Gut microbiome profiling of neonates using Nanopore MinION and Illumina MiSeq sequencing [PDF]
Frontiers in Microbiology, 2023 This study aimed to evaluate the difference in gut microbiomes between preterm and term infants using third-generation long-read sequencing (Oxford Nanopore Technologies, ONT) compared with an established gold standard, Illumina (second-generation short ...
Teahyen Cha +8 more
doaj +4 more sources
Illumina MiSeq sequencing analysis of fungal diversity in stored dates [PDF]
BMC Microbiology, 2017 Background Date palm has been a major fruit tree in the Middle East over thousands of years, especially in the Arabian Peninsula. Dates are consumed fresh (Rutab) or after partial drying and storage (Tamar) during off-season.
Ismail M. Al-Bulushi +4 more
doaj +5 more sources
Illumina MiSeq Sequencing for Preliminary Analysis of Microbiome Causing Primary Endodontic Infections in Egypt [PDF]
International Journal of Microbiology, 2018 The use of high throughput next generation technologies has allowed more comprehensive analysis than traditional Sanger sequencing. The specific aim of this study was to investigate the microbial diversity of primary endodontic infections using Illumina ...
Sally Ali Tawfik +3 more
doaj +5 more sources
Assessment of seawater bacterial infection in rabbit tibia by Illumina MiSeq sequencing and bacterial culture [PDF]
Journal of Orthopaedic Surgery and Research, 2021 Objectives We aimed to explore the bacterial community composition following ocean bacterial infection using an animal model. Methods This animal-based experiment was conducted from September 2019 to November 2019. Eighteen seawater filter membranes were
Du Wang +6 more
doaj +5 more sources
Comparison of Oxford Nanopore Technologies and Illumina MiSeq sequencing with mock communities and agricultural soil [PDF]
Scientific Reports, 2023 Illumina MiSeq is the current standard for characterizing microbial communities in soil. The newer alternative, Oxford Nanopore Technologies MinION sequencer, is quickly gaining popularity because of the low initial cost and longer sequence reads ...
Bo Maxwell Stevens +3 more
doaj +4 more sources
Comparison of SARS-CoV-2 sequencing using the ONT GridION and the Illumina MiSeq [PDF]
BMC Genomics, 2022 Background Over 4 million SARS-CoV-2 genomes have been sequenced globally in the past 2 years. This has been crucial in elucidating transmission chains within communities, the development of new diagnostic methods, vaccines, and antivirals.
Derek Tshiabuila +12 more
doaj +3 more sources
Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform. [PDF]
PLoS ONE, 2017 Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist.
Chongqing Wen +10 more
doaj +6 more sources
Illumina MiSeq sequencing disfavours a sequence motif in the GFP reporter gene. [PDF]
Sci Rep, 2016 AbstractGreen fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence.
Van den Hoecke S, Verhelst J, Saelens X.
europepmc +5 more sources
Insights into bacterioplankton community structure from Sundarbans mangrove ecoregion using Sanger and Illumina MiSeq sequencing approaches: A comparative analysis [PDF]
Genomics Data, 2017 Next generation sequencing using platforms such as Illumina MiSeq provides a deeper insight into the structure and function of bacterioplankton communities in coastal ecosystems compared to traditional molecular techniques such as clone library approach ...
Anwesha Ghosh, Punyasloke Bhadury
doaj +2 more sources
Illumina MiSeq sequencing reveals the effects of grape seed procyanidin on rumen archaeal communities in vitro. [PDF]
Asian-Australas J Anim Sci, 2020 Objective The present study explored the effects of grape seed procyanidin extract (GSPE) on rumen fermentation, methane production and archaeal communities in vitro. Methods A completely randomized experiment was conducted with in vitro incubation in a control group (CON, no GSPE addition; n = 9) and the treatment group (GSPE, 1 mg/bottle GSPE, 2 g/kg
Zhang H +4 more
europepmc +5 more sources