Results 51 to 60 of about 139,474 (279)
Evaluating the capacity of human gut microorganisms to colonize the zebrafish larvae (Danio rerio) [PDF]
, 2018 Indexación: Scopus.In this study we evaluated if zebrafish larvae can be colonized by human gut microorganisms. We tested two strategies: (1) through transplantation of a human fecal microbiota and (2) by successively transplanting aerotolerant anaerobic
Angelica Reyes-Jara +14 more
core +2 more sources
Illumina MiSeq Dual-barcoded Two-step PCR Amplicon Sequencing Protocol v1
, 2020 Background Information High throughput amplicon sequencing is an extremely sensitive technique. The lab environment is full of airborne bacterial and fungal cells as well as bacterial and fungal genomic DNA from cultures, and so extreme care must be taken to keep reactions uncontaminated and in preventing cross-contamination.
Jana M. U'Ren +1 more
openaire +2 more sources
Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications
Advanced Science, EarlyView.Classical transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type‐specific programmability. This study presents calcium shock (CaSh), a method that dramatically improves particle delivery into single cells, colonies, and organoids.
Nicole Vo +12 more
wiley +1 more source
Phytobiomes Journal, 2023 The effects that mycobiomes have on physiological traits in turfgrasses are poorly understood. Drought tolerance, an economically and ecologically important trait, can be influenced by symbiotic fungi.
Glen Groben +8 more
doaj +1 more source
Phylogenomic analysis of lactobacillus curvatus reveals two lineages distinguished by genes for fermenting plant-derived carbohydrates [PDF]
, 2018 Lactobacillus curvatus is a lactic acid bacterium encountered in many different types of fermented food (meat, seafood, vegetables, and cereals). Although this species plays an important role in the preservation of these foods, few attempts have been ...
Chaillou, Stéphane +5 more
core +3 more sources
Advanced Science, EarlyView.A real‐world model of structured animal product restriction practiced for religious reasons reveals the dynamic adaptability of the human gut microbiome to dietary change and uncovers reductions in diversity and rare taxa loss. Integrated microbiome, metabolomic, and proteomic analyses uncover coordinated taxonomic and molecular shifts and identify ...
Christina Emmanouil +7 more
wiley +1 more source
Advancing molecular macrobenthos biodiversity monitoring: a comparison between Oxford Nanopore and Illumina based metabarcoding and metagenomics [PDF]
PeerJDNA-based methods and developments of sequencing technologies are integral to macrobenthos biodiversity studies, and their implementation as standardized monitoring methods is approaching.
Karlijn Doorenspleet +8 more
doaj +2 more sources
MaxSSmap: A GPU program for mapping divergent short reads to genomes with the maximum scoring subsequence [PDF]
, 2014 Programs based on hash tables and Burrows-Wheeler are very fast for mapping short reads to genomes but have low accuracy in the presence of mismatches and gaps.
Roshan, Usman, Turki, Turki
core +2 more sources
Advanced Science, EarlyView.A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son +2 more
wiley +1 more source
mSystems, 2019 Amplification, sequencing, and analysis of the 16S rRNA gene affords characterization of microbial community composition. As this tool has become more popular and amplicon-sequencing applications have grown in the total number of samples, growth in ...
Johanna B. Holm +13 more
doaj +3 more sources