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Human Hypoxanthine Phosphoribosyltransferase [PDF]

Journal of Biological Chemistry, 1969
Hypoxanthine phosphoribosyltransferase (inosine monophosphate:pyrophosphate phosphoribosyltransferase EC 2.4.2.8) has been purified 50-fold from an acid-treated lysate of human erythrocytes. The average particle weight of the enzyme was estimated by gel filtration at 60,000.
Thomas A. Krenitsky, R Papaioannou, Gertrude B. Elion   +2 more
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Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells [PDF]

Molecular Therapy: Nucleic Acids, 2016
Urea cycle disorders are incurable enzymopathies that affect nitrogen metabolism and typically lead to hyperammonemia. Arginase deficiency results from a mutation in Arg1, the enzyme regulating the final step of ureagenesis and typically results in ...
Patrick C Lee, Brian Truong, Agustin Vega-Crespo, W Blake Gilmore, Kip Hermann, Stephanie AK Angarita, Jonathan K Tang, Katherine M Chang, Austin E Wininger, Alex K Lam, Benjamen E Schoenberg, Stephen D Cederbaum, April D Pyle, James A Byrne, Gerald S Lipshutz   +14 more
doaj   +5 more sources

Prioritization of Deleterious Variations in the Human Hypoxanthine-Guanine Phosphoribosyltransferase Gene [PDF]

Medical Laboratory Journal, 2018
Background and Objectives: Non-synonymous single nucleotide polymorphisms are typical genetic variations that may potentially affect the structure or function of expressed proteins, and therefore could be involved in complex disorders.
Semira Kheiri, Mahdieh Safarzad, Mohammad Shariati, Hoda Sohrabi   +3 more
doaj   +2 more sources

Purification and characterization of mouse hypoxanthine-guanine phosphoribosyltransferase.

Journal of Biological Chemistry, 1975
Hypoxanthine-guanine phosphoribosyltransferase (HGPR transferase) (EC 2.4.2.8) has been purified approximately 4500-fold to apparent homogeneity from mouse liver. The procedure involves the use of affinity chromatography and was designed to be readily adaptable to small scale isolations.
Stephen H. Hughes, Geoffrey M. Wahl, Mario R. Capecchi   +2 more
openalex   +4 more sources

Kinetic Studies of Hypoxanthine-Guanine Phosphoribosyltransferase

Journal of Biological Chemistry, 1968
Abstract The mechanism of reaction of human erythrocyte hypoxanthine-guanine phosphoribosyltransferase was investigated by initial velocity, product inhibition, and isotope exchange studies. Although initial velocity data are compatible with a mechanism involving binary enzyme-substrate complexes, the product inhibition and isotope exchange studies ...
Jane Henderson, Larry W. Brox, W. N. Kelley, F M Rosenbloom, J. Edwin Seegmiller   +4 more
openalex   +4 more sources

Crystal structure of Leishmania tarentolae hypoxanthine-guanine phosphoribosyltransferase [PDF]

BMC Structural Biology, 2007
Abstract Background Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) (EC 2.4.2.8) is a central enzyme in the purine recycling pathway. Parasitic protozoa of the order Kinetoplastida cannot synthesize purines de novo and use the salvage pathway to synthesize purine bases, making this an attractive target for ...
Paulo Sérgio Monzani, Stefano Trapani, Otávio Henrique Thiemann, Glaucius Oliva   +3 more
openalex   +5 more sources

Human Hypoxanthine-Guanine Phosphoribosyltransferase

Journal of Biological Chemistry, 1971
Abstract Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) has been purified to homogeneity from human erythrocytes obtained from one male donor. The normal human enzyme has a Stokes radius of 36 A with a molecular weight of 68,000 and is composed of two subunits which have identical molecular weight and net charge.
William J. Arnold, William N. Kelley
openalex   +4 more sources

Hypoxanthine-Guanine Phosphoribosyltransferase Is Dispensable for Mycobacterium smegmatis Viability

Journal of Bacteriology, 2019
Purine bases, released by the hydrolytic and phosphorolytic degradation of nucleic acids and nucleotides, can be salvaged and recycled. The hypoxanthine-guanine phosphoribosyltransferase (HGPRT), which catalyzes the formation of guanosine-5′-monophosphate from guanine and inosine-5′-monophosphate from hypoxanthine, represents a potential ...
Zdeněk Knejzlı́k, Klára Herkommerová, Dana Hocková, Iva Pichová   +3 more
openalex   +6 more sources

The Effect of Two Weeks of High-intensity Interval Training on Salvage nucleotide pathway [PDF]

فیزیولوژی ورزش و فعالیت بدنی, 2022
Purpose: The effects of exercise adaptation on improving the purine nucleotides components are inevitable, and physical activity improves the purine nucleotide salvage pathway.
Abbas Ghanbari Niaki, Rouhollah Haghshenas Gatabi   +1 more
doaj   +1 more source

Potential molecular link between the β-amyloid precursor protein (APP) and hypoxanthine-guanine phosphoribosyltransferase (HGprt) enzyme in Lesch-Nyhan disease and cancer

AIMS Neuroscience, 2021
Lesch-Nyhan disease (LND) is a rare X-linked inherited neurogenetic disorders of purine metabolic in which the cytoplasmic enzyme, hypoxanthine-guanine phosphoribosyltransferase (HGprt) is defective.
Khue Vu Nguyen
doaj   +1 more source