Results 51 to 60 of about 142,852 (247)

Sample Preparation for Illumina MiSeq Dual Index Amplicon Sequencing v1 [PDF]

, 2019
Amplicon sequencing sample preparation for bacteria and microeukaryotes
Máté Vass, Anna Székely
openaire   +2 more sources

Clinical metagenomic sequencing - CSF RNA and DNA Illumina MiSeq v1

, 2022
This protocol was developed to evaluate unbiased cerebrospinal fluid metagenomics at a referral centre in South Africa. Commercial kit protocols are generally used unmodified and limited use of in-house developed methods or reagents is made. This maintains the simplicity and accessibility of the protocol which can serve as a base for evaluation of ...
Gert Marais, Ziyaad Valley-Omar, Kathleen Bateman, Kate McMullen, Dieter van der Westhuizen, Moepeng Maseko, Suzaan Marais, Diana Hardie, Adrian Brink   +8 more
openaire   +1 more source

Metagenomic data of free cyanide and thiocyanate degrading bacterial communities

Data in Brief, 2017
The data presented in this article contains the bacterial community structure of the free cyanide (CN-) and thiocyanate (SCN-) degrading organisms that were isolated from electroplating wastewater and synthetic SCN- containing wastewater.
Lukhanyo Mekuto, Seteno K.O. Ntwampe, John B.N. Mudumbi, Enoch A. Akinpelu, Maxwell Mewa-Ngongang   +4 more
doaj   +1 more source

A metagenomic survey of lamb's pre- and post-weaning fecal microbiomes

Journal of Taibah University for Science, 2020
Weaning is known to be the most stressful and critical transition experienced by lambs, it affects the structure of the gastrointestinal tract microbiomes, which significantly influences the lamb’s health and performance. The present study identified the
N. N. Albakri, N. A. Bouqellah, I. I. Shabana   +2 more
doaj   +1 more source

Environmental DNA (eDNA) 16S metabarcoding Illumina MiSeq NGS PCR Protocol v2 [PDF]

, 2018
This protocol is aimed at amplifying the 16S rRNA hypervariable region 4 (16S V4) in prokaryotes. The primers (515fBand 806rB, 926R) used in this protocol are based on the primer utilized in Apprill et al 2015 and the Earth Microbiome Project (EMP). This work was supported by NASA grant NNX14AP62A ‘National Marine Sanctuaries as Sentinel Sites for a ...
Collin Closek, Anni Djurhuus, Katie Pitz, Ryan Kelly, Reiko Michisaki, Kristine Walz, Hilary Starks, Francisco Chavez, Alexandria Boehm, Mya Breitbart   +9 more
openaire   +2 more sources

Mycobiome Analysis of Tall Fescue Grass Under Drought Stress Using the Illumina MiSeq and Oxford Nanopore Technology MinION

Phytobiomes Journal, 2023
The effects that mycobiomes have on physiological traits in turfgrasses are poorly understood. Drought tolerance, an economically and ecologically important trait, can be influenced by symbiotic fungi.
Glen Groben, Bruce B. Clarke, Lee J. Kerkhof, Stacy A. Bonos, William A. Meyer, Yuanshuo Qu, Jing Luo, Emily Walsh, Ning Zhang   +8 more
doaj   +1 more source

Nanopore duplex sequencing as an alternative to Illumina MiSeq sequencing for eDNA-based biomonitoring of coastal aquaculture impacts

Metabarcoding and Metagenomics
Oxford Nanopore Technologies has recently launched a duplex sequencing strategy and announced an improved error rate which is in a similar order of magnitude as Illumina sequencing.
Thorsten Stoeck, S. Katzenmeier, H. Breiner, Verena Rubel   +3 more
semanticscholar   +1 more source

Illumina MiSeq Dual-barcoded Two-step PCR Amplicon Sequencing Protocol v1

, 2020
Background Information High throughput amplicon sequencing is an extremely sensitive technique. The lab environment is full of airborne bacterial and fungal cells as well as bacterial and fungal genomic DNA from cultures, and so extreme care must be taken to keep reactions uncontaminated and in preventing cross-contamination.
Jana M. U'Ren, not provided A. Elizabeth Arnold   +1 more
openaire   +2 more sources

Patient‐specific pharmacogenomics demonstrates xCT as predictive therapeutic target in colon cancer with possible implications in tumor connectivity

Molecular Oncology, EarlyView.
This study integrates transcriptomic profiling of matched tumor and healthy tissues from 32 colorectal cancer patients with functional validation in patient‐derived organoids, revealing dysregulated metabolic programs driven by overexpressed xCT (SLC7A11) and SLC3A2, identifying an oncogenic cystine/glutamate transporter signature linked to ...
Marco Strecker, Keren Zohar, Martin Böttcher, Thomas Wartmann, Henry Freudenstein, Maximilian Doelling, Mihailo Andric, Wenjie Shi, Or Kakhlon, Katrin Hippe, Beatrix Jahnke, Dimitrios Mougiakakos, Franziska Baenke, Daniel Stange, Roland S. Croner, Michal Linial, Ulf D. Kahlert   +16 more
wiley   +1 more source

Advancing molecular macrobenthos biodiversity monitoring: a comparison between Oxford Nanopore and Illumina based metabarcoding and metagenomics [PDF]

PeerJ
DNA-based methods and developments of sequencing technologies are integral to macrobenthos biodiversity studies, and their implementation as standardized monitoring methods is approaching.
Karlijn Doorenspleet, Amalia Aikaterini Mailli, Berry B. van der Hoorn, Kevin K. Beentjes, Annelies De Backer, Sofie Derycke, Albertinka J. Murk, Henning Reiss, Reindert Nijland   +8 more
doaj   +2 more sources