expression and elevated PAR levels. Interestingly, HCT116RPDD cells exhibited slightly elevated intracellular NAD+/NADH and ATP levels. Our findings suggest a
Kaede Tsuda, Yoko Ogino, Akira Sato
wiley   +1 more source

Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles

Sensors, 2014
Proteinases are involved in a multitude of important physiological processes, such as protein metabolism. For this reason, a conductometric enzyme biosensor based on proteinase K was developed using two types of nanoparticles (gold and magnetic).
Wided Nouira, Abderrazak Maaref, Hamid Elaissari, Francis Vocanson, Maryam Siadat, Nicole Jaffrezic-Renault   +5 more
doaj   +1 more source

New Insights Into Human Hyaluronidase 4/Chondroitin Sulphate Hydrolase

Frontiers in Cell and Developmental Biology, 2021
In this review, the current experimental evidence, literature and hypotheses surrounding hyaluronidase 4 [HYAL4, also known as chondroitin sulphate hydrolase (CHSE)] and chondroitin sulphate (CS) are explored. Originally named for its sequence similarity
Marissa L. Maciej-Hulme
doaj   +1 more source