Results 51 to 60 of about 92,631 (141)

Rapid and economical drug resistance profiling with Nanopore MinION for clinical specimens with low bacillary burden of Mycobacterium tuberculosis

BMC Research Notes, 2020
Objective We designed and tested a Nanopore sequencing panel for direct tuberculosis drug resistance profiling. The panel targeted 10 resistance-associated loci.
Wai Sing Chan, Chun Hang Au, Yvonne Chung, Henry Chi Ming Leung, Dona N. Ho, Elaine Yue Ling Wong, Tak Wah Lam, Tsun Leung Chan, Edmond Shiu Kwan Ma, Bone Siu Fai Tang   +9 more
doaj   +1 more source

Development and Validation of a Next-Generation Sequencing Assay for BRCA1 and BRCA2 Variants for the Clinical Laboratory. [PDF]

PLoS ONE, 2015
The objective of this study was to design and validate a next-generation sequencing assay (NGS) to detect BRCA1 and BRCA2 mutations. We developed an assay using random shearing of genomic DNA followed by RNA bait tile hybridization and NGS sequencing on ...
Charles M Strom, Steven Rivera, Christopher Elzinga, Taraneh Angeloni, Sun Hee Rosenthal, Dana Goos-Root, Martin Siaw, Jamie Platt, Cory Braastadt, Linda Cheng, David Ross, Weimin Sun   +11 more
doaj   +1 more source

A quantitative and qualitative comparison of illumina MiSeq and 454 amplicon sequencing for genotyping the highly polymorphic major histocompatibility complex (MHC) in a non-model species

BMC Research Notes, 2017
Background High-throughput sequencing enables high-resolution genotyping of extremely duplicated genes. 454 amplicon sequencing (454) has become the standard technique for genotyping the major histocompatibility complex (MHC) genes in non-model organisms.
Haslina Razali, Emily O’Connor, Anna Drews, Terry Burke, Helena Westerdahl   +4 more
doaj   +1 more source

Whole Genome Sequencing of Influenza A and B Viruses With the MinION Sequencer in the Clinical Setting: A Pilot Study

Frontiers in Microbiology, 2018
Introduction: Whole genome sequencing (WGS) of influenza viruses is important for preparing vaccines and coping with newly emerging viruses. However, WGS is difficult to perform using conventional next-generation sequencers in developing countries, where
Kazuo Imai, Kazuo Imai, Kazuo Imai, Kaku Tamura, Tomomi Tanigaki, Mari Takizawa, Eiko Nakayama, Takahiko Taniguchi, Misako Okamoto, Yasumasa Nishiyama, Norihito Tarumoto, Norihito Tarumoto, Kotaro Mitsutake, Kotaro Mitsutake, Takashi Murakami, Takashi Murakami, Shigefumi Maesaki, Shigefumi Maesaki, Takuya Maeda, Takuya Maeda   +19 more
doaj   +1 more source

Identifying Bacterial Airways Infection in Stable Severe Asthma Using Oxford Nanopore Sequencing Technologies

Microbiology Spectrum, 2022
Previous metagenomic studies in asthma have been limited by inadequate sequencing depth for species-level bacterial identification and by heterogeneity in clinical phenotyping.
Maisha F. Jabeen, Nicholas D. Sanderson, Dona Foster, Derrick W. Crook, Jennifer L. Cane, Catherine Borg, Clare Connolly, Samantha Thulborn, Ian D. Pavord, Paul Klenerman, Teresa L. Street, Timothy S. C. Hinks   +11 more
doaj   +1 more source

Datasets of surface water microbial populations from two anthropogenically impacted sites on the Bhagirathi-Hooghly River

Data in Brief, 2020
The Bhagirathi-Hooghly River, part of the River Ganga, flows along densely urbanized areas in West Bengal, India. The River water is extensively used for household activities, human consumption including bathing, social purposes and multifaceted ...
Anwesha Ghosh, Manojit Debnath, Punyasloke Bhadury   +2 more
doaj   +1 more source

Chromosome HeatMap in CDK Patients as Defined by Multiregional Sequencing on Illumina MiSeq Platform

European Journal of Medical and Health Sciences, 2020
Renal failure and kidney disease are major concerns worldwide and are commonly coupled to diseases like hypertension, diabetes, obesity, and hypercholesterolemia. We undertook this study to explore the scope of genetic spectrum underlying the physiopathology of end-stage renal disease (ESRD) using whole exome sequencing (WES) on genomic DNA (gDNA) from
Mohammad F. Fazaludeen, Aymen A. Warille, Mohd Ibrahim Alaraj, Edem Nuglozeh   +3 more
openaire   +1 more source

Full genome virus detection in fecal samples using sensitive nucleic acid preparation, deep sequencing, and a novel iterative sequence classification algorithm.

PLoS ONE, 2014
We have developed a full genome virus detection process that combines sensitive nucleic acid preparation optimised for virus identification in fecal material with Illumina MiSeq sequencing and a novel post-sequencing virus identification algorithm ...
Matthew Cotten, Bas Oude Munnink, Marta Canuti, Martin Deijs, Simon J Watson, Paul Kellam, Lia van der Hoek   +6 more
doaj   +1 more source

Mycobiome Analysis of Tall Fescue Grass Under Drought Stress Using the Illumina MiSeq and Oxford Nanopore Technology MinION

Phytobiomes Journal, 2023
The effects that mycobiomes have on physiological traits in turfgrasses are poorly understood. Drought tolerance, an economically and ecologically important trait, can be influenced by symbiotic fungi.
Glen Groben, Bruce B. Clarke, Lee J. Kerkhof, Stacy A. Bonos, William A. Meyer, Yuanshuo Qu, Jing Luo, Emily Walsh, Ning Zhang   +8 more
doaj   +1 more source

Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform [PDF]

Applied and Environmental Microbiology, 2013
ABSTRACT Rapid advances in sequencing technology have changed the experimental landscape of microbial ecology. In the last 10 years, the field has moved from sequencing hundreds of 16S rRNA gene fragments per study using clone libraries to the sequencing of millions of fragments per study using next-generation sequencing technologies ...
James J, Kozich, Sarah L, Westcott, Nielson T, Baxter, Sarah K, Highlander, Patrick D, Schloss   +4 more
openaire   +2 more sources