Saliva microbiome profiling by full-gene 16S rRNA Oxford Nanopore Technology versus Illumina MiSeq sequencing [PDF]
npj Biofilms and MicrobiomesMolecular characterization of the oral microbiome is a crucial first step in experiments which aim to understand the complex dynamics of the oral microbiome or the interplay with host health and disease.
Anders Esberg +3 more
doaj +2 more sources
Development of microsatellite markers using Illumina MiSeq sequencing to characterize Ephedra gerardiana (Ephedraceae). [PDF]
Appl Plant Sci, 2017 Premise of the study:Ephedra gerardiana (Ephedraceae), occurring in the Himalayan ranges, is an important plant species used in Tibetan medicine. Due to the lack of molecular markers to characterize genetic diversity, knowledge for conservation and uses of E. gerardiana resources is limited; we therefore developed microsatellite markers for use in this
De J, Zhu W, Liu T, Wang Z, Zhong Y.
europepmc +3 more sources
Characterization of Microsatellite Markers for Pinedrops, Pterospora andromedea (Ericaceae), from Illumina MiSeq sequencing [PDF]
Applications in Plant Sciences, 2014 Premise of the study: Pterospora andromedea (Ericaceae) is a mycoheterotrophic plant endemic to North America with a disjunct distribution. Eastern populations are in decline compared to western populations.
Lisa C. Grubisha +4 more
doaj +3 more sources
Illumina MiSeq and iSeq platforms yield comparable results for viral genomic sequencing
Journal of Virological MethodsIllumina MiSeq and iSeq are widely used short-read next-generation sequencing (NGS) platforms. The MiSeq instrument is specialized for mid-range throughput, while the iSeq instrument has lower throughput but is less expensive and simpler to operate. Several studies have compared Illumina platforms for sequencing of a variety of specimen types, but very
Darlene D Wagner +2 more
exaly +3 more sources
A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers [PDF]
BMC Genomics, 2012 Background Next generation sequencing (NGS) technology has revolutionized genomic and genetic research. The pace of change in this area is rapid with three major new sequencing platforms having been released in 2011: Ion Torrent’s PGM, Pacific ...
Quail Michael A +8 more
doaj +4 more sources
Assessment of PHRED Score Characteristics in Illumina MiSeq Amplicon Sequencing. [PDF]
J Comput BiolPHRED scores are confidence values associated with each basecall generated by sequencers. The score is defined as a monotonic function of the probability that the basecall is incorrect. The calibration of PHRED scores has previously been examined by evaluating errors made in reading known sequences.
Sims S, Khudyakov Y, Zelikovsky A.
europepmc +2 more sources
Development and assessment of an optimized next-generation DNA sequencing approach for the mtgenome using the Illumina MiSeq [PDF]
Forensic Science International: Genetics, 2014 The development of molecular tools to detect and report mitochondrial DNA (mtDNA) heteroplasmy will increase the discrimination potential of the testing method when applied to forensic cases. The inherent limitations of the current state-of-the-art, Sanger-based sequencing, including constrictions in speed, throughput, and resolution, have hindered ...
Jennifer A Mcelhoe +2 more
exaly +3 more sources
Identification of the Microbiota in Coconut Water, Kefir, Coconut Water Kefir and Coconut Water Kefir-Fermented Sourdough Using Culture-Dependent Techniques and Illumina–MiSeq Sequencing [PDF]
MicroorganismsMansi Limbad +2 more
exaly +2 more sources
Illumina DNA Prep (M) Tagmentation Library Preparation for use on an Illumina MiSeq Sequencer v1 [PDF]
, 2020 This procedure outlines the protocol for whole genome sequencing of bacterial organisms using the Illumina DNA Prep library preparation kit for sequencing on an Illumina MiSeq sequencer. This document applies to all laboratory personnel in the Division of Microbiology (DM) as well as laboratories in the GenomeTrakr Network.
Julie Haendiges +3 more
+5 more sources
Effects of wheat root exudates on bacterial communities in the rhizosphere of watermelon
Plant, Soil and Environment, 2021 In this study, we investigated the effects of wheat root exudates on soil bacterial communities in the watermelon rhizosphere using quantitative PCR and Illumina MiSeq sequencing.
Jibo Shi +5 more
doaj +1 more source