Gut microbiome profiling of neonates using Nanopore MinION and Illumina MiSeq sequencing [PDF]
Frontiers in Microbiology, 2023 This study aimed to evaluate the difference in gut microbiomes between preterm and term infants using third-generation long-read sequencing (Oxford Nanopore Technologies, ONT) compared with an established gold standard, Illumina (second-generation short ...
Teahyen Cha +8 more
doaj +5 more sources
Perspectives of using Illumina MiSeq for identification of arbuscular mycorrhizal fungi [PDF]
Вавиловский журнал генетики и селекции, 2020 Arbuscular mycorrhiza fungi (AMF) form one of the most common symbiosis with the majority of land plants. AMF supply the plant with various mineral elements, primarily phosphorus, and improve the water supply.
A. A. Kryukov +6 more
doaj +6 more sources
Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform. [PDF]
PLoS ONE, 2017 Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist.
Chongqing Wen +10 more
doaj +8 more sources
Comparison of SARS-CoV-2 sequencing using the ONT GridION and the Illumina MiSeq [PDF]
BMC Genomics, 2022 Background Over 4 million SARS-CoV-2 genomes have been sequenced globally in the past 2 years. This has been crucial in elucidating transmission chains within communities, the development of new diagnostic methods, vaccines, and antivirals.
Derek Tshiabuila +12 more
doaj +4 more sources
High-quality single amplicon sequencing method for illumina MiSeq platform using pool of ‘N’ (0–10) spacer-linked target specific primers without PhiX spike-in [PDF]
BMC Genomics, 2023 Background Illumina sequencing platform requires base diversity in the initial 11 cycles for efficient cluster identification and colour matrix estimation. This limitation yields low-quality data for amplicon libraries having homogeneous base composition.
Tejali Naik +4 more
doaj +3 more sources
Illumina MiSeq sequencing analysis of fungal diversity in stored dates [PDF]
BMC Microbiology, 2017 Background Date palm has been a major fruit tree in the Middle East over thousands of years, especially in the Arabian Peninsula. Dates are consumed fresh (Rutab) or after partial drying and storage (Tamar) during off-season.
Ismail M. Al-Bulushi +4 more
doaj +5 more sources
Saliva microbiome profiling by full-gene 16S rRNA Oxford Nanopore Technology versus Illumina MiSeq sequencing [PDF]
npj Biofilms and MicrobiomesMolecular characterization of the oral microbiome is a crucial first step in experiments which aim to understand the complex dynamics of the oral microbiome or the interplay with host health and disease.
Anders Esberg +3 more
doaj +3 more sources
Quasispecies Analyses of the HIV-1 Near-full-length Genome with Illumina MiSeq [PDF]
Frontiers in Microbiology, 2015 Human immunodeficiency virus type-1 (HIV-1) exhibits high between-host genetic diversity and within-host heterogeneity, recognized as quasispecies. Because HIV-1 quasispecies fluctuate in terms of multiple factors, such as antiretroviral exposure and ...
Hirotaka eOde +10 more
doaj +5 more sources
Comparison of Oxford Nanopore Technologies and Illumina MiSeq sequencing with mock communities and agricultural soil. [PDF]
Sci Rep, 2023 Illumina MiSeq is the current standard for characterizing microbial communities in soil. The newer alternative, Oxford Nanopore Technologies MinION sequencer, is quickly gaining popularity because of the low initial cost and longer sequence reads ...
Stevens BM +3 more
europepmc +2 more sources
A5-miseq: an updated pipeline to assemble microbial genomes from Illumina MiSeq data [PDF]
Bioinformatics, 2014 MOTIVATION Open-source bacterial genome assembly remains inaccessible to many biologists because of its complexity. Few software solutions exist that are capable of automating all steps in the process of de novo genome assembly from Illumina data ...
D. Coil, G. Jospin, A. Darling
semanticscholar +5 more sources