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Issues in Immunohistochemistry [PDF]
Immunohistochemistry is a powerful technique used to localize specific molecules in tissues. Literally thousands of immunoreagents specific for an incredibly diverse number of molecules are commercially available, and some of these immunoreagents will detect minute quantities of target molecule in tissues.
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Context.— Intestinal neuronal dysplasia type B (IND B) is a complex entity involving the enteric nervous system, clinically manifested with constipation in infancy. Diagnosis has been established by histopathologic analysis of rectal biopsies. However, the criteria for the diagnosis have been questioned and modified,
Simone Antunes, Terra+2 more
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Impact of decitabine on immunohistochemistry expression of the putative tumor suppressor genes FHIT, WWOX, FUS1 and PTEN in clinical tumor samples. [PDF]
BackgroundSince tumor suppressor gene function may be lost through hypermethylation, we assessed whether the demethylating agent decitabine could increase tumor suppressor gene expression clinically.
Aldaz, Marcelo+8 more
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IHC is also a common method in basic research of disease to help understanding the distribution and localization of biomarkers and differentially expression of proteins in different parts of tissue. Creative Bioarray offers a comprehensive IHC service from project design, marker selection to image completion and data analysis.
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Diagnosis of anaplastic large-cell lymphoma in a dog using CD30 immunohistochemistry [PDF]
Anaplastic large-cell lymphoma or null-cell lymphoma is a clinical entity reported in people, classified according to the unique appearance of large pleomorphic cells that express CD30.
Garden, O A+7 more
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Developing Monoclonal Antibodies for Immunohistochemistry
The experiences of a laboratory which pioneered the application of monoclonal antibodies to diagnostic histochemistry is described. This was achieved in four key steps: (1) Monoclonal antibodies were successfully produced to replace the difficult-to ...
Jacqueline Cordell
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Levels of feline infectious peritonitis virus in blood, effusions, and various tissues and the role of lymphopenia in disease outcome following experimental infection. [PDF]
Twenty specific pathogen free cats were experimentally infected with a virulent cat-passaged type I field strain of FIPV. Eighteen cats succumbed within 2-4 weeks to effusive abdominal FIP, one survived for 6 weeks, and one seroconverted without outward ...
Eckstrand, Chrissy+4 more
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CD45 Immunohistochemistry in Mouse Kidney
CD45 is a pan-leukocyte marker, and CD45 stain is widely used to determine the extent of inflammatory cell infiltration and its association with tissue injury.
Shirong Zheng, Paul Epstein
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Automated Segmentation of Cells with IHC Membrane Staining [PDF]
This study presents a fully automated membrane segmentation technique for immunohistochemical tissue images with membrane staining, which is a critical task in computerized immunohistochemistry (IHC).
Acquaviva, Andrea+3 more
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Follicular complex may predict reproductive tactics in siluriform fishes
In this study, we evaluated the morphology and morphometry of the layers that make up the follicular complex surrounding mature oocytes in the six fish species Auchenipterichthys longimanus, Ageneiosus ucayalensis, Hypophthalmus marginatus, Baryancistrus
Ivana Kerly S. Viana+5 more
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