Results 61 to 70 of about 89,290 (305)

Unrearranged immunoglobulin variable region genes have a functional promoter

Nucleic Acids Research, 1982
We have tested whether immunoglobulin light chain variable region genes are capable of directing initiation of transcription without undergoing the DNA rearrangement which creates a complete immunoglobulin gene. Two human Vk genes specifically initiated transcription in vitro at a site which is approximately 30 bases downstream of a TATA box and 20 ...
D L, Bentley, P J, Farrell, T H, Rabbitts   +2 more
openaire   +3 more sources

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Combinatorial functions of two chimeric antibodies directed to human CD4 and one directed to the a-chain of the human interleukin-2 receptor [PDF]

, 1992
The general feasibility of chimerization of monoclonal antibodies (mAbs) has already been shown for a large number of them. In order to evaluate in vitro parameters relevant to immunosuppressive therapy, we have chimerized and synthesized two anti-CD4 ...
Schwirzke, Marina, Kaluzab, Brigitte, Reiter, Christian, Rieber, Ernst Peter, Scheuer, Werner, Flieger, Dimitri, Lenz, Helmut, Weissenhorn, Winfried, Weiss, Elisabeth H., Weidle, Ulrich H., Riethmüller, Gert   +10 more
core   +1 more source

A comprehensive analysis of immunoglobulin heavy chain genes in the Bactrian camel (Camelus bactrianus) [PDF]

Frontiers of Agricultural Science and Engineering, 2015
Heavy chain only antibodies (HCAbs) represent a rare type of antibody that is devoid of light chains and the CH1 domain that have been reported in cartilaginous fish and camelids.
Zuoxiang LIANG,Tao WANG,Yi SUN,Wenlong YANG,Zhihong LIU,Jing FEI,Ying GUO,Qingwei MA,Qingjie PAN,Liming REN
doaj   +1 more source

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source

Immunoglobulin secretion influences the composition of chicken caecal microbiota

Scientific Reports
The chicken caecum is colonised by hundreds of different bacterial species. Which of these are targeted by immunoglobulins and how immunoglobulin expression shapes chicken caecal microbiota has been addressed in this study. Using cell sorting followed by
Jiri Volf, Bernd Kaspers, Benjamin Schusser, Magdalena Crhanova, Daniela Karasova, Hana Stepanova, Vladimir Babak, Ivan Rychlik   +7 more
doaj   +1 more source

Amplification and analysis of cDNA generated from a single cell by 5′-RACE: application to isolation of antibody heavy and light chain variable gene sequences from single B cells

BioTechniques, 2006
The technique of 5′-rapid amplification of cDNA ends (5′-RACE) is widely used to amplify unknown sequences at the 5′ end of a messenger RNA (mRNA). However, conventional 5′-RACE is inappropriate for producing cDNAs from a single cell due to the small ...
Tatsuhiko Ozawa, Hiroyuki Kishi, Atsushi Muraguchi   +2 more
doaj   +1 more source

Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fish

iScience, 2021
Summary: B cells express a unique antibody protein which comprises two pairs of immunoglobulin (Ig) heavy (H) and light (L) chains. In addition to an invariable constant (C) region, IgH and IgL chains encompass a variable (V) region mediating antigen ...
Pedro Perdiguero, Esther Morel, Patricia Díaz-Rosales, Carolina Tafalla   +3 more
doaj   +1 more source

Markers of the Variable and Constant Region of Human Lambda Immunoglobulin Chains [PDF]

European Journal of Biochemistry, 1970
52 human lambda immunoglobulin chains could be divided into two antigenic subtypes, St(+) and St(–).St(+) type chains represent a homogeneous structural group differing from St(−) type proteins by characteristic subclass specific positions in the N‐terminal sequence of amino acids. St(+) proteins follow the “Ha” type sequence.
F W, Tischendorf, M M, Tischendorf
openaire   +2 more sources

Spinal Cord Infarction Versus Idiopathic Transverse Myelitis: Clinical, Radiological, and Functional Insights From a Retrospective Cohort Study

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Introduction Spinal cord infarction (SCI) is a rare but devastating myelopathy, characterized by a high disability rate and an unfavorable prognosis. It has often been underdiagnosed and misdiagnosed as idiopathic transverse myelitis (ITM). This study aimed to describe the clinical features, radiological biomarkers, treatments, and functional ...
Zeqiang Ji, Jianlong Zhang, Yiming Shi, Shiyu Shan, Yang Du, Guangshuo Li, Ying Jin, Yani Zhang, Chuanying Wang, Yijun Lin, Yuhao Guo, Decai Tian, Xingquan Zhao, Tian Song   +13 more
wiley   +1 more source