Results 21 to 30 of about 156,271 (269)

Design and use of a new primer pair for the characterization of the cyanobacteria Synechococcus and Prochlorococcus communities targeting petB gene through metabarcoding approaches

open access: yesMethodsX, 2023
During the last years, the application of next-generation sequencing (NGS) technologies to search for specific genetic markers has become a crucial method for the characterization of microbial communities.
Alexandra Coello-Camba   +2 more
doaj   +1 more source

Effects of wheat root exudates on bacterial communities in the rhizosphere of watermelon

open access: yesPlant, Soil and Environment, 2021
In this study, we investigated the effects of wheat root exudates on soil bacterial communities in the watermelon rhizosphere using quantitative PCR and Illumina MiSeq sequencing.
Jibo Shi   +5 more
doaj   +1 more source

A5-miseq: an updated pipeline to assemble microbial genomes from Illumina MiSeq data [PDF]

open access: yesBioinformatics, 2014
Motivation: Open-source bacterial genome assembly remains inaccessible to many biologists because of its complexity. Few software solutions exist that are capable of automating all steps in the process of de novo genome assembly from Illumina data.
David Coil   +2 more
openaire   +4 more sources

Comparative analysis of targeted next-generation sequencing for Plasmodium falciparum drug resistance markers

open access: yesScientific Reports, 2022
Well-defined molecular resistance markers are available for a range of antimalarial drugs, and molecular surveillance is increasingly important for monitoring antimalarial drug resistance.
Chanon Kunasol   +6 more
doaj   +1 more source

Choosing a benchtop sequencing machine to characterise Helicobacter pylori genomes. [PDF]

open access: yesPLoS ONE, 2013
The fully annotated genome sequence of the European strain, 26695 was first published in 1997 and, in 1999, it was directly compared to the USA isolate J99, promoting two standard laboratory isolates for Helicobacter pylori (H. pylori) research. With the
Timothy T Perkins   +3 more
doaj   +1 more source

Illumina MiSeq sequencing disfavours a sequence motif in the GFP reporter gene. [PDF]

open access: yesSci Rep, 2016
AbstractGreen fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence.
Van den Hoecke S, Verhelst J, Saelens X.
europepmc   +5 more sources

Analysis, optimization and verification of Illumina-generated 16S rRNA gene amplicon surveys. [PDF]

open access: yesPLoS ONE, 2014
The exploration of microbial communities by sequencing 16S rRNA genes has expanded with low-cost, high-throughput sequencing instruments. Illumina-based 16S rRNA gene sequencing has recently gained popularity over 454 pyrosequencing due to its lower ...
Michael C Nelson   +4 more
doaj   +1 more source

Demultiplexed Illumina MiSeq ITS Sequences

open access: yes, 2022
Merged ITS sequences from paired-end Illumina MiSeq run ...
Clifton Bueno de Mesquita (10573952)
core   +1 more source

Illumina MiSeq raw sequence reads from arbuscular mycorrhizal fungi of grapevine and rye roots

open access: yes, 2021
Illumina MiSeq raw sequence reads of the partial LSU region of the rDNA gene obtained from grapevine and rye root mycorrhizal ...
Nogales, A
core   +2 more sources

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