Results 81 to 90 of about 156,271 (269)
Clinical metagenomic sequencing - CSF RNA and DNA Illumina MiSeq v1
, 2022 This protocol was developed to evaluate unbiased cerebrospinal fluid metagenomics at a referral centre in South Africa. Commercial kit protocols are generally used unmodified and limited use of in-house developed methods or reagents is made. This maintains the simplicity and accessibility of the protocol which can serve as a base for evaluation of ...
Gert Marais +8 more
openaire +1 more source
A rhesus macaque model of α‐dystroglycanopathy caused by a POMT1 splice altering variant
Animal Models and Experimental Medicine, EarlyView.Rhesus macaques homozygous for a naturally occurring spice altering variant in the POMT1 gene develop lissencephaly, microphthalmia, and muscular contracture. The presentation models severe presentation of the human Walker‐Warburg syndrome. Abstract Background Biallelic mutations in genes associated with α‐dystroglycan glycosylation manifest in a ...
Anya Nordlund +7 more
wiley +1 more source
Illumina MiSeq data ITS2 Vascular plants
, 2017 Contains 12 fastq files corresponding to R1 and R2 data from 6 Illumina MiSeq 250 bp PE sequencing libraries. 130 soil samples from Denmark were amplified with tagged primers (S2F and ITS4) and build into TruSeq PCR free libraries.
Kjøller, Rasmus +15 more
core +1 more source
, 2022 The preparation of (meta)genomic libraries using the Illumina Nextera Flex (now "DNA Prep") kit with CD/UDI indices at the IMR. Based upon Illumina protocol 10000000254 16 v10 (Aug.2021).
openaire +1 more source
Patient‐Derived Fibroblasts as a Clinically Relevant Model of Kearns–Sayre Syndrome
Annals of Neurology, EarlyView.Objective Kearns–Sayre syndrome (KSS) is characterized by single large‐scale mitochondrial DNA deletions and by severe early‐onset clinical manifestations with neurological involvement. Reliable disease models, as well as validated biomarkers or effective treatments, are lacking.
Laura Valls‐Roca +24 more
wiley +1 more source
Comparison of the performance of MiSeq and NovaSeq in oral microbiome study
Journal of Oral MicrobiologyObjective Next generation sequencing is commonly used to characterize the microbiome structure. MiSeq is most commonly used to analyze the microbiome due to its relatively long read length. Illumina also introduced the 250 × 2 chip for NovaSeq.
Hyejung Han +4 more
doaj +1 more source
Animal Research and One Health, EarlyView.This study aimed to investigate the effects of cellulase and Lactobacillus plantarum on the silage quality, in situ digestibility, and microbial communities of apple pomace silage. The combined supplementation of cellulase and Lactobacillus plantarum in apple pomace silage improved fermentation quality and enhanced rumen utilization efficiency.
Zhuangzhuang Liu +4 more
wiley +1 more source
BMC Medical GenomicsBackground Pharmacogenomics of hydroxyurea is an important aspect in the management of sickle cell disease (SCD), especially in the era of genomic medicine.
Siana Nkya +17 more
doaj +1 more source
, 2019 Nucleotide sequence accession numbers, lengths of genes obtained from Illumina MiSeq sequencing and closest strain based on phylogeny.
Sabina Kwofie (6843443) +9 more
core +1 more source
Animal Research and One Health, EarlyView.We conducted a longitudinal trial across nursery, growing, and finishing phases, showing that phytochemical supplementation as a potential antibiotic alternative reduced potential pathogens and promoted beneficial Lactobacillus amylovorus in the nursery phase, and enriched amino acid and carbohydrate metabolism pathways (prediction) during finishing ...
Ziyu Liu +11 more
wiley +1 more source