Differences in the gut microbiota of dogs (Canis lupus familiaris) fed a natural diet or a commercial feed revealed by the Illumina MiSeq platform. [PDF]
BackgroundRecent advances in next-generation sequencing technologies have enabled comprehensive analysis of the gut microbiota, which is closely linked to the health of the host.
Kim J, An JU, Kim W, Lee S, Cho S.
europepmc +2 more sources
Bacterial and eukaryotic microbial communities in urban water systems profiled via Illumina MiSeq platform. [PDF]
Ting ASY +5 more
europepmc +2 more sources
A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq [PDF]
Background The bacterial 16S rRNA gene has historically been used in defining bacterial taxonomy and phylogeny. However, there are currently no high-throughput methods to sequence full-length 16S rRNA genes present in a sample with precision.
Catherine M. Burke, Aaron E. Darling
doaj +3 more sources
Changes in the Soil Microbiome in Eggplant Monoculture Revealed by High-Throughput Illumina MiSeq Sequencing as Influenced by Raw Garlic Stalk Amendment. [PDF]
The incorporation of plant residues into soil can be considered a keystone sustainability factor in improving soil structure function. However, the effects of plant residue addition on the soil microbial communities involved in biochemical cycles and ...
Ghani MI +7 more
europepmc +2 more sources
Comparing dental plaque microbiome diversity of extrinsic black stain in the primary dentition using Illumina MiSeq sequencing technique. [PDF]
Extrinsic black stain (EBS) is characterized by discrete dark dots or lines on the tooth surface. The relationship between EBS and oral microbiota in children remains elusive.
Chen L, Zhang Q, Wang Y, Zhang K, Zou J.
europepmc +2 more sources
High-Throughput Illumina MiSeq Amplicon Sequencing of Yeast Communities Associated With Indigenous Dairy Products From Republics of Benin and Niger. [PDF]
Traditional Wagashi cheese and fermented cow milk are among the most popular dairy products appreciated by people from Benin, Niger, and the neighboring region. These products are the main source of protein in the diet of the low-income population in the
Sessou P +5 more
europepmc +2 more sources
Illumina MiSeq sequencing disfavours a sequence motif in the GFP reporter gene. [PDF]
AbstractGreen fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence.
Van den Hoecke S, Verhelst J, Saelens X.
europepmc +5 more sources
Phasing amplicon sequencing on Illumina Miseq for robust environmental microbial community analysis. [PDF]
Although high-throughput sequencing, such as Illumina-based technologies (e.g. MiSeq), has revolutionized microbial ecology, adaptation of amplicon sequencing for environmental microbial community analysis is challenging due to the problem of low base diversity.A new phasing amplicon sequencing approach (PAS) was developed by shifting sequencing phases
Wu L +9 more
europepmc +4 more sources
An optimized, amplicon-based approach for sequencing of SARS-CoV-2 from patient samples using COVIDSeq assay on Illumina MiSeq sequencing platforms. [PDF]
Bhoyar RC +7 more
europepmc +2 more sources
Characterization of Microsatellite Markers for Pinedrops, Pterospora andromedea (Ericaceae), from Illumina MiSeq sequencing [PDF]
Premise of the study: Pterospora andromedea (Ericaceae) is a mycoheterotrophic plant endemic to North America with a disjunct distribution. Eastern populations are in decline compared to western populations.
Lisa C. Grubisha +4 more
doaj +3 more sources

