Results 91 to 100 of about 102,930 (272)

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

Whole Genome Sequencing of Influenza A and B Viruses With the MinION Sequencer in the Clinical Setting: A Pilot Study

Frontiers in Microbiology, 2018
Introduction: Whole genome sequencing (WGS) of influenza viruses is important for preparing vaccines and coping with newly emerging viruses. However, WGS is difficult to perform using conventional next-generation sequencers in developing countries, where
Kazuo Imai, Kazuo Imai, Kazuo Imai, Kaku Tamura, Tomomi Tanigaki, Mari Takizawa, Eiko Nakayama, Takahiko Taniguchi, Misako Okamoto, Yasumasa Nishiyama, Norihito Tarumoto, Norihito Tarumoto, Kotaro Mitsutake, Kotaro Mitsutake, Takashi Murakami, Takashi Murakami, Shigefumi Maesaki, Shigefumi Maesaki, Takuya Maeda, Takuya Maeda   +19 more
doaj   +1 more source

Illumina MiSeq and iSeq platforms yield comparable results for viral genomic sequencing

Journal of Virological Methods
Illumina MiSeq and iSeq are widely used short-read next-generation sequencing (NGS) platforms. The MiSeq instrument is specialized for mid-range throughput, while the iSeq instrument has lower throughput but is less expensive and simpler to operate. Several studies have compared Illumina platforms for sequencing of a variety of specimen types, but very
Darlene D. Wagner, Grace Nabakooza, Nehalraza Momin, Rachel L. Marine   +3 more
openaire   +2 more sources

Clinical metagenomic sequencing - CSF RNA and DNA Illumina MiSeq v1

, 2022
This protocol was developed to evaluate unbiased cerebrospinal fluid metagenomics at a referral centre in South Africa. Commercial kit protocols are generally used unmodified and limited use of in-house developed methods or reagents is made. This maintains the simplicity and accessibility of the protocol which can serve as a base for evaluation of ...
Gert Marais, Ziyaad Valley-Omar, Kathleen Bateman, Kate McMullen, Dieter van der Westhuizen, Moepeng Maseko, Suzaan Marais, Diana Hardie, Adrian Brink   +8 more
openaire   +1 more source

Microbial community of broadleaved forests characterized by Illumina MiSeq sequencing

, 2015
As two major forest types in the subtropics, broadleaved evergreen and broadleaved deciduous forests have long interested ecologists. Here, we used Illumina MiSeq sequencing targeting 16S rRNA gene to analyze microbial communities in broadleaved ...
Yuguang Zhang (434842), Junjun Ding (249893), Yunfeng Yang (47638)   +2 more
core   +1 more source

An amplicon-based approach for sequencing of SARS-CoV-2 using COVIDSeq assay on Illumina MiSeq sequencing platforms

, 2021
Sequencing genomes of SARS-CoV-2 is crucial for understanding the genetic epidemiology of COVID-19pandemic. It is also critical for understanding the evolution of the virus and also for the rapid development of the diagnostic tools. The present protocol
Bani Jolly, Mohamed Imran, Sridhar Sivasubbu, Abhinav Jain, Vigneshwar Senthivel, Mohit kumar divakar, Rahul C. Bhoyar, Vinod Scaria   +7 more
core   +1 more source

Dietary Baicalin Supplementation Can Enhance the Growth Performance of Weaned Piglets and Maintain the Intestinal Barrier Integrity

Animal Research and One Health, EarlyView.
This research innovatively illustrates baicalin’s multifaceted mechanisms in enhancing piglets’ intestinal health: modulating bile acid metabolism via probiotics, reinforcing tight junction proteins (ZO‐1/claudin), suppressing TLR4/NF‐κB‐mediated inflammation, whereas promoting growth and reducing diarrhea.
Yuhui Gao, Jie Liu, Rui Yang, Xizhen Fan, Cheng Liang, Xin'e Shi, Jianjun Jin   +6 more
wiley   +1 more source

Data from: Massively parallel multiplex DNA sequencing for specimen identification using an Illumina MiSeq platform

, 2015
Genetic information is a valuable component of biosystematics, especially specimen identification through the use of species-specific DNA barcodes. Although many genomics applications have shifted to High-Throughput Sequencing (HTS) or Next-Generation ...
Porter, Teresita M., Janzen, Daniel H., Hajibabaei, Mehrdad, Golding, G. Brian, Gibson, Joel F., Shokralla, Shadi, Dobosz, Rafal, Hallwachs, Winnie   +7 more
core   +1 more source

Effects of Lycium barbarum Residue Substituting Dietary Maize on Fecal Microbiota and Growth Performance of Crossbred Simmental Cattle

Animal Research and One Health, EarlyView.
The bioactive substances we extracted from Lycium barbarum residue (LBR) have a typical polysaccharide structure. When 1.80% LBR was used to replace maize in the diet, the average daily gain of rossbred Simmental was significantly increased, and anti‐inflammatory ability was improved. In addition, LBR improved the fecal microbial composition, increased
Kun Cai, Mingtan Li, Lihang He, Ning Ma, Haoyu Liu, Chi Xu, Xianhua Wu, Zeguang Xing, Qian Xing, Yuankai Qu, Xi Ma   +10 more
wiley   +1 more source

Preparing multiplexed WGS/MetaG libraries with the Illumina DNA Prep kit for the Illumina NextSeq or MiSeq v1

, 2022
The preparation of (meta)genomic libraries using the Illumina Nextera Flex (now "DNA Prep") kit with CD/UDI indices at the IMR. Based upon Illumina protocol 10000000254 16 v10 (Aug.2021).
openaire   +1 more source