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Ruthenium counterstaining for imaging mass cytometry
Journal of Pathology, 2018 AbstractImaging mass cytometry is a novel imaging modality that enables simultaneous antibody‐based detection of >40 epitopes and molecules in tissue sections at subcellular resolution by the use of isotopically pure metal tags. Essential for any imaging approach in which antigen detection is performed is counterstaining, which reveals the overall ...
Bernd Bodenmiller +2 more
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Mass Cytometry, Imaging Mass Cytometry, and Multiplexed Ion Beam Imaging Use in a Clinical Setting
Clinics in Laboratory Medicine, 2021Mass cytometry (MC), imaging mass cytometry (IMC), and multiplexed ion beam imaging (MIBI) represent a new generation of tools to understand increasingly complex systems. Although these technologies differ in their intended applications, with MC being most similar to flow cytometry, and IMC/MIBI being similar to immunohistochemistry, they all share a ...
Raymond Devine
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Signal Amplification for Imaging Mass Cytometry
Bioconjugate Chemistry, 2019 An enzyme-catalyzed reporter deposition stain has been developed for Imaging Mass Cytometry (IMC). The reagent consists of an alkaline phosphatase substrate tethered to a tellurophene which serves as reporter group for mass cytometry. Upon phosphate hydrolysis, a quinone methide is released which covalently labels local nucleophiles. This strategy is a
Rahul Rana +3 more
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DNA-Conjugated Gold Nanoparticles as High-Mass Probes in Imaging Mass Cytometry
ACS Applied Bio Materials, 2019We employ imaging mass cytometry (IMC) to investigate in vitro uptake and cellular distribution of DNA-functionalized gold nanoparticles (AuNPs). IMC enables the multiparametric imaging of cell components and allows for the detection of AuNPs in cells with >100 times higher sensitivity than conventional confocal fluorescence imaging, as each ...
Derek Wilson +2 more
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Enhancing Mass-Cytometry Imaging Analysis
Inquiry@Queen's Undergraduate Research Conference ProceedingsMass cytometry imaging is a technique that utilizes mass spectrometry instead of fluorescent flow cytometry to detect metal-conjugated antibodies binding to single cell antigens. This method generates multi-dimensional images, a complex data type with significant computational and interpretation challenges due to the absence of a standardized analysis ...
Tuitt-Barnes, Dream
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Automated Cell Phenotyping for Imaging Mass Cytometry
2022 44th Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC), 2022Imaging mass cytometry (IMC) is a new advancement in tissue imaging that is quickly gaining wider usage since its recent launch. It improves upon current tissue imaging methods by allowing for a significantly higher number of proteins to be imaged at once on a single tissue slide. For most analyses of IMC data, determining the phenotype of each cell is
Sindhura Thirumal +4 more
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Imaging Mass Cytometry in Immuno-Oncology
2023In situ profiling of the tumor-immune microenvironment (TiME) requires the ability to co-localize and detect multiple proteins simultaneously. Imaging mass cytometry (IMC), using the Hyperion™ imaging system is a novel multiplex imaging modality that currently enables detection of up to 50 markers on fixed tissues at subcellular resolution and thus has
Tiziana, Cotechini +2 more
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Testing a Nanoparticle Reagent for Imaging Mass Cytometry
BiomacromoleculesMass cytometry (MC), a powerful single-cell analysis technique, has limitations in detecting low-abundance biomarkers. Nanoparticle (NP) reagents offer the potential for enhancing sensitivity by carrying large numbers of heavy metal isotopes. Here, we report NP reporters for imaging mass cytometry (IMC) based on NaYF4:Yb3+/Er3+ NPs.
Mahtab Abtahi +9 more
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Imaging mass cytometry - elemental immunohistochemistry for multiparametric imaging and quantitation
The Journal of Immunology, 2016Abstract Pathology assessment of tissue sections provides prognostic evaluation and helps select optimum treatment regimens. Digital pathology has made significant strides in the analysis of several markers. There is a growing understanding of cell heterogeneity within tumor tissue, role of microenvironment, and the impact of immune ...
Olga Ornatsky +6 more
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