Results 31 to 40 of about 141,743 (278)
Scientific Reports, 2022 Well-defined molecular resistance markers are available for a range of antimalarial drugs, and molecular surveillance is increasingly important for monitoring antimalarial drug resistance.
Chanon Kunasol +6 more
doaj +1 more source
Choosing a benchtop sequencing machine to characterise Helicobacter pylori genomes. [PDF]
PLoS ONE, 2013 The fully annotated genome sequence of the European strain, 26695 was first published in 1997 and, in 1999, it was directly compared to the USA isolate J99, promoting two standard laboratory isolates for Helicobacter pylori (H. pylori) research. With the
Timothy T Perkins +3 more
doaj +1 more source
Illumina MiSeq sequencing disfavours a sequence motif in the GFP reporter gene. [PDF]
Sci Rep, 2016 AbstractGreen fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence.
Van den Hoecke S, Verhelst J, Saelens X.
europepmc +5 more sources
Fluorescent amplification for next generation sequencing (FA-NGS) library preparation. [PDF]
, 2020 BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive ...
Chiniquy, Jennifer +3 more
core +1 more source
Analysis, optimization and verification of Illumina-generated 16S rRNA gene amplicon surveys. [PDF]
PLoS ONE, 2014 The exploration of microbial communities by sequencing 16S rRNA genes has expanded with low-cost, high-throughput sequencing instruments. Illumina-based 16S rRNA gene sequencing has recently gained popularity over 454 pyrosequencing due to its lower ...
Michael C Nelson +4 more
doaj +1 more source
Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform [PDF]
Nucleic Acids Research, 2015 With read lengths of currently up to 2 × 300 bp, high throughput and low sequencing costs Illumina's MiSeq is becoming one of the most utilized sequencing platforms worldwide. The platform is manageable and affordable even for smaller labs. This enables quick turnaround on a broad range of applications such as targeted gene sequencing, metagenomics ...
Schirmer, Melanie +5 more
openaire +4 more sources
Genomics Data, 2017 Next generation sequencing using platforms such as Illumina MiSeq provides a deeper insight into the structure and function of bacterioplankton communities in coastal ecosystems compared to traditional molecular techniques such as clone library approach ...
Anwesha Ghosh, Punyasloke Bhadury
doaj +1 more source
QQ-SNV: single nucleotide variant detection at low frequency by comparing the quality quantiles [PDF]
, 2015 Background: Next generation sequencing enables studying heterogeneous populations of viral infections. When the sequencing is done at high coverage depth ("deep sequencing"), low frequency variants can be detected.
Aerssens, Jeroen +7 more
core +4 more sources
PLoS ONE, 2017 Metagenetics is a rapid and taxonomically comprehensive method for revealing community structures within environmental samples, based on large amounts of sequence data produced by high-throughput sequencers.
Junya Hirai +2 more
doaj +1 more source
16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform [PDF]
, 2016 peer-reviewedBackground Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the
Claesson, Marcus J. +4 more
core +2 more sources