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Signal Amplification for Imaging Mass Cytometry
Bioconjugate Chemistry, 2019An enzyme-catalyzed reporter deposition stain has been developed for Imaging Mass Cytometry (IMC). The reagent consists of an alkaline phosphatase substrate tethered to a tellurophene which serves as reporter group for mass cytometry. Upon phosphate hydrolysis, a quinone methide is released which covalently labels local nucleophiles. This strategy is a
Rahul Rana +3 more
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DNA-Conjugated Gold Nanoparticles as High-Mass Probes in Imaging Mass Cytometry
ACS Applied Bio Materials, 2019We employ imaging mass cytometry (IMC) to investigate in vitro uptake and cellular distribution of DNA-functionalized gold nanoparticles (AuNPs). IMC enables the multiparametric imaging of cell components and allows for the detection of AuNPs in cells with >100 times higher sensitivity than conventional confocal fluorescence imaging, as each ...
Derek J Wilson +2 more
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Testing a Nanoparticle Reagent for Imaging Mass Cytometry
BiomacromoleculesMass cytometry (MC), a powerful single-cell analysis technique, has limitations in detecting low-abundance biomarkers. Nanoparticle (NP) reagents offer the potential for enhancing sensitivity by carrying large numbers of heavy metal isotopes. Here, we report NP reporters for imaging mass cytometry (IMC) based on NaYF4:Yb3+/Er3+ NPs.
Mahtab Abtahi +9 more
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Chemical Engineering JournalImaging mass cytometry - elemental immunohistochemistry for multiparametric imaging and quantitation
The Journal of Immunology, 2016Abstract Pathology assessment of tissue sections provides prognostic evaluation and helps select optimum treatment regimens. Digital pathology has made significant strides in the analysis of several markers. There is a growing understanding of cell heterogeneity within tumor tissue, role of microenvironment, and the impact of immune ...
Olga Ornatsky +6 more
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Imaging Mass Cytometry for In Situ Immune Profiling
The complexities and cellular heterogeneity associated with tissues necessitate the concurrent detection of markers beyond the limitations of conventional imaging approaches in order to spatially resolve the relationships between immune cell populations and their environments.Kevin, Hu +2 more
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Spatial immunophenotyping of FFPE tissues by imaging mass cytometry
The immune compartment of a tissue is dynamic, changing to respond to infections, tumors, or therapeutic interventions. Within tissues, local microenvironments provide interaction partners and cytokines that can gear immune cells into distinct functional states. Thus, it is not just the immune composition of a tissue, but also the relative localizationCarolina, Mangana, Barbara B, Maier
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Enhancing Mass-Cytometry Imaging Analysis
Inquiry@Queen's Undergraduate Research Conference ProceedingsMass cytometry imaging is a technique that utilizes mass spectrometry instead of fluorescent flow cytometry to detect metal-conjugated antibodies binding to single cell antigens. This method generates multi-dimensional images, a complex data type with significant computational and interpretation challenges due to the absence of a standardized analysis ...
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