Results 131 to 140 of about 5,090,386 (280)

Oligonucleotide-polymer conjugation for imaging mass cytometry v2

, 2023
This protocol describes the procedures to label 5'-thiol modified oligonucleotides with maleimide carrying polymers which are also chelated with metal-ions. This is the basis to perform RNAscope with Imaging Mass Cytometry since therein we use metal readouts instead of fluorescence readouts. This protocol has been used in the following publications:
openaire   +1 more source

Getting the picture: The coming of age of imaging MS

, 2015
This retrospective on imaging mass spectrometry describes the coming of age of an approach that has matured from an innovative technological development to a broadly applied analytical tool.
Heeren, Ron M. A.
core   +1 more source

Electroactive 3D Porous Graphene Foam Impedimetric Biosensor for Ultrasensitive Detection of MCF‐7‐Cells‐Derived Extracellular Vesicles in Human Serum

Advanced Materials Technologies, EarlyView.
A label‐free impedimetric biosensor based on electroactive 3D porous graphene foam enables ultrasensitive and selective detection of CD81 protein and MCF‐7 cell‐derived extracellular vesicles in human serum. Detection limits of 0.048 fg/mL for CD81 and ∼24 EVs/mL are achieved, demonstrating a robust platform for selective, label‐free, and ...
Michael Robert Furness, Ashish Kalkal, Tia Wardlaw, Jessica Valli, Suresh Chandra Baral, Pablo Lozano Sanchez, Marco Caffio, Sudhagar Pitchaimuthu, Olga Oikonomidou, Jason Webber, Nick R. Leslie, Anitha Devadoss   +11 more
wiley   +1 more source

GPR124 Alleviates Blood–Brain Barrier Disruption by Enhancing Microvascular Endothelial Function after Traumatic Brain Injury

Advanced Science, EarlyView.
Our study reveals the protective role of GPR124 in maintaining BBB integrity and promoting neurological recovery following TBI. It makes a significant contribution by uncovering a novel molecular interaction between GPR124 and FGFBP1 and linking this to activation of the Wnt/β‐catenin signaling pathway in vascular repair mechanisms.
Chen Wang, Lin Cai, Qiuyuan Gong, Yang Yang, Yuqing Liang, Xinyu Niu, Lai Wei, Ze Liu, Shengju Wu, Yinghui Men, Yaohui Tang, Jun Ding, Hengli Tian, Hao Chen   +13 more
wiley   +1 more source

The phenogenomic landscapes of pleural mesothelioma tumor microenvironment predict clinical outcomes

Journal of Translational Medicine
Background Malignant pleural mesothelioma (MPM) is a rare and aggressive malignancy with limited therapeutic options. To improve patients management and treatment, more precise stratification strategies are needed.
Federica Torricelli, Filomena Spada, Cynthia Bishop, Katrina Todd, Daisuke Nonaka, Nedyalko Petrov, Manuela Terranova Barberio, Alan G. Ramsay, Richard Ellis, Alessia Ciarrocchi, Benedetta Apollonio, Andrea Billè   +11 more
doaj   +1 more source

Staining protocol for Imaging Mass Cytometry v1

In Imaging Mass Cytometry (IMC), a high-resolution laser is combined with a mass cytometer that permits mass spectrometry-based, spatially reserved high-dimensional analysis of intact formalin-fixed paraffin-embedded (FFPE) tissues. The protocol summarizes the staining procedure for IMC using a cocktail of heavy metal conjugated primary antibodies ...
Vijayakumar R Kakade, Tifanny Budiman, Lloyd Cantley   +2 more
openaire   +1 more source

A micropixelated ion-imaging detector for mass resolution enhancement of a QMS instrument

, 2015
An in-vacuum position-sensitive micropixelated detector (Timepix) is used to investigate the time-dependent spatial distribution of different charge state (and hence different mass-to-charge (m/z)) ions exiting an electrospray ionization (ESI)-based ...
Kistemaker, Piet, Heeren, Ron M. A., Maher, Simon, Syed, Sarfaraz U. A. H., Eijkel, Gert B., Taylor, Stephen   +5 more
core   +1 more source

CD9+ B Cells Induce T Follicular Helper Cell Apoptosis to Regulate Germinal Center Regression

Advanced Science, EarlyView.
A unique subset of CD9+ B cells regulates germinal center B (GC‐B) cell regression by inducing T follicular helper (Tfh) cell apoptosis through multiple signaling pathways, such as ALCAM‐CD6. FOXP1 plays a key role in GC‐B cell differentiation. These findings clarify mechanisms of GC degeneration and termination and offer potential targets for adenoid ...
Wenjing Liao, Lijuan Song, Meiqian Xu, Tianhao Liang, Yixuan Chen, Gui Chen, Zaihuan Lin, Huakang Du, Guofei Feng, Zhiming Feng, Qi Zhang, Fan Pan, Saixuan Yang, Xinyin Zhang, Wenxu Peng, Tianlong Li, Zhuoshen Lin, Yabang Chen, Jiayi Liu, Baoxin Peng, Yan Liu, Xing Niu, Ting Li, Chengyu Huang, Ke Mo, Jianlei Xie, Yu Zou, Xiaowen Zhang   +27 more
wiley   +1 more source

Mass cytometry: exploring the immune landscape of systemic autoimmune and inflammatory diseases in the past fourteen years

Frontiers in Immunology
Auto-immune and inflammatory diseases are heterogenous in their clinical manifestations and prognosis, even among individuals presenting with the same pathology. Understanding the immunological alterations involved in their pathogenesis provides valuable
Aïcha Kante, Aïcha Kante, Mathieu F. Chevalier, Damien Sène, Damien Sène, Jeanne Chauffier, Jeanne Chauffier, Stéphane Mouly, Stéphane Mouly, Benjamin Glenn Chousterman, Benjamin Glenn Chousterman, Fériel Azibani, Benjamin Terrier, Benjamin Terrier, Théo Pezel, Théo Pezel, Cloé Comarmond, Cloé Comarmond   +17 more
doaj   +1 more source

Mass Spectrometry Imaging for the Investigation of Intratumor Heterogeneity

, 2017
One of the big clinical challenges in the treatment of cancer is the different behavior of cancer patients under guideline therapy. An important determinant for this phenomenon has been identified as inter- and intratumor heterogeneity.
Balluff, B., Hanselmann, M., Heeren, R. M. A., Drake, RR, McDonnell, LA   +4 more
core   +1 more source