Results 51 to 60 of about 13,871 (203)

Falling from grace: HPRT is not suitable as an endogenous control for cancer-related studies

Molecular & Cellular Oncology, 2019
HPRT is a housekeeping enzyme involved in recycling guanine and inosine in the purine salvage pathway. As a housekeeping gene, HPRT has been widely used as an endogenous control for molecular studies evaluating changes in gene expression.
Michelle H. Townsend, Abigail M. Felsted, Zac E. Ence, Stephen R. Piccolo, Richard A. Robison, Kim L. O’Neill   +5 more
doaj   +1 more source

Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells

Molecular Therapy: Nucleic Acids, 2016
Urea cycle disorders are incurable enzymopathies that affect nitrogen metabolism and typically lead to hyperammonemia. Arginase deficiency results from a mutation in Arg1, the enzyme regulating the final step of ureagenesis and typically results in ...
Patrick C Lee, Brian Truong, Agustin Vega-Crespo, W Blake Gilmore, Kip Hermann, Stephanie AK Angarita, Jonathan K Tang, Katherine M Chang, Austin E Wininger, Alex K Lam, Benjamen E Schoenberg, Stephen D Cederbaum, April D Pyle, James A Byrne, Gerald S Lipshutz   +14 more
doaj   +1 more source

Downregulation of NAD Kinase Expression in β‐Cells Contributes to the Aging‐Associated Decline in Glucose‐Stimulated Insulin Secretion

Aging Cell, Volume 24, Issue 4, April 2025.
Adequate NADK/NADK2 level is essential to the proper functioning of redox and folate cycles and the purine biosynthesis pathway, and as well as maintenance of GSIS response. During aging, diminished NADK/NADK2 expression alters the metabolic status quo, increasing AICAR production. The latter activates AMPK and suppresses GSIS.
Guan‐Jie Li, Mei‐Ling Cheng, Yu‐Ting Lin, Yu‐Hsuan Ho, Gigin Lin, Chih‐Yung Chiu, Hung‐Yao Ho   +6 more
wiley   +1 more source

The housekeeping gene hypoxanthine guanine phosphoribosyltransferase (HPRT) regulates multiple developmental and metabolic pathways of murine embryonic stem cell neuronal differentiation. [PDF]

PLoS ONE, 2013
The mechanisms by which mutations of the purinergic housekeeping gene hypoxanthine guanine phosphoribosyltransferase (HPRT) cause the severe neurodevelopmental Lesch Nyhan Disease (LND) are poorly understood.
Tae Hyuk Kang, Yongjin Park, Joel S Bader, Theodore Friedmann   +3 more
doaj   +1 more source

Role for the liver X receptor agonist 22‐ketositosterol in preventing disease progression in an Alzheimer's disease mouse model

British Journal of Pharmacology, EarlyView.
Background and Purpose Liver X receptors (LXRs) are promising therapeutic targets for alleviating Alzheimer's disease (AD) symptoms. We assessed the impact of the semi‐synthetic LXR agonist 22‐ketositosterol on disease progression in an AD mouse model.
Nikita Martens, Na Zhan, Sammie C. Yam, Marcella Palumbo, Lorenzo Pontini, Frank P. J. Leijten, Leonie van Vark‐van der Zee, Gardi Voortman, Silvia Friedrichs, Albert Gerding, Maura Marinozzi, Johan W. Jonker, Folkert Kuipers, Dieter Lütjohann, Tim Vanmierlo, Monique T. Mulder   +15 more
wiley   +1 more source

Modulation of host glutamine anabolism enhances the sensitivity of small cell lung cancer to chemotherapy

Cell Reports, 2023
Summary: Small cell lung cancer (SCLC) is one of the deadliest human cancers, with a 5-year survival rate of ∼7%. Here, we performed a targeted proteomics analysis of human SCLC samples and thereby identified hypoxanthine phosphoribosyltransferase 1 ...
Manabu Kodama, Gouji Toyokawa, Osamu Sugahara, Shigeaki Sugiyama, Naoki Haratake, Yuichi Yamada, Reona Wada, Shinkichi Takamori, Mototsugu Shimokawa, Tomoyoshi Takenaka, Tetsuzo Tagawa, Hiroki Kittaka, Takeshi Tsuruda, Kentaro Tanaka, Yushiro Komatsu, Keisuke Nakata, Yuri Imado, Koji Yamazaki, Isamu Okamoto, Yoshinao Oda, Masatomo Takahashi, Yoshihiro Izumi, Takeshi Bamba, Hideyuki Shimizu, Tomoharu Yoshizumi, Keiichi I. Nakayama   +25 more
doaj  

Unlocking the power of immune checkpoint inhibitors: Targeting YAP1 reduces anti‐PD1 resistance in skin cutaneous melanoma

British Journal of Pharmacology, EarlyView.
Abstract Background and Purpose Immune checkpoint inhibitors, such as anti­PD1, revolutionized melanoma treatment. However, resistance and low response rates remain problems. Our goal was to pinpoint actionable biomarkers of resistance to anti‐PD1 treatment and verify therapeutic effectiveness in vivo.
Szonja Anna Kovács, Tamás Kovács, András Lánczky, Ágnes Paál, Zsombor I. Hegedűs, Nabil V. Sayour, Lilla Szabó, Andrea Kovács, Giampaolo Bianchini, Péter Ferdinandy, Alberto Ocana, Zoltán V. Varga, János Tibor Fekete, Balázs Győrffy   +13 more
wiley   +1 more source

Cardioprotective effects of the extracellular chaperone clusterin in acute myocardial infarction

European Journal of Clinical Investigation, EarlyView.
In vivo, CLU−/− mice were more susceptible to acute myocardial infarction than CLU+/+ mice. Moreover, inflammation and histone levels were significantly increased in CLU−/− mice compared to CLU+/+ mice following ischemia/reperfusion. In vitro, CLU protects cardiomyocytes from histone‐ and/or hypoxia‐induced cell death.
Louwana Allawa, Antoine Poirier, Pascale Pignon, Justine Beaumont, Lucie Lebeau, Agnès Barbelivien, Thomas Bochaton, Céline Beauvillain, Daniel Henrion, Yves Delneste, Pascale Jeannin, Fabrice Prunier, Sophie Tamareille   +12 more
wiley   +1 more source

Harnessing nucleotide metabolism and immunity in cancer: a tumour microenvironment perspective

The FEBS Journal, Volume 292, Issue 9, Page 2155-2172, May 2025.
This review explores how changes in nucleotide metabolism affect the tumour microenvironment and immune responses in cancer. Here, we discuss the dual role of nucleotide metabolism in promoting cancer cell growth and shaping immune suppression. We highlight how therapies targeting nucleotide pathways can enhance immunotherapy efficacy, offering new ...
Hadil Suleiman, Alexandra Emerson, Peter M. Wilson, Karl A. Mulligan, Robert D. Ladner, Melissa J. LaBonte   +5 more
wiley   +1 more source

Evaluation of the upregulation and surface expression of hypoxanthine guanine phosphoribosyltransferase in acute lymphoblastic leukemia and Burkitt’s B cell lymphoma

Cancer Cell International, 2020
Background The aim of this study is to determine whether Hypoxanthine Guanine Phosphoribosyltransferase (HPRT) could be used as a biomarker for the diagnosis and treatment of B cell malignancies.
Michelle H. Townsend, Zac E. Ence, Taylor P. Cox, John E. Lattin, Weston Burrup, Michael K. Boyer, Stephen R. Piccolo, Richard A. Robison, Kim L. O’Neill   +8 more
doaj   +1 more source