Results 31 to 40 of about 92,631 (141)
Scientific Reports, 2022 Well-defined molecular resistance markers are available for a range of antimalarial drugs, and molecular surveillance is increasingly important for monitoring antimalarial drug resistance.
Chanon Kunasol +6 more
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MethodsX, 2023 During the last years, the application of next-generation sequencing (NGS) technologies to search for specific genetic markers has become a crucial method for the characterization of microbial communities.
Alexandra Coello-Camba +2 more
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Analysis, optimization and verification of Illumina-generated 16S rRNA gene amplicon surveys. [PDF]
PLoS ONE, 2014 The exploration of microbial communities by sequencing 16S rRNA genes has expanded with low-cost, high-throughput sequencing instruments. Illumina-based 16S rRNA gene sequencing has recently gained popularity over 454 pyrosequencing due to its lower ...
Michael C Nelson +4 more
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Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform [PDF]
Nucleic Acids Research, 2015 With read lengths of currently up to 2 × 300 bp, high throughput and low sequencing costs Illumina's MiSeq is becoming one of the most utilized sequencing platforms worldwide. The platform is manageable and affordable even for smaller labs. This enables quick turnaround on a broad range of applications such as targeted gene sequencing, metagenomics ...
Schirmer, Melanie +5 more
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Choosing a benchtop sequencing machine to characterise Helicobacter pylori genomes. [PDF]
PLoS ONE, 2013 The fully annotated genome sequence of the European strain, 26695 was first published in 1997 and, in 1999, it was directly compared to the USA isolate J99, promoting two standard laboratory isolates for Helicobacter pylori (H. pylori) research. With the
Timothy T Perkins +3 more
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Phasing amplicon sequencing on Illumina Miseq for robust environmental microbial community analysis [PDF]
BMC Microbiology, 2015 Although high-throughput sequencing, such as Illumina-based technologies (e.g. MiSeq), has revolutionized microbial ecology, adaptation of amplicon sequencing for environmental microbial community analysis is challenging due to the problem of low base diversity.A new phasing amplicon sequencing approach (PAS) was developed by shifting sequencing phases
Wu, Liyou +9 more
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BMC Genomics, 2023 Background Illumina sequencing platform requires base diversity in the initial 11 cycles for efficient cluster identification and colour matrix estimation. This limitation yields low-quality data for amplicon libraries having homogeneous base composition.
Tejali Naik +4 more
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Viruses, 2020 Enteroviruses are small RNA viruses that affect millions of people each year by causing an important burden of disease with a broad spectrum of symptoms.
Carole Grädel +7 more
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A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq [PDF]
PeerJ, 2016 Background The bacterial 16S rRNA gene has historically been used in defining bacterial taxonomy and phylogeny. However, there are currently no high-throughput methods to sequence full-length 16S rRNA genes present in a sample with precision.
Catherine M. Burke, Aaron E. Darling
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mSystems, 2019 Amplification, sequencing, and analysis of the 16S rRNA gene affords characterization of microbial community composition. As this tool has become more popular and amplicon-sequencing applications have grown in the total number of samples, growth in ...
Johanna B. Holm +13 more
doaj +3 more sources