Results 71 to 80 of about 92,631 (141)

Understanding and mitigating some limitations of Illumina© MiSeq for environmental sequencing of Fungi [PDF]

, 2017
Abstract ITS-amplicon metabarcode studies using the illumina MiSeq sequencing platform are the current standard tool for fungal ecology studies. Here we report on some of the particular challenges experienced while creating and using a ribosomal RNA gene (rDNA) amplicon library for an ecological study. Two significant complications were
Thomas, Dan, Vandegrift, Roo, Bailes, Graham, Roy, Bitty   +3 more
openaire   +1 more source

An improved and extended dual-index multiplexed 16S rRNA sequencing for the Illumina HiSeq and MiSeq platform

BMC Genomic Data
Background Recent advancements in next-generation sequencing (NGS) technology have ushered in significant improvements in sequencing speed and data throughput, thereby enabling the simultaneous analysis of a greater number of samples within a single ...
A.K. Larin, K.M. Klimina, V.A. Veselovsky, E.I. Olekhnovich, M.D. Morozov, D.I. Boldyreva, R.A. Yunes, A.I. Manolov, D.E. Fedorov, A.V. Pavlenko, Y.S. Galeeva, E.V. Starikova, E.N. Ilina   +12 more
doaj   +1 more source

Genomic analyses of multidrug-resistant Salmonella Indiana, Typhimurium, and Enteritidis isolates using MinION and MiSeq sequencing technologies.

PLoS ONE, 2020
We sequenced 25 isolates of phenotypically multidrug-resistant Salmonella Indiana (n = 11), Typhimurium (n = 8), and Enteritidis (n = 6) using both MinION long-read [SQK-LSK109 and flow cell (R9.4.1)] and MiSeq short-read (Nextera XT and MiSeq Reagent ...
Zhao Chen, Dai Kuang, Xuebin Xu, Narjol González-Escalona, David L Erickson, Eric Brown, Jianghong Meng   +6 more
doaj   +1 more source

Illumina MiSeq sequencing investigation of Chanhua (Cordyceps cicadae Shing) fungal community structures in different regions

Journal of Traditional Chinese Medical Sciences, 2018
Objective: This study was carried out to elucidate the fungal diversity of Chanhua (Cordyceps cicadae Shing), which is an entomogenous fungal parasite that grows on wingless cicada nymphs.
Tengfei Liu, Hongmei Zhang, Xiaosa Shi, Weiwei Ma, Yancong Zhang, Ziyao Liu, Ying Huang, Xinyuan Shi   +7 more
doaj   +1 more source

Microbial genome (Illumina MiSeq) sequencing of drinking water treatment residuals to evaluate compatibility with environmental applications. [PDF]

Environ Monit Assess, 2023
Turner T, Tonge D, Glanville HC, Wheeler R, Oliver IW.   +4 more
europepmc   +1 more source

Isolation and characterization of novel microsatellite loci for the Eastern Pacific marine sponge Mycale cecilia by Illumina MiSeq sequencing. [PDF]

Mol Biol Rep, 2023
Hernández-Lozano MY, Cruz-Barraza JA, Rocha-Olivares A.   +2 more
europepmc   +1 more source

illumina MiSeq Dual Index Amplicon Sequencing Sample Preparation v1

, 2019
Preparation of PCR products for amplicon sequencing
openaire   +1 more source

On the ability to extract MLVA profiles of Vibrio cholerae isolates from WGS data generated with Oxford Nanopore Technologies

BMC Research Notes
Objective Multiple-Locus Variable Number of Tandem Repeats (VNTR) Analysis (MLVA) is widely used to subtype pathogens causing foodborne and waterborne disease outbreaks.
Jérôme Ambroise, Bertrand Bearzatto, Jean-Francois Durant, Leonid M. Irenge, Jean-Luc Gala   +4 more
doaj   +1 more source

Effect of Processing Treatment and Modified Atmosphere Packing on Carrot's Microbial Community Structure by Illumina MiSeq Sequencing. [PDF]

Molecules, 2022
Ratajczak K, Staninska-Pięta J, Czarny J, Cyplik P, Wolko Ł, Piotrowska-Cyplik A.   +5 more
europepmc   +1 more source

OPTIMIZATION OF ILLUMINA® NEXTERA™ XT LIBRARY PREPARATION FOR THE MITOCHONDRIAL GENOME SEQUENCING AND CONFIRMATORY SANGER SEQUENCING

Medicinski Glasnik
Aim: Due to increasing use of mitochondrial DNA (mtDNA) sequencing in both forensic practice and clinical disease research, this study explores the optimization of the next-generation sequencing (NGS) method for whole mitochondrial genome analysis on the
Nejira Handžić, Dino Pećar, Selma Durgut, Naida Mulahuseinović, Ivana Čeko, Adna Ašić, Mirza Izmirlija, Sabina Šegalo, Lana Salihefendić, Rijad Konjhodžić   +9 more
doaj   +1 more source