A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq [PDF]
PeerJ, 2016 Background The bacterial 16S rRNA gene has historically been used in defining bacterial taxonomy and phylogeny. However, there are currently no high-throughput methods to sequence full-length 16S rRNA genes present in a sample with precision.
Catherine M. Burke, Aaron E. Darling
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Characterization of Microsatellite Markers for Pinedrops, Pterospora andromedea (Ericaceae), from Illumina MiSeq sequencing [PDF]
Applications in Plant Sciences, 2014 Premise of the study: Pterospora andromedea (Ericaceae) is a mycoheterotrophic plant endemic to North America with a disjunct distribution. Eastern populations are in decline compared to western populations.
Lisa C. Grubisha +4 more
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A bumpy ride on the diagnostic bench of massive parallel sequencing, the case of the mitochondrial genome [PDF]
, 2014 The advent of massive parallel sequencing (MPS) has revolutionized the field of human molecular genetics, including the diagnostic study of mitochondrial (mt) DNA dysfunction.
Caljon, Ben +11 more
core +21 more sources
Illumina MiSeq Analysis and Comparison of Freshwater Microalgal Communities on Ulleungdo and Dokdo Islands [PDF]
Polish Journal of Microbiology, 2019 HYUN-SIK YUN +2 more
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MethodsX, 2023 During the last years, the application of next-generation sequencing (NGS) technologies to search for specific genetic markers has become a crucial method for the characterization of microbial communities.
Alexandra Coello-Camba +2 more
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A5-miseq: an updated pipeline to assemble microbial genomes from Illumina MiSeq data [PDF]
Bioinformatics, 2014 Motivation: Open-source bacterial genome assembly remains inaccessible to many biologists because of its complexity. Few software solutions exist that are capable of automating all steps in the process of de novo genome assembly from Illumina data.
Coil, David +2 more
openaire +3 more sources
Effects of wheat root exudates on bacterial communities in the rhizosphere of watermelon
Plant, Soil and Environment, 2021 In this study, we investigated the effects of wheat root exudates on soil bacterial communities in the watermelon rhizosphere using quantitative PCR and Illumina MiSeq sequencing.
Jibo Shi +5 more
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Scientific Reports, 2022 Well-defined molecular resistance markers are available for a range of antimalarial drugs, and molecular surveillance is increasingly important for monitoring antimalarial drug resistance.
Chanon Kunasol +6 more
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Fluorescent amplification for next generation sequencing (FA-NGS) library preparation. [PDF]
, 2020 BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive ...
Chiniquy, Jennifer +3 more
core +1 more source
Reproducibility of methylated CpG typing with the Illumina MiSeq [PDF]
Forensic Science International: Genetics Supplement Series, 2017 Abstract DNA methylation patterns may be used for identification of body fluids and for age estimation of human individuals. We evaluated some of the challenges and pitfalls of studying methylated CpG sites. We compared the methylated CpG analysis of two different methods 1) massively parallel sequencing (MPS) using the Illumina MiSeq and 2) the iPLEX
Kampmann, Marie-Louise +4 more
openaire +2 more sources