Results 31 to 40 of about 156,271 (269)

Illuminating Choices for Library Prep: A Comparison of Library Preparation Methods for Whole Genome Sequencing of Cryptococcus neoformans Using Illumina HiSeq. [PDF]

, 2014
The industry of next-generation sequencing is constantly evolving, with novel library preparation methods and new sequencing machines being released by the major sequencing technology companies annually.
Mathew A Beale, Fisher, Matthew C, Rhodes Johanna, Matthew C Fisher, Rhodes, Johanna, Beale Mathew A., Fisher, MC, Beale, Mathew A, Fisher, Matthew C., Beale, Mathew A., Fisher Matthew C., Beale, MA, Rhodes, J, Johanna Rhodes, Rhodes, Johanna; id_orcid   +14 more
core   +1 more source

Bacterial diversity and community in Qula from the Qinghai–Tibetan Plateau in China [PDF]

PeerJ, 2018
Qula is a cheese-like product usually prepared with unpasteurized yak milk under open conditions, with both endogenous and exogenous microorganisms involved in the fermentation process.
Yan Zhu, Yingying Cao, Min Yang, Pengchen Wen, Lei Cao, Jiang Ma, Zhongmin Zhang, Weibing Zhang   +7 more
doaj   +2 more sources

Illumina iSeq 100 and MiSeq exhibit similar performance in freshwater fish environmental DNA metabarcoding

Scientific Reports, 2021
Environmental DNA (eDNA) analysis is a method of detecting DNA from environmental samples and is used as a biomonitoring tool. In recent studies, Illumina MiSeq has been the most extensively used tool for eDNA metabarcoding.
Ryohei Nakao, Ryutei Inui, Yoshihisa Akamatsu, Masuji Goto, Hideyuki Doi, Shunsuke Matsuoka   +5 more
doaj   +1 more source

List of primers used for PCR analysis or environmental DNA metabarcoding sequencing by Illumina MiSeq platform.

, 2022
List of primers used for PCR analysis or environmental DNA metabarcoding sequencing by Illumina MiSeq platform.
Ryosuke Kimura (273945), Tetsu Yamashiro (3743845), Tadashi Kajita (587646), Idam Hermawan (9276623), Yukuto Sato (68176), Claudia Toma (78079), Hideyuki Imai (191913), Hiroto Nagai (12328652), Tetsuya Kakita (4922584), Sho Okano (4922575)   +9 more
core   +1 more source

Sample preparation for Illumina MiSeq Dual Index metabarcoding v1 [PDF]

, 2020
The protocol shows amplicon sequencing samples preoared by Illumina Dual Index strategy, and sequened with Illumina MiSeq (RRID:SCR_016379) for bacteria and microeukaryotes.
Xiaohuan Sun, Yuehua Hu, Zewei Song
openaire   +1 more source

Metagenomic investigation of faecal microbiota in sheep and goats of the same ages

Journal of Taibah University for Science, 2021
Despite the significant influence of the microbial communities that inhabit the ruminant’s gastrointestinal tract on their health and production, the study of the composition and variations in sheep and goats gut microbiome is limited.
I. I. Shabana, N. N. Albakri, N. A. Bouqellah   +2 more
doaj   +1 more source

cpn60 metagenomic amplicon library preparation for the Illumina Miseq platform

, 2021
Abstract This protocol can be applied to determine the composition of a microbial community. The cpn60 gene (also known as groEL, hsp60) is present in almost all bacteria and a 552-558 bp region of the gene has been established as a barcode for species level identification of bacteria. The primer cocktail used in this
Champika Fernando, Janet E. Hill
openaire   +1 more source

Phasing amplicon sequencing on Illumina Miseq for robust environmental microbial community analysis [PDF]

BMC Microbiology, 2015
Although high-throughput sequencing, such as Illumina-based technologies (e.g. MiSeq), has revolutionized microbial ecology, adaptation of amplicon sequencing for environmental microbial community analysis is challenging due to the problem of low base diversity.A new phasing amplicon sequencing approach (PAS) was developed by shifting sequencing phases
Wu, Liyou, Wen, Chongqing, Qin, Yujia, Yin, Huaqun, Tu, Qichao, Van Nostrand, Joy D., Yuan, Tong, Yuan, Menting, Deng, Ye, Zhou, Jizhong   +9 more
openaire   +2 more sources

Species identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]

, 2013
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Niranjan Nagarajan, Eliza Xin Pei Ho, Andreas Wilm (7951), Hibberd, Martin Lloyd, Louie Low (400408), Nagarajan, Niranjan, Eliza Xin Pei Ho (400407), Ong, Swee Hoe, Christophe Lay (400406), Martin Lloyd Hibberd (259920), Low, Louie, Kukkillaya, Vinutha Uppoor, Andreas Wilm, Martin Lloyd Hibberd, Lay, Christophe, Niranjan Nagarajan (131213), Wilm, Andreas, Ho, Eliza Xin Pei, Vinutha Uppoor Kukkillaya (400405), Swee Hoe Ong, Louie Low, Swee Hoe Ong (400404), Vinutha Uppoor Kukkillaya, Christophe Lay   +23 more
core   +1 more source

A shared mucosal gut microbiota signature in primary sclerosing cholangitis before and after liver transplantation

Hepatology, EarlyView., 2022
In this study of the gut mucosal microbiota in primary sclerosing cholangitis (PSC), we found consistent microbiota features associated with PSC and recurrent PSC, PSC with inflammatory bowel disease and a persistent gut dysbiosis after liver transplantation Abstract Background and Aims Several characteristic features of the fecal microbiota have been ...
Mikal Jacob Hole, Kristin Kaasen Jørgensen, Kristian Holm, Peder R. Braadland, Malin Holm Meyer‐Myklestad, Asle Wilhelm Medhus, Dag Henrik Reikvam, Alexandra Götz, Krzysztof Grzyb, Kirsten Muri Boberg, Tom Hemming Karlsen, Martin Kummen, Johannes R. Hov   +12 more
wiley   +1 more source