Results 111 to 120 of about 156,271 (269)

Preparation of Amplicon Libraries for Metabarcoding of Marine Eukaryotes Using Illumina MiSeq: The Adapter Ligation Method

, 2016
Amplicon-based studies of marine microscopic eukaryotes, also referred to as metabarcoding studies, can be performed to analyze patterns of biodiversity or predator–prey interactions targeting the mitochondrial cytochrome oxidase 1 (CO1) or the small ...
Leray, Matthieu, Haenel, Quiterie,, Quiterie Haenel, Matthieu Leray, Sarah J. Bourlat, Bourlat, Sarah,   +5 more
core   +1 more source

Scale‐dependent assembly shapes taxon‐dependent responses of soil microbes to island isolation and area

Ecography, EarlyView.
The theory of island biogeography (TIB) remains contentious for microbes, as their immense dispersal potential and microscopic size may decouple diversity from classic isolation/area effects. We explicitly tested TIB scaling rules for soil bacteria and fungi across 20 oceanic islands using a rigorous multi‐scale framework that integrated high ...
Lan Liu, Zuzana Münzbergová, Lele Jiang, Long Chen, Shurong Zhou   +4 more
wiley   +1 more source

Comparative analyses of the results of HLA genes typing by NGS method using different platforms

Медицинская иммунология
Next generation sequencing (NGS) allows high-resolution and allelic HLA-typing. The most common platform is MiSeq (Illumina, USA). Currently, the maintenance of this device is difficult, which has necessitated the search for analogues that are not ...
I. E. Pavlova, E. V. Kuzmich, E. G. Khamaganova, D. V. Rudik, E. P. Kuzminova, A. R. Abdrakhimova   +5 more
doaj   +1 more source

A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers

BMC Genomics, 2012
Background Next generation sequencing (NGS) technology has revolutionized genomic and genetic research. The pace of change in this area is rapid with three major new sequencing platforms having been released in 2011: Ion Torrent’s PGM, Pacific ...
Quail Michael A, Smith Miriam, Coupland Paul, Otto Thomas D, Harris Simon R, Connor Thomas R, Bertoni Anna, Swerdlow Harold P, Gu Yong   +8 more
doaj   +1 more source

Improved pipeline for reducing erroneous identification by 16S rRNA sequences using the Illumina MiSeq platform

, 2015
The cost of DNA sequencing has decreased due to advancements in Next Generation Sequencing. The number of sequences obtained from the lllumina platform is large, use of this platform can reduce costs more than the 454 pyrosequencer. However, the Illumina
Kim, Bong-Soo, Jeon, Yoon-Seong, Lim, Jeongmin, Chun, Jongsik, Park, Sang-Cheol   +4 more
core   +1 more source

Candida auris sequencing by Illumina miSeq using Illumina DNA Prep v1

Candida auris sequencing is becoming more important for public health and surveillance. This protocol is designed to guide individuals experienced in sequencing in setting up a SOP for sequencing C. auris. This protocol is designed for Illumina short-read sequencing.
openaire   +1 more source

Ketogenic diet fails to mitigate seizures and neuroinflammatory responses in a mouse model of virus‐induced epilepsy

Epilepsia, EarlyView.
Abstract Objective The ketogenic diet (KD) is a high‐fat, low‐carbohydrate intervention widely used to treat drug‐resistant epilepsy, thought to reduce seizures through a combination of metabolic, neuronal, and microbiota‐dependent mechanisms. Additionally, recent studies suggest that the anticonvulsant effects of KD require the gut microbiota, with ...
Casey H. Meili, Kaitlyn Allen, Daniel J. Doty, Sofia Del Fiol, Ana Beatriz DePaula‐Silva   +4 more
wiley   +1 more source

Alterations of the Gut Microbiome Associated With the Treatment of Hyperuricaemia in Male Rats

Frontiers in Microbiology, 2018
Hyperuricaemia is an important risk factor for many diseases including gout, hypertension, and type II diabetes. The gut microbiota is associated with hyperuricaemia and has also been demonstrated to play significant roles in the effects of drug therapy.
Yiran Yu, Qiuping Liu, Haichang Li, Chengping Wen, Zhixing He   +4 more
doaj   +1 more source

Exploring medical food supplementation as an adjunct strategy for microbiota restoration and seizure reduction in CDKL5 deficiency disorder

Epilepsia Open, EarlyView.
Abstract Objectives CDKL5 deficiency disorder (CDD) is a developmental and epileptic encephalopathy characterized by early‐onset, treatment‐resistant seizures and severe neurodevelopmental impairment. Increasing evidence suggests that alterations in the gut–brain axis may contribute to disease pathophysiology, raising interest in microbiota‐targeted ...
Elisa Borghi, Matteo Domenico Marsiglia, Francesca Triva, Emerenziana Ottaviano, Ilaria Viganò, Miriam Nella Savini, Valentino Costabile, Aglaia Vignoli   +7 more
wiley   +1 more source

Exploring the microbial community (microflora) associated with ovine Haemonchus contortus (macroflora) field strains

Scientific Reports, 2017
High-throughput sequencing technology has shown tremendous promise for microbial community composition and diversity. Illumina MiSeq platform was exploited to study the microbial community associated with the different stages of the life-cycle of ovine ...
Saeed El-Ashram, Xun Suo
doaj   +1 more source