A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq [PDF]
PeerJ, 2016 Background The bacterial 16S rRNA gene has historically been used in defining bacterial taxonomy and phylogeny. However, there are currently no high-throughput methods to sequence full-length 16S rRNA genes present in a sample with precision.
Catherine M. Burke, Aaron E. Darling
doaj +3 more sources
Saliva microbiome profiling by full-gene 16S rRNA Oxford Nanopore Technology versus Illumina MiSeq sequencing [PDF]
npj Biofilms and MicrobiomesMolecular characterization of the oral microbiome is a crucial first step in experiments which aim to understand the complex dynamics of the oral microbiome or the interplay with host health and disease.
Anders Esberg +3 more
doaj +2 more sources
Simultaneous SARS-CoV-2 Genome Sequencing of 384 Samples on an Illumina MiSeq Instrument through Protocol Optimization [PDF]
Genes, 2022 Nasserdine Papa Mze +2 more
exaly +2 more sources
The Effects of Freezing on Faecal Microbiota as Determined Using MiSeq Sequencing and Culture-Based Investigations [PDF]
, 2015 peer-reviewedBackground High-throughput sequencing has enabled detailed insights into complex microbial environments, including the human gut microbiota.
Deane, Jennifer +7 more
core +17 more sources
Comparison of Oxford Nanopore Technologies and Illumina MiSeq sequencing with mock communities and agricultural soil. [PDF]
Sci Rep, 2023 AbstractIllumina MiSeq is the current standard for characterizing microbial communities in soil. The newer alternative, Oxford Nanopore Technologies MinION sequencer, is quickly gaining popularity because of the low initial cost and longer sequence reads. However, the accuracy of MinION, per base, is much lower than MiSeq (95% versus 99.9%).
Stevens BM +3 more
europepmc +4 more sources
Identification of the Microbiota in Coconut Water, Kefir, Coconut Water Kefir and Coconut Water Kefir-Fermented Sourdough Using Culture-Dependent Techniques and Illumina–MiSeq Sequencing [PDF]
MicroorganismsMansi Limbad +2 more
exaly +2 more sources
MethodsX, 2023 During the last years, the application of next-generation sequencing (NGS) technologies to search for specific genetic markers has become a crucial method for the characterization of microbial communities.
Alexandra Coello-Camba +2 more
doaj +1 more source
Illumina DNA Prep (M) Tagmentation Library Preparation for use on an Illumina MiSeq Sequencer v1 [PDF]
, 2020 This procedure outlines the protocol for whole genome sequencing of bacterial organisms using the Illumina DNA Prep library preparation kit for sequencing on an Illumina MiSeq sequencer. This document applies to all laboratory personnel in the Division of Microbiology (DM) as well as laboratories in the GenomeTrakr Network.
Julie Haendiges +3 more
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Effects of wheat root exudates on bacterial communities in the rhizosphere of watermelon
Plant, Soil and Environment, 2021 In this study, we investigated the effects of wheat root exudates on soil bacterial communities in the watermelon rhizosphere using quantitative PCR and Illumina MiSeq sequencing.
Jibo Shi +5 more
doaj +1 more source
A5-miseq: an updated pipeline to assemble microbial genomes from Illumina MiSeq data [PDF]
Bioinformatics, 2014 Motivation: Open-source bacterial genome assembly remains inaccessible to many biologists because of its complexity. Few software solutions exist that are capable of automating all steps in the process of de novo genome assembly from Illumina data.
David Coil +2 more
openaire +3 more sources