Simultaneous SARS-CoV-2 Genome Sequencing of 384 Samples on an Illumina MiSeq Instrument through Protocol Optimization [PDF]
Genes, 2022 In the present study, we propose a high-throughput sequencing protocol using aNextera XT Library DNA kit on an Illumina MiSeq instrument. We made major modifications to this library preparation in order to multiplex 384 samples in a single Illumina flow ...
Philippe Colson +2 more
exaly +3 more sources
A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq [PDF]
PeerJ, 2016 Background The bacterial 16S rRNA gene has historically been used in defining bacterial taxonomy and phylogeny. However, there are currently no high-throughput methods to sequence full-length 16S rRNA genes present in a sample with precision.
Catherine M. Burke, Aaron E. Darling
doaj +5 more sources
Illumina MiSeq Analysis and Comparison of Freshwater Microalgal Communities on Ulleungdo and Dokdo Islands [PDF]
Polish Journal of Microbiology, 2019 Abstract Ulleungdo and Dokdo are volcanic islands with an oceanic climate located off the eastern coast of South Korea. In the present study, we used barcoded Illumina MiSeq to analyze eukaryotic microalgal genera collected from Seonginbong, the highest peak on Ulleungdo, and from groundwater sites on Dongdo and Seodo Islands, which ...
HYUN-SIK YUN +2 more
doaj +5 more sources
Insights into bacterioplankton community structure from Sundarbans mangrove ecoregion using Sanger and Illumina MiSeq sequencing approaches: A comparative analysis [PDF]
Genomics Data, 2017 Next generation sequencing using platforms such as Illumina MiSeq provides a deeper insight into the structure and function of bacterioplankton communities in coastal ecosystems compared to traditional molecular techniques such as clone library approach ...
Anwesha Ghosh, Punyasloke Bhadury
doaj +2 more sources
PLoS ONE, 2017 Metagenetics is a rapid and taxonomically comprehensive method for revealing community structures within environmental samples, based on large amounts of sequence data produced by high-throughput sequencers.
Junya Hirai +2 more
doaj +2 more sources
Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform [PDF]
Nucleic Acids Research, 2015 With read lengths of currently up to 2 × 300 bp, high throughput and low sequencing costs Illumina's MiSeq is becoming one of the most utilized sequencing platforms worldwide. The platform is manageable and affordable even for smaller labs.
Ijaz, Umer Z. +7 more
core +6 more sources
Saliva microbiome profiling by full-gene 16S rRNA Oxford Nanopore Technology versus Illumina MiSeq sequencing [PDF]
npj Biofilms and MicrobiomesMolecular characterization of the oral microbiome is a crucial first step in experiments which aim to understand the complex dynamics of the oral microbiome or the interplay with host health and disease.
Anders Esberg +3 more
doaj +2 more sources
, 2020 Metabarcoding of fungi and bacteria is rapidly evolving as a popular tool to assess the composition of microbial communities associated with decomposing plant litter.
Christian Wurzbacher +5 more
core +3 more sources
Illumina MiSeq and iSeq platforms yield comparable results for viral genomic sequencing. [PDF]
J Virol MethodsIllumina MiSeq and iSeq are widely used short-read next-generation sequencing (NGS) platforms. The MiSeq instrument is specialized for mid-range throughput, while the iSeq instrument has lower throughput but is less expensive and simpler to operate. Several studies have compared Illumina platforms for sequencing of a variety of specimen types, but very
Wagner DD +3 more
europepmc +3 more sources
Illumina DNA Prep (M) Tagmentation Library Preparation for use on an Illumina MiSeq Sequencer v1 [PDF]
, 2020 This procedure outlines the protocol for whole genome sequencing of bacterial organisms using the Illumina DNA Prep library preparation kit for sequencing on an Illumina MiSeq sequencer. This document applies to all laboratory personnel in the Division of Microbiology (DM) as well as laboratories in the GenomeTrakr Network.
Julie Haendiges +3 more
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