Results 61 to 70 of about 92,631 (141)

Evaluation of strategies for the assembly of diverse bacterial genomes using MinION long-read sequencing

BMC Genomics, 2019
Background Short-read sequencing technologies have made microbial genome sequencing cheap and accessible. However, closing genomes is often costly and assembling short reads from genomes that are repetitive and/or have extreme %GC content remains ...
Sarah Goldstein, Lidia Beka, Joerg Graf, Jonathan L. Klassen   +3 more
doaj   +1 more source

Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices

BMC Genomics, 2018
Background Prosthetic joint infections are clinically difficult to diagnose and treat. Previously, we demonstrated metagenomic sequencing on an Illumina MiSeq replicates the findings of current gold standard microbiological diagnostic techniques ...
Nicholas D Sanderson, Teresa L Street, Dona Foster, Jeremy Swann, Bridget L Atkins, Andrew J Brent, Martin A McNally, Sarah Oakley, Adrian Taylor, Tim E A Peto, Derrick W Crook, David W Eyre   +11 more
doaj   +1 more source

German-Wide Interlaboratory Study Compares Consistency, Accuracy and Reproducibility of Whole-Genome Short Read Sequencing

Frontiers in Microbiology, 2020
We compared the consistency, accuracy and reproducibility of next-generation short read sequencing between ten laboratories involved in food safety (research institutes, state laboratories, universities and companies) from Germany and Austria ...
Laura Uelze, Maria Borowiak, Markus Bönn, Erik Brinks, Carlus Deneke, Thomas Hankeln, Sylvia Kleta, Larissa Murr, Kerstin Stingl, Kathrin Szabo, Simon H. Tausch, Anne Wöhlke, Burkhard Malorny   +12 more
doaj   +1 more source

16S Sequencing on Illumina MiSeq Platform v1

The 16S protocol detailed here is designed to amplify bacteria using V4-V5 hyper-variable regions of the 16S rRNA gene on the Illumina MiSeq DNA sequencer.
Angela Schmoldt, Melissa Schussman
openaire   +1 more source

Simultaneous and complete genome sequencing of influenza A and B with high coverage by Illumina MiSeq Platform

Journal of Virological Methods, 2013
Active global surveillance and characterization of influenza viruses are essential for better preparation against possible pandemic events. Obtaining comprehensive information about the influenza genome can improve our understanding of the evolution of influenza viruses and emergence of new strains, and improve the accuracy when designing preventive ...
Rutvisuttinunt, Wiriya, Chinnawirotpisan, Piyawan, Simasathien, Sriluck, Shrestha, Sanjaya K., Yoon, In-Kyu, Klungthong, Chonticha, Fernandez, Stefan   +6 more
openaire   +2 more sources

Nanopore duplex sequencing as an alternative to Illumina MiSeq sequencing for eDNA-based biomonitoring of coastal aquaculture impacts [PDF]

Metabarcoding and Metagenomics
Oxford Nanopore Technologies has recently launched a duplex sequencing strategy and announced an improved error rate which is in a similar order of magnitude as Illumina sequencing.
Thorsten Stoeck, Sven Nicolai Katzenmeier, Hans-Werner Breiner, Verena Rubel   +3 more
doaj   +3 more sources

Metagenomic data for Halichondria panicea from Illumina and nanopore sequencing and preliminary genome assemblies for the sponge and two microbial symbionts

BMC Research Notes, 2022
Objectives These data were collected to generate a novel reference metagenome for the sponge Halichondria panicea and its microbiome for subsequent differential expression analyses.
Brian W. Strehlow, Astrid Schuster, Warren R. Francis, Donald E. Canfield   +3 more
doaj   +1 more source

Whole-Genome Sequencing of Porcine Epidemic Diarrhea Virus by Illumina MiSeq Platform [PDF]

, 2016
Porcine epidemic diarrhea virus (PEDV) belongs to the genus Alphacoronavirus of the family Coronaviridae. PEDV was identified as an emerging pathogen in US pig populations in 2013. Since then, this virus has been detected in at least 31 states in the USA and has caused significant economic loss to the swine industry.
Wang, Leyi, Stuber, Tod, Camp, Patrick, Robbe-Austerman, Suelee, Zhang, Yan   +4 more
openaire   +1 more source

Comparative analysis of Illumina, PacBio, and nanopore for 16S rRNA gene sequencing of rabbit’s gut microbiota

Frontiers in Microbiomes
This research aims to assess whether sequencing the full length of the 16S rRNA gene using PacBio HiFi sequencing and Oxford Nanopore Technology (ONT) platforms outperform Illumina MiSeq platform in providing detailed specie level insights.
Iliyass Biada, María Antonia Santacreu, Oscar González-Recio, Noelia Ibáñez-Escriche   +3 more
doaj   +1 more source

Rabies Virus Sequencing using Illumina- MiSeq v1

This Rabies whole genome sequencing protocol has been derived and modified from the Illumina COVIDSeq RUO sequencing pipeline. The protocol has been modified and optimised for sequencing Rabies virus (RABV). The methodology uses RABV-specific primers that have been designed in-house.
Chakrakodi N Varun, Dhanya K, Ashwini M Ananda, Reeta Mani   +3 more
openaire   +1 more source