Results 81 to 90 of about 114,137,818 (305)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

In vitrofermentation characteristics of corn and sorghum silages

Italian Journal of Animal Science, 2007
Aim of the study was to compare corn and sorghum forages, before and after ensiling, in terms of chemical-nutritional characteristics and fermentation kinetics, to substitute corn silage with sorghum silage. The trial was performed using sorghum (Sorghum vulgare) and corn (Zea mais) incubated in vitro at 39°C for 120 h with buffalo (Bubalus bubalis)
CALABRO', SERENA, TUDISCO, RAFFAELLA, GROSSI M, BOVERA, FULVIA, CUTRIGNELLI, MONICA ISABELLA, GUGLIELMELLI A, PICCOLO, VINCENZO, INFASCELLI, FEDERICO   +7 more
openaire   +3 more sources

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

In vitro screening of probiotic lactic acid bacteria and prebiotic glucooligosaccharides to select effective synbiotics [PDF]

, 2010
Probiotics and prebiotics have been demonstrated to positively modulate the intestinal microflora and could promote host health. Although some studies have been performed on combinations of probiotics and prebiotics, constituting synbiotics, results on ...
Julien Grimoud, Durand, Henri, Henri Durand, Monsan, Pierre, Courtin, Céline, Grimoud, Julien, Theodorou, Vassilia, Christine Roques, Pierre Monsan, Françoise Ouarné, Ouarné, Françoise, Céline Courtin, Roques, Christine, Rogues, Christine, Vassilia Theodorou   +14 more
core   +1 more source

Predicting methane production in dairy cows [PDF]

, 2013
Methane is a potent greenhouse gas, to which enteric fermentation from ruminants contributes significantly. Reliable and accurate predictions of methane (CH₄) production from dairy cows would be of interest to develop mitigation strategies and for ...
Ramin, Mohammad
core   +1 more source

In vitro digestion and fermentation behaviors of polysaccharides from Choerospondias axillaris fruit and its effect on human gut microbiota

Current Research in Food Science
Choerospondias axillaris fruit has attracted more and more attention due to its various pharmacological activities, which are rich in polysaccharides. This study investigated the in vitro saliva-gastrointestinal digestion and fecal fermentation behaviors
Jinjiao Dong, Wenjun Wang, Guodong Zheng, Nansheng Wu, Jingjing Xie, Shiyi Xiong, Panting Tian, Jingen Li   +7 more
doaj   +1 more source

Microbial Fermentation of Rice Straw: Nutritive Composition and In Vitro Digestibility of the Fermentation Products [PDF]

Applied Microbiology, 1975
Rice straw was fermented with Cellulomonas sp. and Alcaligenes faecalis . Microbial cells and undigested residue, as well as chemically treated (NaOH or NH 4 OH) and untreated straws, were analyzed for nutrient ...
openaire   +2 more sources

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Effects of collagen gel configuration on behaviour of vascular smooth muscle cells in vitro: association with vascular morphogenesis.

, 2000
The growth, behavior, and contractile protein expression of rabbit aortic smooth muscle cells (SMC) grown on, between layers, or within a collagen gel was investigated by confocal laser scanning fluorescence microscopy and Western analysis.
Campbell, JH, Hayward, IP, Rolfe, BE, Campbell, Gordon R., Campbell, GR, Campbell, Julie H., Hayward, Ian Philip, Rolfe, Barbara E., Song, J, Song, Jian   +9 more
core   +1 more source

Microbial community development in a dynamic gut model is reproducible, colon region specific, and selective for Bacteroidetes and Clostridium cluster IX [PDF]

, 2010
Dynamic, multicompartment in vitro gastrointestinal simulators are often used to monitor gut microbial dynamics and activity. These reactors need to harbor a microbial community that is stable upon inoculation, colon region specific, and relevant to in ...
Marzorati, M., Sam Possemiers, Sahar El Aidy, Kleerebezem, M., Verstraete, Willy, Smidt, H., Marzorati, Massimo, Philippe Gérard, Rabot, S., Charlotte Grootaert, Verstraete, W., Aidy, S.F., El, Erwin Zoetendal, Hauke Smidt, Possemiers, Sam, Zoetendal, Erwin, Kleerebezem, Michiel, van der Wiele, T., Sylvie Rabot, Grootaert, C., Michiel Kleerebezem, Possemiers, S., Massimo Marzorati, Derrien, Muriel, Pieter Van den Abbeele, Tom Van de Wiele, Derrien, M.M.N., Rabot, Sylvie, Grootaert, Charlotte, van den Abbeele, P., Bruneau, A., Wiele, T., van der, Willy Verstraete, Zoetendal, E.G., El Aidy, Sahar, Gerard, Philippe, Abbeele, P., van den, Muriel Derrien, Smidt, Hauke, Van de Wiele, Tom, Gérard, P., Van den Abbeele, Pieter, Bruneau, Aurelia, El Aidy, S.F., Aurélia Bruneau   +44 more
core   +1 more source