Comparison of SARS-CoV-2 sequencing using the ONT GridION and the Illumina MiSeq [PDF]
BMC Genomics, 2022 Background Over 4 million SARS-CoV-2 genomes have been sequenced globally in the past 2 years. This has been crucial in elucidating transmission chains within communities, the development of new diagnostic methods, vaccines, and antivirals.
Derek Tshiabuila +12 more
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Gut microbiome profiling of neonates using Nanopore MinION and Illumina MiSeq sequencing [PDF]
Frontiers in Microbiology, 2023 This study aimed to evaluate the difference in gut microbiomes between preterm and term infants using third-generation long-read sequencing (Oxford Nanopore Technologies, ONT) compared with an established gold standard, Illumina (second-generation short ...
Teahyen Cha +8 more
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Perspectives of using Illumina MiSeq for identification of arbuscular mycorrhizal fungi [PDF]
Вавиловский журнал генетики и селекции, 2020 Arbuscular mycorrhiza fungi (AMF) form one of the most common symbiosis with the majority of land plants. AMF supply the plant with various mineral elements, primarily phosphorus, and improve the water supply.
A. A. Kryukov +6 more
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High-quality single amplicon sequencing method for illumina MiSeq platform using pool of ‘N’ (0–10) spacer-linked target specific primers without PhiX spike-in [PDF]
BMC Genomics, 2023 Background Illumina sequencing platform requires base diversity in the initial 11 cycles for efficient cluster identification and colour matrix estimation. This limitation yields low-quality data for amplicon libraries having homogeneous base composition.
Tejali Naik +4 more
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Illumina MiSeq sequencing analysis of fungal diversity in stored dates [PDF]
BMC Microbiology, 2017 Background Date palm has been a major fruit tree in the Middle East over thousands of years, especially in the Arabian Peninsula. Dates are consumed fresh (Rutab) or after partial drying and storage (Tamar) during off-season.
Ismail M. Al-Bulushi +4 more
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Assessment of seawater bacterial infection in rabbit tibia by Illumina MiSeq sequencing and bacterial culture [PDF]
Journal of Orthopaedic Surgery and Research, 2021 Objectives We aimed to explore the bacterial community composition following ocean bacterial infection using an animal model. Methods This animal-based experiment was conducted from September 2019 to November 2019. Eighteen seawater filter membranes were
Du Wang +6 more
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Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform. [PDF]
PLoS ONE, 2017 Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist.
Chongqing Wen +10 more
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Quasispecies Analyses of the HIV-1 Near-full-length Genome with Illumina MiSeq [PDF]
Frontiers in Microbiology, 2015 Human immunodeficiency virus type-1 (HIV-1) exhibits high between-host genetic diversity and within-host heterogeneity, recognized as quasispecies. Because HIV-1 quasispecies fluctuate in terms of multiple factors, such as antiretroviral exposure and ...
Hirotaka eOde +10 more
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Illumina MiSeq Sequencing for Preliminary Analysis of Microbiome Causing Primary Endodontic Infections in Egypt [PDF]
International Journal of Microbiology, 2018 The use of high throughput next generation technologies has allowed more comprehensive analysis than traditional Sanger sequencing. The specific aim of this study was to investigate the microbial diversity of primary endodontic infections using Illumina ...
Sally Ali Tawfik +3 more
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Saliva microbiome profiling by full-gene 16S rRNA Oxford Nanopore Technology versus Illumina MiSeq sequencing [PDF]
npj Biofilms and MicrobiomesMolecular characterization of the oral microbiome is a crucial first step in experiments which aim to understand the complex dynamics of the oral microbiome or the interplay with host health and disease.
Anders Esberg +3 more
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