Results 71 to 80 of about 156,271 (269)

A quantitative and qualitative comparison of illumina MiSeq and 454 amplicon sequencing for genotyping the highly polymorphic major histocompatibility complex (MHC) in a non-model species

open access: yesBMC Research Notes, 2017
Background High-throughput sequencing enables high-resolution genotyping of extremely duplicated genes. 454 amplicon sequencing (454) has become the standard technique for genotyping the major histocompatibility complex (MHC) genes in non-model organisms.
Haslina Razali   +4 more
doaj   +1 more source

Whole-Genome Sequencing of Human Enteroviruses from Clinical Samples by Nanopore Direct RNA Sequencing

open access: yesViruses, 2020
Enteroviruses are small RNA viruses that affect millions of people each year by causing an important burden of disease with a broad spectrum of symptoms.
Carole Grädel   +7 more
doaj   +1 more source

DGGE and Illumina Miseq based diversity of microeukaryotic communities from Xiamen offshore sea.

open access: yes, 2015
DGGE band number (A) and Illumina Miseq based OTU number (B), Shannon–Wiener index based on DGGE profiles (C) and Miseq sequencing data (D). (N, northern sites; E, eastern sites; S, southern sites; W, western sites).
Lingyu Yu (746506)   +3 more
core   +1 more source

16S Sequencing on Illumina MiSeq Platform v1

open access: yes
The 16S protocol detailed here is designed to amplify bacteria using V4-V5 hyper-variable regions of the 16S rRNA gene on the Illumina MiSeq DNA sequencer.
Angela Schmoldt, Melissa Schussman
openaire   +1 more source

Microbial Community of Tannery Wastewater Involved in Nitrification Revealed by Illumina MiSeq Sequencing

open access: yesJournal of Microbiology and Biotechnology, 2018
The aim of this study was to investigate the microbial community of three tannery wastewater treatment plants (WWTPs) involved in nitrification by Illumina MiSeq sequencing. The results showed that highly diverse communities were present in tannery wastewater.
Xiaojian, Ma   +4 more
openaire   +3 more sources

Preparing multiplexed 16S/18S/ITS amplicons for the Illumina MiSeq v1

open access: yes, 2022
The following detailed protocol is for the generation of paired-end sequencing reads of 16S/18S/ITS PCR amplicons with dual barcodes (i.e.: “indices”) on the Illumina MiSeq machine of length ≈450 bp (ie: 300+300 bp sequencing with ~150 bp overlap) using v3 600 cycle chemistry. It assumes an input of 380 individual samples (+4 controls = 384) in four 96-
André M Comeau, Alessi Kwawukume
openaire   +1 more source

Placing very long branch taxa in the plant tree of life: A case study with Dioscoreales mycoheterotrophs

open access: yesAmerican Journal of Botany, EarlyView.
Abstract Premise The inclusion of heterotrophic plants in broader systematic studies is challenging due to substantial morphological modification, gene loss, and accelerated substitution rates following photosynthesis loss. In particular, inclusion of very long branch (VLB) taxa with highly elevated rates may lead to phylogenomic misinference.
Marybel Soto Gomez   +7 more
wiley   +1 more source

Dual Aberrant Splicing Caused by an Apparently Missense CHD7 Variant, c.5273A>G (p.Asp1758Gly), in CHARGE Syndrome

open access: yesAmerican Journal of Medical Genetics Part A, EarlyView.
ABSTRACT CHARGE syndrome is a rare congenital disorder primarily attributed to heterozygous pathogenic variants of the CHD7 gene. Most pathogenic CHD7 variants are loss‐of‐function (LoF) variants, whereas the interpretation of missense variants remains challenging in the absence of functional evidence for their pathogenicity.
Takashi Okuno   +8 more
wiley   +1 more source

Metagenomic profiling dataset of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O'Kiep)

open access: yesData in Brief, 2019
The metagenomic data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O'Kiep, Namaqualand, South Africa.
Innocentia G. Erdogan   +4 more
doaj   +1 more source

An Assessment of the Bacterial Diversity found in Dental Unit Waterlines using the Illumina MiSeq

open access: yesBiocontrol Science, 2019
Water from the waterlines of dental units is often contaminated with bacteria but there have been few studies accurately assessing the diversity of these bacterial populations. The aim of our study was to assess the bacterial diversity present in water collected from dental unit waterlines using the Illumina MiSeq. Water was collected from two separate
YOON, HYE YOUNG, LEE, SI YOUNG
openaire   +3 more sources

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